Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1397
11
1
gregorian
2019
2
1
33
1
online
1
fulltext
en
Isolation of intimal endothelial cells from the human thoracic aorta: Study protocol
Clinical Biochemistry
Clinical Biochemistry
Study Protocol
Study Protocol
<strong>Background: </strong>Vessel endothelial cells are extensively applied to study the mechanism of atherosclerosis. Some cellular sources including human umbilical artery endothelial cells (HUAECs) and human umbilical vein endothelial cells (HUVECs) are mostly applied in the experimental studies. We described a method for isolating the human endothelial cells from the human thoracic aorta.<br>
<strong>Methods:</strong> Normal aortic samples were prepared from subjects with brain death in Masih Daneshvari Hospital. The endothelial cells were isolated using collagenase and were evaluated by the measurement of CD31 marker. Furthermore, the digestion efficacy was studied by vessel histological analysis, and the adhesion mechanism was investigated by leukocyte endothelial adhesion assay kit.<br>
<strong>Results and Conclusion:</strong> The isolation protocol is found as a fast and simple technique with a proper cellular load to separate the endothelial cells from the human aorta.<br>
Human aortic endothelial cell, Cell isolation, Protocol
312
316
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-4480-1&slc_lang=en&sid=1
Adeleh
Poursaleh
adelepoursaleh@yahoo.com
200319475328460062654
200319475328460062654
No
Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran
Golnaz
Esfandiari
golnaz65@yahoo.com
200319475328460062655
200319475328460062655
No
Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran
Farahnaz
Sadegh Beigee
fbeigi@gmail.com
200319475328460062656
200319475328460062656
No
Shahid Beheshti University of Medical Sciences, Masih Daneshvari Hospital, Tehran, Iran
Nahal
Eshghifar
N.eshghifar@gmail.com
200319475328460062657
200319475328460062657
No
Department of Cellular and Molecular biology, Faculty of Advanced Science and Tech-nology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Mohammad
Najafi
nbsmmsbn@iums.ac.ir
200319475328460062658
200319475328460062658
Yes
Cellular and Molecular Research Center; Biochemistry Department, Firoozabadi Hospital, & Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran