Medical Journal of the Islamic Republic Of Iran
مجله پزشکی جمهوری اسلامی ایران
Med J Islam Repub Iran
Medical Sciences
http://mjiri.iums.ac.ir
2
journal2
1016-1430
2251-6840
8
10.18869/mjiri
14
8888
13
en
jalali
1377
11
1
gregorian
1999
2
1
12
4
online
1
fulltext
en
THE ROLE OF VARIOUS STABILIZERS IN THE ACQUISITION OF THERMO-TOLER ANCE IN FACTOR VIII ACTIVITY
Heart
Heart
Original Research: Basic Science in Medicine
Original Research: Basic Science in Medicine
We prepared a highly purified and relatively heat stable form of factor VIII
which contained 25 units per mL (u/mL) activity using PEG-4000 and developed an
effective and new manufacturing process. Heat treatment was performed at 80°C for
72 hrs in the presence of different stabilizers. In our studies, we used different organic
solvents as preservatives to maintain factor VIII activity, since few enzymes have
been observed to function perfectly well in such conditions. Our results show that the
activity of factor VIII in the presence of organic solvent at 80°C for 72 hrs as viral
inactivation is significantly decreased. It seems that irreversible aggregation of
protein is the major mechanism in cessation of factor VIII activity. On the other hand,
protein stability may be increased by certain low molecular weight substances. We
therefore used different sugars and amino-acids to protect factor VIII activity in the
process of heat treatment. Our results showed that glycine, glucose, saccharose,
mannitol and BSA were not effective stabilizers of factor VIII. But the loss of factor
VIII activity at 80°C for 72 hrs, in the presence of 10, 15 and 20% concentrations of
trehalose, was about 15-20%, which is acceptable for large-scale processes. Thus, the
condition obtained in the present study seems to be a more suitable condition than
that reported previously.
393
397
http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-445&slc_lang=en&sid=1
T
ZANDIEH
20031947532846004747
20031947532846004747
Yes
From the Iranian Blood Fractionation and Research Company, Tehran
M
FARHADI
20031947532846004748
20031947532846004748
No
the Iranian Blood Transfusion Service, Tehran
M
SHAHR-ABADI
20031947532846004749
20031947532846004749
No
the Virology Lab, Rasool Akram Hospital, Tehran, I.R. Iran.
H
LOTFI
20031947532846004750
20031947532846004750
No
From the Iranian Blood Fractionation and Research Company, Tehran