Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1397
11
1
gregorian
2019
2
1
33
1
online
1
fulltext
en
In-vitro prostate cancer biomarker detection by directed conjugation of anti-PSCA antibody to super paramagnetic iron oxide nanoparticless
Biotechnology
Biotechnology
Original Research
Original Research
<strong> </strong><strong> Background: </strong>The main property of a successful conjugation of antibodies to nanoparticles is keeping the potency of antibody for binding the antigen, and an oriented conjugation can do that. Under such ground, this study was carried out to explore the efficiency of two conjugation methods in binding iron nanoparticles to an antibody produced against PSCA (prostate stem cell antigen) using in vitro labeling of PC3 cells.<br>
<strong> Methods:</strong> In this experimental study, we conjugated dextran-superparamagnetic iron oxide nanoparticles (dexSPIONs) to anti-PSCA antibody by two different methods, including targeting carbohydrate moieties in FC domain and the free amine group of amino acid side chains. Ultimately, Iron staining was done by anti-PSCA antibody-dexSPIONs in PC3 cells to detect antibody binding to the cells.<br>
<strong>Results: </strong>A strong blue dye was induced by iron staining in conjugated dexSPIONs on the membrane of PC3 cells by the former method than the second one. Moreover, cells treated with 20 nm diameters of dexSPIONs showed higher resolution of blue color than those treated with 100 nm nanoparticles.<br>
<strong> Conclusion:</strong> This oriented conjugation method promoted the efficiency of targeting tumor antigens, and the presence of iron particles might enhance MRI image intensity in vivo by targeting PSCA-overexpressing cells in future studies.<br>
Anti-PSCA antibody, Conjugation, DexSPIONs, PC3 cells
87
94
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1163-3&slc_lang=en&sid=1
Mahboube
Shahrabi Farahani
m.shahrabi2000@yahoo.com
200319475328460062852
200319475328460062852
No
Department of Medical Biotechnology, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
Monireh
Mohsenzadegan
mohsenzadegan.m@iums.ac.ir
200319475328460062853
200319475328460062853
No
Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
Jaleh
Taeb
taeb@razi.tums.ac.ir
200319475328460062854
200319475328460062854
No
Cellular & Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
Mohammad M
Farajollahi
fam-biotec@iums.ac.ir
200319475328460062855
200319475328460062855
Yes
Department of Medical Biotechnology, Faculty of Allied Medical Sciences & Cellular & Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran