Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1380
2
1
gregorian
2001
5
1
15
1
online
1
fulltext
en
*Correspondence: M. Ghazi-Khansari. Ph.D., Department ofPharmacology,
Pharmacology
Pharmacology
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a by-product o f t he
trichlorophenol (herbicide) production and is also produced through a variety of
combustion processes. It appears that TCDD is a ubiquitous chemical, particularly
in industrialised countries, and the liver is the target organ of its toxicity. The
prominent hepatotoxic effect of TCDD is progressive centrilobular necrosis. The
isolated rat liver perfusion system approaches the normal physiology of the liver
and is ideal for studying biochemical alterations of the liver since hepatocytes are
easily exposed to various concentrations of chemicals with minimum neural-hormonal
effects. In this study, the liver was perfused with Krebs-Henseleit butfer
containing different concentrations of TCDD (0.3, 3, 20 and 30).lg/L). During the
perfusion many factors including gross liver appearance, bile formation, and aminotransferase
activities were assessed as indicators of liver viability. Consequently,
sections of liver tissue were examined for any histopathological changes. The
results showed that histopathological changes in liver tissues were related in a
dose-dependent manner to TCDD concentrations. In this instance doses of 20 and
30 ).lglL caused a significant (p<0.05) histopathological change in liver sections.
Liver necrosis and hemorrhage were also increased significantly in a dose-dependent
manner.
TCDD, Liver perfusion, Krebs-Henseleit buffer, Histopathological, necrosis.
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http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-280&slc_lang=en&sid=1
M
KARAMI
20031947532846004249
20031947532846004249
No
From the Department of Toxicology, School of Pharmacy, Tehran University of Medical Sciences
M
GHAZI-KHANSARI
ircongpp@nrcgeb.ac.ir
20031947532846004250
20031947532846004250
Yes
the Departments of Pharmacology School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. Iran
M
REZAYAT
20031947532846004251
20031947532846004251
No
the Departments of Pharmacology School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. Iran
B
MINAEI
20031947532846004252
20031947532846004252
No
the Departments of Histology, School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. Iran
M
ABDOLLAHI
20031947532846004253
20031947532846004253
No
From the Department of Toxicology, School of Pharmacy, Tehran University of Medical Sciences
O
SABZEVARI
20031947532846004254
20031947532846004254
No
From the Department of Toxicology, School of Pharmacy, Tehran University of Medical Sciences