Volume 13, Issue 1 (5-1999)                   Med J Islam Repub Iran 1999 | Back to browse issues page


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SHOKOHI T, SILVER J C. SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION. Med J Islam Repub Iran. 1999; 13 (1) :61-66
URL: http://mjiri.iums.ac.ir/article-1-974-en.html

From the Department of Parasitology, Mycology and Entomology. Mazandaran University of Medical Sciences, Sari, Islamic Republic of Iran
Abstract:   (1695 Views)
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus species isolated from bronchoalveolar lavage (BAL) fluid from patients with TA. In single PCR using the outer primers a specific 384-bp fragment was amplified. Similarly, y nested PCR with inner primers, a 357-bp fragment was amplified with DNA from Aspergillus fumigatus but not from the other microorganisms. The Southern blot hybridizations confirm the specificity of the PCR procedure for A. fumigatus using the cloned 374-bp PCR product probe. In conclusion, the nested PCR method appears to be quite rapid and specific.
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