Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1389
5
1
gregorian
2010
8
1
24
2
online
1
fulltext
en
Polymorphism analysis of malaria susceptibility biomarkers in G6PD deficiency patients
Human Genetics
Human Genetics
Applicable
Applicable
<p> <strong> Abstract </strong></p><p> <strong> Background: </strong>Several studies suggested that some traits and polymorphisms in human genome such as G6PD deficiency and other genes have protective effects on susceptibility to malaria infection . </p><p> <strong> Methods: </strong>In present study we investigated the prevalence of TNF [1] -244GgA, TNF [1] - </p><p> 308 GgA,TNF [1] -238GgA, NOS2-954GgC, MBL54GgA, MBL 57GgA, MBL IVSI - </p><p> 5 GgA polymorphisms and G6PD variants (Mediterranean, Chatham, Cosenza, A - </p><p> (202,376) in 315 subjects with G6PD deficiency and 10 malaria patient. All the 315 subjects were selected from five provinces (Fars, Khuzestan, Esfahan, Yazd and Kerman and screened by PCR-RFLP method . </p><p> <strong> Results: </strong>The NOS2-954GgA consisted GG(40.31%), GC(53.01%), and CC(6.66% ) </p><p> where as TNF [1] -308 consisted GG(68.8%), AG(31.11% ) contents. The TNF [1] -244 </p><p> showed GG(94.60%), AG(5.39%) genotypes and the TNF [1] -238 had GG(92.69% ), </p><p> AG(6.66%), AA(0.63%) genotypes. The MBL54 polymorphism had GG(75.55%), AG (24.44%), AA(0.63%) genotypes. In MBL 57, had GG(95.23%), AG(4.76%), AA (0.63%) genotypes. The G6PD variants was indicated that Mediterranean mutation in Fars, Khuzestan, Esfahan, Yazd and Kerman provinces was 79.4%, 58%, 83/8%, 64% and 63% respectively and also, the Chatham mutation was 8.8%, 8% 4.5%,3.6% and 0% respectively. Analysis of other four mutations (Cosenza, Arures and A-202 and A-367 ) showed that none of them had those mutations . </p><p> <strong> Conclusion: </strong>Our results suggested that genotypes which causes protection against </p><p> malaria or reduction of risk for celebral malaria and death has the maximum prevalence in samples taken from the five provinces, but in the kolmogorov-smiranov test results, only NOS2-954GgC supported the theory of relation between these polymorphisms and protection against malaria . </p><p> </p>
G6PD, Polymorphism, PCR-RFLP, TNF, MBL2, NOS2, Mediterranean,
57
66
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-102&slc_lang=en&sid=1
Mohammad Reza
Noori-daloii
nooridaloii@sina.tums.ac.ir
20031947532846001947
20031947532846001947
Yes
Department of Medical Genetic, Faculty of Medicine, Tehran University of Medical Sciences, Pour Sina
Mohammad Reza
Alivand
20031947532846001948
20031947532846001948
No
Departments of Medical Genetic, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Parisa
Atef-Vahid
20031947532846001949
20031947532846001949
No
Departments of Medical Genetic, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Feyzollah
Hashemi-Gorji
20031947532846001950
20031947532846001950
No
Department of Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran
Mehrdad
Hashemi
20031947532846001951
20031947532846001951
No
Departments of Molecular Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran.