Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1374
7
1
gregorian
1995
10
1
9
3
online
1
fulltext
en
ANTI-BACTERIAL EFFECTS OF BISMUTH COMPOUNDS AND ITS SYNERGY WITH TETRACYCLINE AND METRONIDAZOLE ON HELICOBACTER PYLORl
Microbiology and Anatomy
Microbiology and Anatomy
Original Research
Original Research
A bacteriological investigation performed on the biopsies of 1 30 patients with
gastrointestinal disorders at the GI section of Imam Khomeini hospital concluded in
the isolation of 100 cases of Helicobacler pylori. The antibacterial effects of bismuth
salts alone and in synergy with tetracycline and metronidazole were evaluated on the
basis of disc diffusion method on 5 randomly selected pure cultures of H. pylori - the
main target - and 4 standard cultures of other bacteria including Staphylococcus
aureus (ATCC 25923), Brucella abortus (CS BPI- A 10 3) S99, Salmonella typhi (CS
BPI - 196) 0-90 I and Escherichia coli (A TCC 25922) in order to control the accuracy
of procedures.
In this research, growth inhibition zones by bismuth subcitrate (128 µg/disc
potency) were determined as 15 ± 2.4 mm diameter against H. pylori (mean ± SO),
and 15 ± 2.6, 20±3.1 and 14 ± 2.5 mm against the given standard cultures,
respectively, except for E. coli. Tetracycline (30 µg potency) produced inhibition
zones of 30 ± 2.6 mm against H. pylori and 30 ± 2.4,40 ± 2.9,25 ± 2. 3 and 21 ± 2.8
mm against the standard strains, respectively. Diameters of inhibition zones by
metronidazole were 4± I against H. pylori, and 3± 1.2, 8±2.4 and 3 ± 1. 3 mm against
the standard strains, respectively, except for the resistant E. coli.
[n another test in order to demonstrate the synergistic effects of bismuth
subcitrate together with the two mentioned antibacterial agents, a composition of 4
parts of bismuth subcitrate, one part tetracycline and 6 parts metronidazole was used
(359 µg potency). The obtained data were as follows: the growth inhibition zone
againstH. pylori was 40±3 mm and 42±2.2, 45±2.1, 35±2.6 and 27 ±2. 3 mm against
the control strains. Therefore, these results indicate that bismuth cubcitrate has a
remarkable antibacterial effect against H. pylori among the bismuth salts which
increases when used together with tetracycline and metronidazole in peptic ulcers.
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-521&slc_lang=en&sid=1
ADEL
RADJABI
20031947532846005859
20031947532846005859
Yes
From the Department of Microbiology, Medical School, Tarbiat Modarres University,Tehran, Islamic Republic 0f Iran.
BAHRAM
FATOLLAH ZADEH
20031947532846005860
20031947532846005860
No
NASRIN
MOAZAMl
20031947532846005861
20031947532846005861
No
AKBAR
MIRSALEHIAN
20031947532846005862
20031947532846005862
No
HADI
GHOFRANI
20031947532846005863
20031947532846005863
No