Volume 9, Issue 1 (5-1995)                   Med J Islam Repub Iran 1995 | Back to browse issues page

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From the' Dept. of Mycology and Parasitology, Isfahan University of Medical Sciences, Isfahan
Abstract:   (2597 Views)
In this study techniques for laboratory identification of dermatophyte fungi through protoplast hybridization were established. Firstly, auxotrophic mutants of different species of microsporum and trichophyton were induced and identified. Secondly, protoplasts from these mutants were isolated by digestion of their mycelium with Novozyme 234 using CaCI, (O.4M) as an osmotic stabilizer and glycine + HCI (pH 4.5) as the buffer system. Thirdly, isolated protoplasts from different species were fused using a solution containing 35% polyethylene glycol, I M KCI, 0.05 M CaCI, and 0.05 M glycine with pH 6.1. Afterwards, the fusion products were plated onto minimal and complete media. It was found that protoplasts from different auxotrophic mutants from the same species hybridized and complemented each other and grew on both minimal and complete media. whereas mutants from different species did not have the ability to complement each other and therefore grew only on complete media. Information obtained in this study may prove useful for definite identification of suspected species of dennatophytes other than morphological criteria in laboratories.
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Type of Study: Original Research | Subject: Parasitology and Mycology