RT - Journal Article T1 - HEPATOMA McARDLE-RH7777 CELLS HAVE THE SAME RESPONSE AS NORMAL RAT HEPATOCYTES TO BOTH DIBUTYRYL-cAMP AND ANTICALMODULINW-7 JF - MJIRI YR - 2002 JO - MJIRI VO - 15 IS - 4 UR - http://mjiri.iums.ac.ir/article-1-785-en.html SP - 209 EP - 217 K1 - Calmodulin K1 - Cyclic AMP K1 - Hepatocyte K1 - McArdle cells and VLDL AB - The effects of cAMP-analogue dibutyryl-cAMP and anticalmodulin W-7 were studied on de novo synthesis and secretion of lipids in cultures of hepatoma McArdle RH7777 cells and normal rat hepatocytes. Dibutyryl-cAMP and W -7 separately caused a significant decrease in the secretion of de novo synthesized triacyl [3H]glycerol in both cultures of McArdle cells and rat hepatocytes. The inhibitory effects of dibutyryl-cAMP and W-7 were concentration-dependent and appeared at the lowest concentration examined, 5JlM and 20JlM respectively. DibutyrylcAMP at a concentration of 50 JlM and W -7 (20 µM) suppressed the secretion of triacylglycerol by approx. 38% (p LA eng UL http://mjiri.iums.ac.ir/article-1-785-en.html M3 ER -