TY - JOUR T1 - THE INTERACTION OF RADIATION AND INTERCALATING AGENTS IN NORMAL BONE MARROW CELLS AS EVALUATED BY SPLEEN COLONY ASSAY TECHNIQUE: THE EFFECTS OF BLEOMYCIN SULFATE AND ACT INOMYCIND. TT - JF - MJIRI JO - MJIRI VL - 9 IS - 2 UR - http://mjiri.iums.ac.ir/article-1-1340-en.html Y1 - 1995 SP - 137 EP - 145 KW - Biological effects. Radiation KW - Bleomycin sulfate KW - Actinomycin D KW - Bone marrow cells N2 - The relationship between the way in which normal hemopoietic stem cells respond to irradiation alone or in the presence of bleomycin sulfate (BLM-S) and actinomycin 0 (ACT-D) was investigated. Single doses of BLM-S at 0.3 mg/kg and ACT-O at 0.10 mg/kg body weight were injected intravenously 1-6 hours prior to whole body irradiation and treatment was repeated twice more with time intervals. When assessed by survival of spleen colony forming units (CFU-S) of bone marrow cells (BMC), BLM-S alone caused only 10% reduction in survival compared to controls. There was not a significant difference in survival fraction (SF) when treatment with BLM-S was repeated twice more. On the other hand, ACT-O alone caused a 45% reduction in SF after the first injection and only a 10% reduction after the third injection. Increase in survival might be due to resistance induced in BMC after treatments with the drugs. The difference between the 'SF of BMC of mice exposed to doses of 1-3 Gy whole body irradiation was statistically significant with a p-value M3 ER -