%0 Journal Article %A BASSERI, HR %A RATCLIFFE, NA %T IN VITRO INHIBI TI ON OF ATTACHMENT OF T HE AMERICAN TRYPANOSOME, TRYPANOSOMA RANGELI ON THE SALIVARY GLAND OF I TS VECTOR, RHODNIUS PROLIXUS, USING THE PARASITE EXTRACT AND PARTI AL PURIFICATION OF AN ATTACHMENT INHIBITOR PROTEIN %J Medical Journal of the Islamic Republic Of Iran %V 18 %N 2 %U http://mjiri.iums.ac.ir/article-1-637-en.html %R %D 2004 %K Tlypanosoma rangeli, Rhodnius prolixus, Salivary glands, P roteins, attachment., %X The life cycle of the American trypanosome, Trypanosoma rangeli, in its invertebrate host, Rhodnius prolixus, is completed by invasion into the insect salivary glands. As a prerequisite, there is an initial recognition of the salivary gland receptors for the adhesion/invasion by T rangeli. Therefore, the purpose of this study was to investigate the putative receptor molecules on either the salivary gland or parasites surfaces, which mediate the adhesion/invasion. Initially, the possible agglutination activity ofT rangeli epimastigotes against blood cell was examined. Then, in order to investigate the role of any receptor ligands on the surface of T rangeli in adhesion/invasion of the parasite, GlcNAc was preferentially used to isolate a ligand protein. A 60kDa protein bound to the GlcNAc column and was isolated from T rangeli (Tr-60) and the purity of this protein was confirmed by reverse phase HPLC. Tr-60 also exhibited a potent parasite inhibitory effect indicating that this protein (most probably a glycoprotein) may act as a ligand receptor. In vivo, the development and invasion of T rangeli into the salivary gland was not interfered with by the presence of a sugar inhibitor of the R. prolixus hemolymph lectin %> http://mjiri.iums.ac.ir/article-1-637-en.pdf %P 159-166 %& 159 %! Inhibition of T rangeli Attachment on R. prolixus %9 Original Research: Basic Science in Medicine %L A-10-298-96 %+ The Department of Medical Entomology. School of Public Health, Tehran University of Medical Sciences, P0. Box 14155-6446. Tehran, Iran %G eng %@ 1016-1430 %[ 2004