AU - MOUSAVI HOSSEINI, KAMRAN AU - POURMOKHTAR, MOJGAN AU - DINAR VAND, RASSOUL AU - REZVAN, HOURI AU - JALILI, MOHAMMAD ALI TI - PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA PT - JOURNAL ARTICLE TA - MJIRI JN - MJIRI VO - 17 VI - 4 IP - 4 4099 - http://mjiri.iums.ac.ir/article-1-668-en.html 4100 - http://mjiri.iums.ac.ir/article-1-668-en.pdf SO - MJIRI 4 ABĀ  - As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-D.35M NaCI buffer, pH 8.1, resulting in recovery of 85 % IgM, 84% IgA and 33 % IgG. The comparison of our results with immunoglobulins' percentage in plasma indicates that IgM and IgA -enrichment was obtained by three times. CP - IRAN IN - LG - eng PB - MJIRI PG - 315 PT - Original Research YR - 2004