From the Institute of Biochemistry and Biophysics, Tehran University, Tehran, Islamic Republic of Iran.
Abstract: (3412 Views)
The hemopoietic activity of the human placenta was studied in vitrc under
various conditions in order to assess the optimal conditions of protein synthesis
and colony stimulating factor (CSF) production by this tissue. The hemopoietic
activity of placental tissue was assayed by the semi -solid agar medium technique.
In order to obtain maximum hemopoietic activity, various stimulators were
examined and it was determined that lipopolysaccharide was the most potent
stimulator. Inhibitors of transcription and translation were also examined alone
and in the presence of stimulators. They potently suppressed hemopoietic activity.
Pulse-chase studies with the use of 3H -leucine were used to determine the kinetics
of protein synthesis of placental tissue ill vitro.