Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1367
5
1
gregorian
1988
8
1
2
2
online
1
fulltext
en
DIFFERENTIATION OF MONOCYTE DERIVED DENDRITIC CELLS IN SERUM FREE CONDITIONS
Immunology
Immunology
Original Research
Original Research
Human peripheral blood monocytes (HPBM) were cultured in the
absence of human serum and were converted into a state exhibiting a high
accessory function expressed by their ability of supporting lymphocyte
proliferation. After a prolonged culture in serum free media the monocyte
derived cells were highly viable, increased in size and developed veils and
dendritiform elongatio'l1s. Paralleling the increase in accessory function, the
cells decreased in the expression of markers typical of monocytes and
macrophages (M 0), approaching the phenotype of lymphoid dendritic cells.
We here define conditions for reproducibly generating these monocyte
derived dendritic cells (m-DC) in various serum free media. This study also
shows that acidic conditions prevent M0 development and facilitate m-DC
differentiation even under serum conditions.
91
98
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-503&slc_lang=en&sid=1
ANNE CHRISTINE
BRU-CAPDEVILLE
20031947532846005613
20031947532846005613
Yes
From the Department of Immunology, University of Gouingen, West Germany
HOSSAIN
MOTTEIAN NAJAR
20031947532846005614
20031947532846005614
No
JOHANN
HINRICH PETERS
20031947532846005615
20031947532846005615
No