Volume 29, Issue 1 (1-2015)                   Med J Islam Repub Iran 2015 | Back to browse issues page

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Dowlatshahi K, Ajami M, Pazoki-Toroudi H, Hajimiresmaiel S J. ATP-dependent potassium channels are implicated in simvastatin pretreatment-induced inhibition of apoptotic cell death after renal ischemia/reperfusion injury. Med J Islam Repub Iran. 2015; 29 (1) :263-272
URL: http://mjiri.iums.ac.ir/article-1-2749-en.html
Department of Cardiology, Iran University of Medical Sciences, Tehran, Iran. , chancellor@iums.ac.ir
Abstract:   (2848 Views)

 

Background: Simvastatin is a widely used medication in cardiac care. Here we evaluate the role of ATP sensitive potassium (KATP) channels in simvastatin induced renal protection after renal ischemia/reperfusion (I/R) injury.

 

 Methods: A total of 81 male Wistar rats, were treated with simvastatin (10 and 20mg/kg/day gavage, one week). Some groups received glibenclamide (KATP channel inhibitor 5mg/kg) before ischemia (45min) and reperfusion (24h). Finally the kidneys were processed for histological analysis and measurement of biochemical parameters including tissue malondialdehyde (MDA), blood urea nitrogen (BUN), fractional excretion of sodium (FENa), creatinine clearance rate (CCr) and Bcl2-associated X protein (Bax) expression.

 

 Results: IR significantly increased serum Cr (p< 0.01) and BUN levels (p< 0.01), elevated FENa (p<0.01) and tissue MDA (p<0.01), and decreased CCr (p< 0.01) and induced histological damage. Bax pro-apoptotic protein was upregulated in renal tissue after I/R injury and downregulated in simvastatin pretreated group. Simvastatin at doses of 10 and 20mg/kg/day significantly reduced serum Cr and BUN levels (p< 0.05 vs. IR group), tissue MDA contents and FENa (p< 0.05 vs. I/R) and increased CCr (p< 0.05 vs. IR). Renal tissue injury was improved only in simvastatin 20mg/kg/day group (p< 0.05). Glibenclamide significantly abolished protective effects of simvastatin and increased serum Cr and BUN and FENa and decreased CCr (p< 0.05). It also abolished the effects of simvastatin on tissue injury and MDA contents and downregulated the Bax protein after IR injury (p< 0.05).

 

 Conclusion: Opening of KATP channels is essential for simvastatin-induced renal protection against I/R injury.

 

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Type of Study: Original Research | Subject: Physiology

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