%0 Journal Article %A MALAKANEH, M %A RASAEE, MJ %A MADANI, K %A POURFATHOLLAH, AA %T ENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL %J Medical Journal of the Islamic Republic Of Iran %V 12 %N 3 %U http://mjiri.iums.ac.ir/article-1-1004-en.html %R %D 1998 %K %X An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assay is completed within 4 hr. The limit of detection was 10 pg of neopterin with a sensitivity range between 15-10000 pg. A low level of cross-reactivity with other pteridines was noted (biopterin %> http://mjiri.iums.ac.ir/article-1-1004-en.pdf %P 259-264 %& 259 %! ELISA for Neopterin Measurement %9 Original Research: Basic Science in Medicine %L A-10-298-455 %+ From the Department of Biochemistry, Birjand University of Medical Sciences, Birjand %G eng %@ 1016-1430 %[ 1998