Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1377
8
1
gregorian
1998
11
1
12
3
online
1
fulltext
en
RT-PCR MEDIATED CLONING OF HUMAN GROWTH HORMONE GENE AND I TS EXPRESSION IN E. coli
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Human growth hormone (hGH) genomic sequence containing 5 exons and 4
introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used
for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH
in stably transfected cells was assayed using ELISA. Total RNA was extracted from
transfected cells and hGH cDNA was amplified by RT-PCR using specific primers.
The product thus obtained was cloned in pGEM-T vector and the presence of the
growth hormone coding region was verified by restriction enzyme analysis and
Southern blotting. The hGH cDNA fragment was cloned in pQE-30 and the
expression of hGH gene in E. coli was assayed using ELISA and immunoblotting.
In this experiment 20.9 µg/mL purified rhGH was obtained.
Human growth hormone, RT-PCR.
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290
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-459&slc_lang=en&sid=1
M
AZARNOOSH
20031947532846004795
20031947532846004795
Yes
From the Department of Biotechnology, Pasteur Institute of Iran, Tehran, Islamic Republic of Iran.
S
ZEINALI
20031947532846004796
20031947532846004796
No
SM
TABATABAEI
20031947532846004797
20031947532846004797
No
A
ZIAEE
20031947532846004798
20031947532846004798
No