Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1380
2
1
gregorian
2001
5
1
15
1
online
1
fulltext
en
APPLICATION OF DOT IMMUNOASSAY (DIA) FOR DETECTION AND TITRATION OF MEASLES VIRUS ANTIBODIES
General
General
Original Research
Original Research
Measles is one of the most contagious human diseases. Although mass vaccination
programs have reduced the incidence of this disease, measles is still an
important cause of morbidity and mortality among children in developing countries.
Therefore the use of sensitive techniques to evaluate vaccine efficacy and
level of immunity among members of susceptible communities is crucial. Serum
neutralization test (SNT) and Dot immunoassay (DIA) are among the best methods
utilized for evaluating measles virus antibodies. In this study, DIA was applied
for detection and titration of measles virus antibodies. This test was developed
for the first time in Iran in the Virology Department of the School of Medical
Sciences, Tarbiat Modarres University.
Viral antigen was first prepared and titrated. Then human IgG was isolated
by affinity chromatography. Anti-human immunoglobulin was prepared by immunizing
rabbits with human IgG and was later conjugated with peroxidase. DIA
was applied using these reagents.
The results indicated that the specificity and sensitivity of DIA in comparison
with SNT was 96% and 89%, respectively. This study demonstrated that DIA
is a rapid and simple test which can be applied for the detection of mass immunity
against the measles virus.
Measles virus, Serum neutralization test, DIA, Anti-human immunoglobulin.
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-276&slc_lang=en&sid=1
F
FOTOHI
20031947532846004231
20031947532846004231
Yes
From the Department of Virology, School of Medical Sciences, Tarbiat Modarres University, Tehran, Islamic Republic of Iran
MH
ROUSTAEE
20031947532846004232
20031947532846004232
No
H
SOLEIMANGAHI
20031947532846004233
20031947532846004233
No
AR
KHABIRI
20031947532846004234
20031947532846004234
No
M
MALEKANE
20031947532846004235
20031947532846004235
No
F
SABAHI
20031947532846004236
20031947532846004236
No