Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1378
5
1
gregorian
1999
8
1
13
2
online
1
fulltext
en
EPINEPHRINE INHIBITS THE A CTIVITY OF PHOSPH ATIDATE PHOSPHOHYDROLASE OF ISOLATED HUMA N HEPATOCYTES
Clinical Biochemistry
Clinical Biochemistry
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
The effect of epinephrine on phosphatidate phosphohydrolase (PAP) activity of
isolated human hepatocytes was studied. Epineprine inhibited the enzyme activity
progressively at concentrations above 0.1 µM, reaching a maximum inhibition of
64.5% at 100 µM concentration. Inclusion of alprenolol, a β-receptor blocker, in
the incubation mixture abolished the inhibitory effect of epinephrine on PAP,
whereas the (i-receptor antagonist phentolamine, or agonist phenylephrine, did not
significantly change the hormone's effect. Addition of dibutyryl-cAMP or
.aminophylline (a cAMP phosphodiesterase inhibitor) to the incubation mixture
together with epinephrine caused further enzyme inhibiti.on reaching 65.6% and
63.7%, respectively, compared to 49% inhibition caused by epinephrine alone
under the same conditions. Dibutyryl-cAMP alone also inhibited PAP activity
(51 %). The results suggested that epinephrine affects human hepatocyte PAP
activity through β-adrenoceptor activation and cAMP is involved in the mechanism
by which PAP activity is altered.
Phosphatidate phosphohydrolase, Epinephrine, Adrenoceptors.
139
142
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-409&slc_lang=en&sid=1
BAHRAM
HAGHIGHI
20031947532846004656
20031947532846004656
Yes
From the Department of Clinical Biochemistry, Isfahan University of Medical Sciences, Isfahan, I.R.Iran
SHAHRAZ
TOORI
20031947532846004657
20031947532846004657
No
SHAHREBANOU
SHAJARIAN
20031947532846004658
20031947532846004658
No