Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1377
8
1
gregorian
1998
11
1
12
3
online
1
fulltext
en
PURIFICATION AND PARTIAL CHARACTERIZATION O F PEROXIDASES FROM CULTIVATED RAPHANUS SATIVUS L. VAR. CICIL
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Two peroxidases (EC. 1.11.1.7), POD I and POD II were purified from the roots
of cultivatedRaphanus sativus L. Var. Cicil by one step ion-exchange chromatography
after fractionation by acetone. The molecular weight of these enzymes were 43000
and 41000 Daltons and RZ 1.2 and 2.0 for POD I and POD II, respectively. Both
enzymes consisted of a single polypeptide chain on SDS-PAGE. The maximum
activity of POD I was observed at pH 4.6 and 30°C and for POD II, at pH 6.5 and
60°C. The Km value of POD I for H202 was 7.26 mM and for POD II, 2 mM toward
o-dianisidin. Both isoenzymes were stable for 48 hours in temperatures up to 40°C
and stable in pH 4-8 for 3 hours.
Peroxidase, Horseradish, Purification, Characterization.
273
277
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-457&slc_lang=en&sid=1
M
TABATABAIE YAZDI
20031947532846004790
20031947532846004790
Yes
From the Department of Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences,
S
KHALEGHPARAST
20031947532846004791
20031947532846004791
No
SM
NAYEBPOUR
20031947532846004792
20031947532846004792
No