Volume 15, Issue 4 (2-2002)                   Med J Islam Repub Iran 2002 | Back to browse issues page

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ASSMAR M, SOLEIMANI M, PIYAZAK N, OREIZI F, HOSSEINI S H, ZAMANI Z. P REPARATION OF A NEW MEDIUM FOR IN VITRO CULTURE OF BORRELIA MICROTTI AND BORRELIA PERSICA. Med J Islam Repub Iran. 2002; 15 (4) :227-230
URL: http://mjiri.iums.ac.ir/article-1-787-en.html
From the Departments of Parasitology, and Biochemistry, Pasteur institute 0f Iran, Tehran, l.R. Iran. , Zamani@institute.pasteur.ac.ir
Abstract:   (2059 Views)
Borrelia microtti and Borrelia persica are two Iranian strains of spirochetes whose vectors are Ornithodorous tholozani and Ornithodorous erraticus which are responsible for relapsing fever. BSK medium has been used for in vitro culture of other strains of borrelia, although the two mentioned strains could not be successfully cultured in it. We have modified BSK medium by using guinea pig and fetal calf serum instead of rabbit serum. It was observed that for in vitro culture of Borrelia persica, guinea pig serum and for Borrelia microtti, fetal calf serum show the best growth.
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