Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1399
10
1
gregorian
2021
1
1
35
1
online
1
fulltext
en
Upregulation of Ganglioside GD2 Synthase (GD2S), as a New Putative Cancer Stem Cell Marker in Breast Carcinomas
Cellular & Molecular Research
Cellular & Molecular Research
Original Research
Original Research
<strong>Background: </strong>GD2 synthase (GD2S) is the key enzyme required for ganglioside GD2 synthesis. It is commonly expressed in normal tissues and various cancers. Ganglioside GD2 is identified as a breast cancer stem cells (BCSCs) marker that promotes tumorigenesis. As GD2S has been found to be a useful molecular marker in neuroblastoma and retinoblastoma tumors, we suggest that it can be considered as a suitable candidate for the detection of CSCs in breast cancer tissues.<br>
<strong>Methods:</strong> Expression of GD2S was examined in 65 breast tumors compared to adjacent normal tissues, applying quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). The association between GD2S expression level and patients’ clinical characteristics was also assessed.<br>
<strong>Results:</strong> Our findings showed that GD2S mRNA expression was significantly higher in breast cancer tissues in comparison to normal adjacent tissue samples (4.92-fold change, p<0.001) in advanced grades (p<0.001) and stages (p<0.001). It was also shown that GD2S protein expression was significantly higher in breast cancer tissues in comparison to normal adjacent tissues (4.86-fold change, p=0.010) in advanced grades (p=0.010), stages (p=0.005) and larger tumor size (p=0.002).<br>
<strong>Conclusion:</strong> The current study showed that increased expression of GD2S in advanced breast cancer potentiates it as a promising tumor marker in these patients.
Breast cancer, Cancer Stem Cells, ELISA, GD2 Synthase, qRT-PCR
1104
1112
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-648-3&slc_lang=en&sid=1
Maryam
Mansoori
maryammansoori1495@yahoo.com
200319475328460066108
200319475328460066108
No
Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, & Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran
Alireza
Mirzaei
mirzaeialireza26@gmail.com
200319475328460066109
200319475328460066109
No
Bone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, Iran
Isa
Abdi Rad
isaabdirad@yahoo.com
200319475328460066110
200319475328460066110
No
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran
Rahim
Mahmodlou
mahmodlou@gmail.com
200319475328460066111
200319475328460066111
No
Department of Surgery, Imam Khomeini Hospital, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Fatemeh
Mansouri
mansouri1600@hotmail.com
200319475328460066112
200319475328460066112
No
Department of Genetics and Immunology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
Leili
Saeednejad Zanjani
200319475328460066113
200319475328460066113
No
Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran
Zeynab
Asadi- Lari
م
zeynab.asadilari@yahoo.co.uk
200319475328460066114
200319475328460066114
No
Department of Biology, Faculty of Science, University of Toronto, Toronto, Canada
Zahra
Madjd
majdjabari.z@iums.ac.ir
200319475328460066115
200319475328460066115
No
Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, & Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran