RT - Journal Article T1 - Determination of Helicobacter pylori virulence-associated genes in duodenal ulcer and gastric biopsies JF - MJIRI YR - 2017 JO - MJIRI VO - 31 IS - 1 UR - http://mjiri.iums.ac.ir/article-1-4274-en.html SP - 555 EP - 559 K1 - Helicobacter pylori K1 - Iran K1 - Virulence factors K1 - Multiplex PCR K1 - Duodenal ulcers AB - Background: Helicobacter pylori (H. pylori or Hp) has been strongly associated with the peptic ulcer diseases, chronic gastritis, ulcers, and gastric cancer. Genes associated with pathogenicity have been designated for H. pylori, and some of them appear to be related to more severe clinical consequences of the infection. The present study was conducted to determine cagA, vacA, cagE, iceA1, oipA, and iceA2 genes in H. pylori strains isolated from gastroduodenal patients, who referred to Shariati hospital in Tehran, Iran. Methods: Gastric biopsy specimens were collected during endoscopy from patients, who referred to the Shariati hospital in Tehran, Iran during January and November 2015. After isolation of H. pylori from the biopsy culture, genomic DNA was extracted and subsequently used to identify H. pylori and virulence genes using specific primers. Results: The isolation rate of H. pylori strains was 65.7% (169/257). The frequency of cagA, vacA, cagE, iceA1, oipA, and iceA2 was 143 (% 84.6), 169 (100%), 131 (77.5%), 97 (57.3%), 89 (52.6%), and 72 (42.6%), respectively. Conclusion: In this study, a significant difference was observed between investigated genes and strains isolated from PUD and GC patients (p<0.05). LA eng UL http://mjiri.iums.ac.ir/article-1-4274-en.html M3 10.14196/mjiri.31.95 ER -