Volume 18, Issue 2 (9-2004)                   Med J Islam Repub Iran 2004 | Back to browse issues page


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BASSERI H, RATCLIFFE N. IN VITRO INHIBI TI ON OF ATTACHMENT OF T HE AMERICAN TRYPANOSOME, TRYPANOSOMA RANGELI ON THE SALIVARY GLAND OF I TS VECTOR, RHODNIUS PROLIXUS, USING THE PARASITE EXTRACT AND PARTI AL PURIFICATION OF AN ATTACHMENT INHIBITOR PROTEIN. Med J Islam Repub Iran. 2004; 18 (2) :159-166
URL: http://mjiri.iums.ac.ir/article-1-637-en.html

the Department of Medical Entomology. School of Public Health, Tehran University of Medical Sciences, P0. Box 14155-6446. Tehran, Iran , hamid_basseri@hotmail.com" hamid_basseri@hotmail.com
Abstract:   (1860 Views)
The life cycle of the American trypanosome, Trypanosoma rangeli, in its invertebrate host, Rhodnius prolixus, is completed by invasion into the insect salivary glands. As a prerequisite, there is an initial recognition of the salivary gland receptors for the adhesion/invasion by T rangeli. Therefore, the purpose of this study was to investigate the putative receptor molecules on either the salivary gland or parasites surfaces, which mediate the adhesion/invasion. Initially, the possible agglutination activity ofT rangeli epimastigotes against blood cell was examined. Then, in order to investigate the role of any receptor ligands on the surface of T rangeli in adhesion/invasion of the parasite, GlcNAc was preferentially used to isolate a ligand protein. A 60kDa protein bound to the GlcNAc column and was isolated from T rangeli (Tr-60) and the purity of this protein was confirmed by reverse phase HPLC. Tr-60 also exhibited a potent parasite inhibitory effect indicating that this protein (most probably a glycoprotein) may act as a ligand receptor. In vivo, the development and invasion of T rangeli into the salivary gland was not interfered with by the presence of a sugar inhibitor of the R. prolixus hemolymph lectin
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