Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1378
5
1
gregorian
1999
8
1
13
2
online
1
fulltext
en
MODULATION OF MITOCHONDRIAL UPTAKE OF A DENOSINE BY NITROBENZYLTHIOINOSINE
Pharmacology
Pharmacology
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
In this study the uptake and metabolism of adenosine by mitochondria has been
investigated. Incubation of CEM cells mitochondria preparation with [3H] -adenosine
showed substantial uptake and metabolism of adenosine. Adenosine was both
anabolized to AMP, ADP and ATP, and also catabolized to inosine. The highest
concentration of metabolites in extracted mitochondria was due to AMP. The
mitochondria preparation did not show any 5'-nucleotidase activity and this will
exclude any possibility of the production of adenosine from AMP in the
preparation. Coincubation of [3H]-adenosine with mitochondria in the presence of
2µM of a known potent nucleoside transporter inhibitor, nitrobenzylthioinosine
(NBMPR), substantially reduced the mitochondria content of adenosine and its
metabolites. The results of this study showed that adenosine was up taken by the
mitochondria preparation. Metabolism of adenosine after incubation with CEM
mitochondria provided further evidence for mitochondrial uptake and metabolism
of this nucleoside.
Adenosine, mitochondria, uptake, metabolism, nitrobenzylthioinosine (NBMPR).
124
127
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-406&slc_lang=en&sid=1
A. MAJID
CHERAGHALI
20031947532846004648
20031947532846004648
Yes
From the Department of Pharmacology and Toxicology, Faculty of Medicine, Baghyatollah University of Medical Sciences, Tehran, I.R. Iran
ALAN R.P
PATERSON
20031947532846004649
20031947532846004649
No
the Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada.
WENDY P.
GATI
20031947532846004650
20031947532846004650
No
the Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada.