Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1396
11
1
gregorian
2018
2
1
32
1
online
1
fulltext
en
In vitro evaluation of decontamination effects on mechanical properties of fibrin membrane
Hematology
Hematology
Original Research
Original Research
<strong>Background: </strong>Tissue engineering has been investigated as a potential method for healing traumatized tissues. Biomaterials are material devices or implants used to repair or replace native body tissues and organs. The present study was conducted to evaluate the effects of decontamination methods on biological/mechanical properties and degradation/adhesion test of the platelet‑-rich fibrin (PRF) membranes to compare these properties with intact membranes as a biological biomaterial.<br>
<strong> Methods:</strong> The in vitro degradation tests were conducted by placing the equal sizes of (i) intact PRF membrane, (ii) PRF membrane sterilized by autoclave (iii), ultraviolet (UV), and (iiii) gamma irradiation in phosphate buffer solution on a shaker. The degradation profiles were expressed. Adhesion test was performed by counting adhered mouse fibroblast and sterilized fibrin membrane was compared to normal fibrin membrane by different sterilization methods.<br>
<strong> Results:</strong> The preliminary findings of sterilized PRF membranes showed that UV exposure (p<0.05) and autoclaved fibrin membranes (p<0.01) have significantly lower degradability compared to normal fibrin membranes. Gamma irradiation is similar to normal membrane in degradability. Cell adherence in all groups of fibrin membrane was significantly lower than the group without membrane, but there was no significant difference between intact and sterilized groups of fibrin membranes.<br>
<strong> Conclusion:</strong> Sterilization of fibrin membrane with different protocols does not have any adverse effects on cell adhesion; however, cell adherence is naturally very weak even in normal membranes. Also, it seems that ultraviolet ray polymerizes fibrin filaments and merges them to each other and increases the ability of fibrin membrane against degradation. Autoclaved fibrin membrane content proteins are denatured because of pressure and heat and show an increase in hardness and stability against degradation.
Fibrin, Tissue, Membrane, Mechanical phenomena, Biological phenomena, Decontamination
6
12
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-3097-1&slc_lang=en&sid=1
Taher
Akbari Saeed
t.ak.sa@hotmail.com
200319475328460036510
200319475328460036510
No
Student Research Committee, Department of Medical Laboratory Sciences, Faculty of Sirjan Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran.
Meysam
Ahmadi ZeydAbadi
meysamcell@yahoo.com
200319475328460036511
200319475328460036511
No
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Ahmad
Fatemi
ahmad.fatemi2@gmail.com
200319475328460036512
200319475328460036512
No
Department of Hematology and Blood Bank, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Alireza
Farsinezhad
farsinezhad239@yahoo.com
200319475328460036513
200319475328460036513
Yes
Department of Hematology and Laboratory Sciences, Faculty of Allied medicine, Kerman University of Medical Sciences, Kerman, Iran.