Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1378
2
1
gregorian
1999
5
1
13
1
online
1
fulltext
en
COMPARISON OF FOUR PCR TESTS FOR THE DETECTION OF MYCOPLASMA PNEUMONIAE
Microbiology and Anatomy
Microbiology and Anatomy
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
Recently PCR is being used more frequently as a diagnostic method to detect M. pneumoniae. We used primer pairs reported by Van Kuppeveld,13 Leng,5 Lunerberg,7 an Bernet' targeting 16s rRNA, PI protein, tuf genes. and a short DNA equence (MP5) to evaluate the sensitivity among different PCRs. Reoptimization experiments showed that tuf PCR had the highest sensitivity amongst these four PCRs, detecting 10 organisms. Detection limit for the rest of the PCRs was 100 copies of DNA. This study confirmed that 92° would be the best dissociation temperature rather than higher temperatures that are still being used frequently in other studies. Be ides. accurate optimizing of the annealing temperature and extension time had important roles on the sensitivity as well as using milli-Q distilled water rather than double distilled water. Experiments done on MP5 PCR proved that the non- specific products mentioned in previous studie 2 were not eliminated by increasing the annealing temperature, although they disappeared on gel electrophoresis after careful optimization of extension time.
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79
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-427&slc_lang=en&sid=1
M
HAJlA
20031947532846005797
20031947532846005797
Yes
From the Department of Microbiology, Faculty of Medicine, Medical Sciences University of Hamadan, Hamadan. I.R. Iran
Dp
BACT
20031947532846005798
20031947532846005798
No
From the Department of Microbiology, Faculty of Medicine, Medical Sciences University of Hamadan, Hamadan. I.R. Iran
C.C.
STOREY
20031947532846005799
20031947532846005799
No
the Micrabiology Department. University of Leeds, Leeds. U.K.