Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1397
11
1
gregorian
2019
2
1
33
1
online
1
fulltext
en
The bactericidal effect of liposomal vancomycin as a topical combating system against Methicillin-resistant Staphylococcus aureus skin wound infection in mice
Nanotechnology
Original Research
Original Research
<strong>Background: </strong>Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of skin infections and treatment is difficult due to its resistance to the most of antibiotics. Although vancomycin is often considered as an antibacterial agent of choice for the treatment of MRSA, its use is limited because of the high side effects. One solution is using liposomal formulation for local drug delivery. The aim of this study was to determine in vitro and in vivo efficacies of liposomal vancomycin as topical use.<br>
<strong> Methods: </strong>To prepare liposomal vancomycin, the ammonium sulfate gradient using remote loading and freeze-thaw methods was applied. Then, synthesized nanoliposomes were evaluated in terms of particle size, morphology, stability, and encapsulation efficiency. Minimum inhibitory concentration (MIC) of synthesized nanoliposome against MRSA was detected. The cytotoxicity of synthesized nanoliposome was evaluated using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Finally, the topical antibacterial activity of each formulation was tested against MRSA-infected skin wound model in mice.<br>
<strong> Results:</strong> High encapsulation efficiency was achieved for all synthesized nanoliposomes. The results of in vitro and in vivo showed that liposomal vancomycin was more effective than free vancomycin. Also, synthesized nanoliposome showed no cytotoxicity on human epidermoid cell line.<br>
<strong> Conclusion:</strong> The results showed that synthesized nanoliposome could be applied as a great topical antimicrobial construct for treatment of MRSA skin infections.<br>
<br>
MRSA, Antibacterial activity, Vancomycin, Liposome
941
947
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-5089-1&slc_lang=en&sid=1
Fahimeh
Hajiahmadi
fahime.hajiahmadi@gmail.com
200319475328460062171
200319475328460062171
No
Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran
Mohammad Yousef
Alikhani
alikhani43@yahoo.com
200319475328460062172
4029-4577-0003-0000
No
Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran
Hanifeh
Shariatifar
hanife1365@yahoo.com
200319475328460062173
200319475328460062173
No
Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran
Mohammad Reza
Arabestani
mohammad.arabestani@gmail.com
200319475328460062174
200319475328460062174
No
Department of Microbiology, & Brucellosis Research Center, Faculty of Medicine, Hamadan University of Medical Sciences, Hamedan, Iran
Davoud
Ahmadvand
d.ahmadvand@iums.ac.ir
200319475328460062175
200319475328460062175
Yes
Department of Medical Laboratory Sciences, Faculty of Allied Medicine, & Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran