Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1396
11
1
gregorian
2018
2
1
32
1
online
1
fulltext
en
Investigation of adherent-invasive E. coli in patients with Crohn's disease
Gastroenterology
Gastroenterology
Original Research: Clinical Science
Original Research: Clinical Science
<strong>Background: </strong>Crohn's disease and Ulcerative colitis are known as inflammatory bowel disease with high morbidity which are as a result of increasing immune responses to intestinal microbiota in genetically susceptible individuals. The association of adherent invasive Escherichia coli with Crohn's disease in human has been discussed for decades. The principal aim of this study was to assess the relationship between adherent invasive Escherichia coli in Iranian patients with Crohn's disease.<br>
<strong> Methods: </strong>The presence of adherent invasive Escherichia coli DNA and viable adherent invasive Escherichia coli cells were identified through PCR and conventional culture methods, respectively. All the specimens were subsequently cultured in Hi Chrome Agar medium.<br>
<strong> Results:</strong> Using molecular assay, the invasive plasmid antigen H and invasion-association locus genes were detected from tissue samples confirming the presence of adherent-invasive Escherichia coli. The invasive plasmid antigen H was detected in 46.7% of CD and 13.3% of healthy peoples. The invasion-association locus gene was found in 36.7% of patients with Crohn's disease and 10% in individuals without IBD.<br>
<strong> Conclusion:</strong> This study demonstrated an increased frequency of adherent invasive E. coli with invasive plasmid antigen H and invasion-association locus genes from patients with CD in comparison to control individuals. Moreover, it was shown that adherent invasive E. coli with the invasive plasmid antigen H and invasion-association locus genes can act as a predisposing factor in the development of IBD.<br>
Crohn Disease, Inflammatory bowel disease, Escherichia coli, PCR
57
61
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-3650-1&slc_lang=en&sid=1
Akram
Sarabi Asiabar
Sarabi_e25@yahoo.com
200319475328460036659
200319475328460036659
No
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Hamid
Asadzade Aghdaei
Hamid.asadzadeh@gmail.com
200319475328460036660
200319475328460036660
No
Basic and Molecular Epidemiology of Gastroenterology Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Azar
Sabokbar
sabokbarr@kiau.ac.ir
200319475328460036661
200319475328460036661
No
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Mohammd Reza
Zali
nnzali@hotmail.com
200319475328460036662
200319475328460036662
No
Department of Inflammatory Bowel Disease, Research Center for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammad Mehdi
Feizabadi
mfeizabadi@tums.ac.ir
200319475328460036663
200319475328460036663
Yes
Department of Microbiology, School of Medicine, Tehran University of Medical Science, Tehran, Iran, & Thoracic Research Center, Imam Khomeini Hospital, Tehran, Iran.