Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1369
8
1
gregorian
1990
11
1
4
4
online
1
fulltext
en
THE ISOLATION OF ENZYME TRANSKETOLASE FROM HUMAN ERYTHROCYTES: THE CHARACTERIZATION OF ITS QUARTERNARY STRUCTURE
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Human erythrocyte transketolase (sedoheptulose-7-phosphate:
D-glyceraldehyde-3-phosphate, glycolaldehyde transferase, E.C. 2.2.1.1.)
has been isolated from erythrocytes with a specific activity of 59.84 U/mg.
SDS-PAGE and SE-HPLC were used both as a measure of purity and as a
preparative mean to obtain a higher degree of purity. Four protein bands
corresponding to molecular weights of 32,000, 39,000, 43,000 and 60,000
were obtained in electrophoresis and SE-HPLC preparations. Activity
measurements on the two fractions obtained from SE-HPLC that contained
a monomer with the molecular weight of 32,000 and a dimeric fraction with
the molecular weight of 60,000 showed that the monomeric form of the
enzyme displays activity in the presence and absence of the TPP and Mg(II).
This activity was measured to be 14.76 U/mg in the absence of TPP and
Mg(II), and 40.24 U/mg in the presence of the cofactors. The dimeric form
showed an activity of 58.84 U/mg in the presence of the cofactors.
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-738&slc_lang=en&sid=1
ROSHANAK
RAHIMIAN
20031947532846006432
20031947532846006432
Yes
From the Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran
BIJAN
FARZAMI
20031947532846006433
20031947532846006433
No
the Department of Pharmacology; School of Medicine, Gilan University of Medical Sciences, Gilan, Islamic Republic of lran.
ABBAS
SAMADI
20031947532846006434
20031947532846006434
No
From the Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran