Volume 30, Issue 1 (1-2016)                   Med J Islam Repub Iran 2016 | Back to browse issues page

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Rostami A, Moosavi S A, Changizi V, Abbasian Ardakani A. Radioprotective effects of selenium and vitamin-E against 6MV X-rays in human blood lymphocytes by micronucleus assay. Med J Islam Repub Iran 2016; 30 (1) :469-475
URL: http://mjiri.iums.ac.ir/article-1-3713-en.html
Department of Technology of Radiology and Radiotherapy, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran. , changizi@sina.tums.ac.ir
Abstract:   (4183 Views)

Background: Critical macromolecules of cells such as DNA are in exposure to damage of free radicals that induced from the interaction of ionizing radiation with biological systems. Selenium and vitamin-E are natural compounds that have been shown to be a direct free radical scavenger. The aim of this study was to investigate the radioprotective effect of selenium and vitamin-E separately and synergistically against genotoxicity induced by 6MV x-rays irradiation in blood lymphocytes.

  Methods: Fifteen volunteers were divided into three groups include A, B and C. These groups were given selenium (800IU), vitamin-E (100mg) and selenium (400IU) + vitamin-E (50mg), respectively. Peripheral blood samples were collected from each group before (0hr) and 1, 2 and 3hr after selenium and vitamin-E administration (separately and synergistically). Then the blood samples were irradiated to 200cGy of 6MV x-rays. After that lymphocyte samples were cultured with mitogenic stimulation to determine the chromosomal aberrations with micronucleus assay in cytokinesis-blocked binucleated cells.

  Results: The lymphocytes in the blood samples collected at one hr after ingestion selenium and vitamin-E, exposed in vitro to x-rays exhibited a significant decrease in the incidence of micronuclei, compared with control group at 0hr. The maximum protection and decrease in frequency of micronuclei (50%) were observed at one hr after administration of selenium and vitamin-E synergistically.

  Conclusion: The data suggest that ingestion of selenium and vitamin-E as a radioprotector substance before exposures may reduce genetic damage caused by x-rays irradiation.

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Type of Study: Original Research | Subject: Radiography

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