RT - Journal Article T1 - A STUDY ON THE APPLICATION AND EFFICACY OF SOLVENT-DETERGENT (SID) TREATMENT IN THE PROCESS OF PURIFYING FACTOR VII FROM PROTHROMBIN COMPLEX JF - MJIRI YR - 2002 JO - MJIRI VO - 16 IS - 3 UR - http://mjiri.iums.ac.ir/article-1-751-en.html SP - 179 EP - 182 K1 - Human Factor VII concentrate K1 - viral inactivation K1 - solvent-detergent treatment. AB - The risks of transmitting viral infection by blood and plasma-derived products have long been known and still remain an area of concern. In this study, in the process of purifying human factor VII from prothrombin complex, SID treatment using tri-n-butyl phosphate and Tween 80 was employed and its capability and efficacy was studied. The results indicated that the process did not affect the biological function of the essential coagulation factors studied. In addition, the process was shown to be effective on enveloped viruses, where its inactivation factor for the model used, Herpes Simplex type I, was 5.5 logs. However, the procedure was not effective on poliovirus which was used as a model for non-enveloped viruses. It is therefore concluded that for increasing viral safety it is best to apply at least one more inactivating procedure which will be effective on non-enveloped viruses as well. LA eng UL http://mjiri.iums.ac.ir/article-1-751-en.html M3 ER -