AU - BAMDAD, T AU - RODSTAI, MH AU - SOLIMANDJAHI, H AU - MALEKANEH, M TI - APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY PT - JOURNAL ARTICLE TA - MJIRI JN - MJIRI VO - 15 VI - 3 IP - 3 4099 - http://mjiri.iums.ac.ir/article-1-801-en.html 4100 - http://mjiri.iums.ac.ir/article-1-801-en.pdf SO - MJIRI 3 ABĀ  - A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate was taken as a positive result. The adapted DIA was applied to test 105 serum samples. The sensitivity and specificity and immune titer of DIA were compared with hemagglutination inhibition (HI) test. CP - IRAN IN - LG - eng PB - MJIRI PG - 171 PT - Original Research: Basic Science in Medicine YR - 2001