Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1380
8
1
gregorian
2001
11
1
15
3
online
1
fulltext
en
DETECTION AND RESTRICTION ANALYSIS OF C YTOMEGALOVIRUS DNA PERSISTING IN HUMAN ATHEROSCLEROTIC PLAQUES USING POLYMERASE CHAIN REACTION
Biological Sciences
Biological Sciences
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
The polymerase chain reaction (PCR) as applied to detection of a foreign
DNA in clinical specimens could provide a sensitive instrument for rapid detection
of viral DNA persisting in tissues of patients suspected of latent infection.
Human cytomegalovirus (HCMV) DNA was found in arterial plaques of patients
with atherosclerotic lesions using a PCR assay with nested primer oligonucleotides
derived from the major immediate early (MIE) exon 4 region of the genome.
In order to approach a possible part of HCMV DNA in the mechanism of
atherogenesis, the nature of found MIE exon 4 sequence was intimated using
restriction endonuclease mapping of the amplified DNA. Comparison of the restriction
fragments length polymorphism (RFLP) produced by endonuclease treatment
of viral DNA amplified from urine, blood, culture and arterial plaques displayed
a distinct difference in the DNA alignment for arterial specimens compared
to that of other sources. This approved the specific origin of the MIE DNA
found in plaques and suggested involvement in endothelial cell metabolism
changes. It could thus be established that PCR has exhibited the promise for investigation
of the role of latent viral infection in the process of atherosclerosis.
Human Cytomegalovirus, Atherosclerosis, PCR, RFLP.
155
159
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-253&slc_lang=en&sid=1
N
KHODAI BOORAN
20031947532846004148
20031947532846004148
Yes
From Pasteur Institute of Iran, Tehran, I. R. Iran
VV
BAKAYEV
20031947532846004149
20031947532846004149
No