Volume 31, Issue 1 (1-2017)                   Med J Islam Repub Iran 2017 | Back to browse issues page




DOI: 10.18869/mjiri.31.12

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Rafiei Sefiddashti R, Sharafi S M, Ebrahimi S A, Akhlaghi L, Moosavi A, Eskandarian A, et al . Antibody response to glycan antigens of hydatid cyst fluid, laminated layer and protoscolex of Echinococcus granulosus. Med J Islam Repub Iran. 2017; 31 (1) :66-70
URL: http://mjiri.iums.ac.ir/article-1-3439-en.html

Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran.
Abstract:   (895 Views)

Background: Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an
important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the
cyst, has been investigated.
Methods: The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), cyst fluid (CF) and protoscolex
(PS) antigens of E. Granulosus using ELISA and western immunoblotting tests. Thin-layer chromatography and ß-elimination
were used for glycan purification.
Results: Both hydatid cyst and normal human sera reacted with hydatid cyst fluid, protoscolices, laminated layer, glycoprotein and
glycolipid antigens. The most antigen-antibody reaction was related to CF and PS antigens, and LL antigens had the minimal reaction
with the sera. Thin layer chromatography (TLC) of the antigens showed presence of many glycan bands in the laminated layer.
Conclusion: The parasite may elaborate different glycan antigens in LL to evade host immune response.

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Type of Study: Original Research | Subject: Parasitology and Mycology