Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1377
8
1
gregorian
1998
11
1
12
3
online
1
fulltext
en
ENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
An enzyme-linked immunosorbent assay for neopterin using penicillinase as
marker enzyme is reported here by polyclonal antibodies against neopterin conjugated
to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions
were purified and coated on wells of microtiter plates. A chain heterology was
introduced in neopterin derivative and conjugated to penicillinase. The assay is
completed within 4 hr. The limit of detection was 10 pg of neopterin with a sensitivity
range between 15-10000 pg. A low level of cross-reactivity with other pteridines was
noted (biopterin <0.05%, pteroic acid <0.05%, and pterin <5%). The sensitivity and
selectivity observed in the assay may be attributable to the selection of penicillinase
as the enzyme marker and the element of conformation (heterology between the
antigen-linked and enzyme-conjugated hapten).
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-455&slc_lang=en&sid=1
M
MALAKANEH
20031947532846004784
20031947532846004784
Yes
From the Department of Biochemistry, Birjand University of Medical Sciences, Birjand
MJ
RASAEE
20031947532846004785
20031947532846004785
No
the Departments of Biochemistry , Tarbiat Modarres University of Medical Sciences, Tehran
K
MADANI
20031947532846004786
20031947532846004786
No
the Razi Research Institute, Hesarak, Islamic Republic of Iran.
AA
POURFATHOLLAH
20031947532846004787
20031947532846004787
No
the Departments of Immunology, Tarbiat Modarres University of Medical Sciences