TY - JOUR T1 - APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY TT - JF - MJIRI JO - MJIRI VL - 15 IS - 3 UR - http://mjiri.iums.ac.ir/article-1-801-en.html Y1 - 2001 SP - 171 EP - 174 KW - Rubella virus. dot immunoassay ( DIA) KW - nitrocellulose paper. hemagglutination inhibition (HI) N2 - A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate was taken as a positive result. The adapted DIA was applied to test 105 serum samples. The sensitivity and specificity and immune titer of DIA were compared with hemagglutination inhibition (HI) test. M3 ER -