TY - JOUR T1 - Immunophenotype of peripheral blood lymphocytes following thermal injury in patients TT - JF - MJIRI JO - MJIRI VL - 24 IS - 2 UR - http://mjiri.iums.ac.ir/article-1-163-en.html Y1 - 2010 SP - 96 EP - 102 KW - Burn KW - T lymphocytes KW - B lymphocytes KW - NK cells KW - CD4+/CD8+ ratio N2 - AbstractBackgroundcontributes substantially to patient morbidity and mortality.In this study we investigatedthe range and distribution of T-lymphocyte. Subsets CD3helper/inducer cell,.th ), CD8: Severe immunosuppression occurs after large thermal burn and probably+ (T cells) CD4+ (T+ (T suppressor /Cytotoxic cells ,TS/C), CD3+CD4thermal injury.+/CD3+CD8+ ratio, CD19+ (B cells) and CD16+ (NK cells ) in patiens followingMethodsstudied.The total body surface area of the burn injury, ranged from 30 to >70%. Wholeblood samples were collected at three and seven days postburn. Partec flowcytometrysystem and triple color flowcytometry reagents (Dako Co), were used to evaluate peripheralblood lymphocytes population of patients admitted at the Motahary Burn Centerin Tehran.: Forty male, aging 18-60 years with major thermal injury wereResultsreduction in relative number of CD3postburn.CD4range in seven days following injury. CD19burn patients at both three and seven days. The number of CD16declined in three days and moderately increased on day seven, following injury.Thus, the data showed that thermal burn injury suppressed T-lymphocyte subsets proliferationin various days .In addition, all compartments of showed phenotypic changesin the 3th and seventh days after burn, in different groups of age. Thermal burn injurysuppressed T cell subsets proliferation on day 3 and 7 postburn, when compared to normalcontrols. (P M3 ER -