TY - JOUR JF - MJIRI JO - Med J Islam Repub Iran VL - 33 IS - 1 PY - 2019 Y1 - 2019/2/01 TI - Association of HCV genotype with viral load among Iranian blood donors: a penalized logistic regression TT - N2 - Background: Hepatitis C virus (HCV) is a blood born virus and the leading cause of advanced hepatitis disease. HCV genotype 3a is predominant among Iranian blood donors. The aim of this study was to evaluate the relationship between HCV genotype and HCV viral load. Methods: In this analytical cross-sectional study 106 anti-HCV positive and HCV RNA positive blood donors referred to Iranian blood centers across the county were entered. HCV viral loads were determined by an in-house one step Taq Man Real-Time RT-PCR assay. Penalized logistic regression was performed for data analysis. STATA software version 13 was used for statistical analysis. Results: The mean age was 37.94 ± 9.04 years ranged from 19 to 58 years. Male gender included 104 (98.1%) of subjects. 31, 10 and 65 subjects were infected with genotypes1a, 1b, and 3a, respectively. The mean viral load was 1.44 × 106 ± 4.5× 105 IU/ml. HCV viral load was not significantly different among subjects infected with HCV genotypes 1, 1.49 × 10 6 ± 4.57 × 10 6 IU/ml compare to genotype 3, 1.40 × 10 6 ± 5.58 4.58 × 106 IU/ml (p=0.93). Conclusion: Although not significant, the frequency of subjects with high viral load (> 800,000 IU/ml) was higher in subjects infected with genotype 3 than those of genotype 1. No associations were found between demographic characteristics and HCV genotype. Although the study was unable to find any association between HCV genotype and HCV viral load/ HCV viral load group, it highlighted the role of high viral load in the high circulation of HCV genotype 3a among Iranian blood donors. SP - 919 EP - 923 AU - Ranjbar Kermani, Fahimeh AU - Amini Kafi-Abad, Sedigheh AU - Mousavi Hosseini, Kamran AU - Maghsudlu, Mahtab AU - Sharifi, Zohreh AU - Mansournia, Mohammad Ali AD - Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran KW - Blood donors KW - Hepatitis C KW - Viral load KW - Genotype KW - sequencing KW - Iran UR - http://mjiri.iums.ac.ir/article-1-5718-en.html DO - 10.47176/mjiri.33.149 ER -