Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1388
5
1
gregorian
2009
8
1
23
2
online
1
fulltext
en
A preliminary study of microsatellite instability analysis in different genotypes of p53 codon 72 in breast invasive ductal carcinomas
Anatomical Sciences
Anatomical Sciences
Original Research
Original Research
<p> <strong> Abstract </strong></p><p> <strong> Background: </strong>The polymorphic variants at codon 72 of the p53 gene, encoding either </p><p> proline or arginine at residue 72, produce marked change in the structure of p53. </p><p> From the evidence that the DNAmismatch repair system and p53 interact to maintain </p><p> genomic integrity, we hypothesized that the codon 72 variation may influence the </p><p> prevalence of microsatellite instability a feature of malignancies associated with </p><p> mismatch repair deficiency in breast invasive ductal carcinoma. </p><p> <strong> Methods: </strong>TP53 codon 72 genotypes were detected by PCR using specific primer </p><p> pairs for amplifying the Proline or the Arginine Alleles. Then, the frequencies of microsatellite instability (MSI) were analyzed in three genotypes of P53 codon 72 using </p><p> genomic DNAs from 120 specimens of breast ductal carcinomas by testing the </p><p> BAT-26 marker . </p><p> <strong> Results: </strong>From 120 specimens, 73(60.8%) was Arg/Arg, 31(25.8%) Arg/Pro and </p><p> 16(13.3%) Pro/Pro. MSI analysis revealed that 24.2% of the tumors (29 patients) was </p><p> microsatellite instability-positive and 75.8% (91 patients) was microsatellite instability </p><p> -negative. The frequency of microsatellite instability in the Arginine/Arginine, </p><p> Arginine/ Proline and Proline/Proline genotypes were 14 (19.2%), 12 (38.7%) and 3 </p><p> (18.8%) respectively. A significant difference in distribution of MSI was found for </p><p> the Arginine/ Proline genotype compared with (grouped) Arginine/Arginine and Proline/ </p><p> Proline genotypes (P=0.028). </p><p> <strong> Conclusion: </strong>Our findings suggested that breast invasive ductal carcinomas arising </p><p> in individuals with p53 codon 72 heterozygosity (Arginine/Proline) may be preferentially </p><p> prone to microsatellite instability more than other genotypes. </p><p> </p>
Microsatellite instability, polymorphism, P53, breast invasive ductal carcinoma
83
89
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-65&slc_lang=en&sid=1
Mehdi
Nikbahkt Dastjerdi
nikbakht@med.mui.ac.ir,
20031947532846001804
20031947532846001804
Yes
Dept. of Anatomical Sciences, Medical school, Isfahan University of Medical Sciences, Isfahan,Iran
Masoumeh
Faghani
20031947532846001805
20031947532846001805
No
Dept. of Anatomical Sciences, Medical school, Guilan University of Medical Sciences, Rasht, Iran
Mansour
Salehi
20031947532846001806
20031947532846001806
No
Dept. of Genetics, Medical school, Isfahan University of Medical Sciences
Mohammad
Rabbani
20031947532846001807
20031947532846001807
No
Dept. of Biotechnology, Pharmacy School, Isfahan University of Medical Sciences.