RT - Journal Article T1 - A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS JF - MJIRI YR - 1996 JO - MJIRI VO - 10 IS - 3 UR - http://mjiri.iums.ac.ir/article-1-1190-en.html SP - 233 EP - 235 AB - We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymerase chain reaction (PCR) was performed with primers for a 530-base pair (bp) fragment of the gene encoding the 36 kDa antigen of M. leprae. All the specimens were found to be PCR -positive, suggesting the efficiency of the present DNA extraction method. LA eng UL http://mjiri.iums.ac.ir/article-1-1190-en.html M3 ER -