eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
243
246
article
IDENTIFICATION OF LEISHMANIA SPECIES FROM DIFFERENT PARTS OF IRAN USING A RANDOM AMPLIFIED POLYMORPHIC DNA IN HUMANS, ANIMAL RESERVOIRS AND VECTORS
M MOHEBALI
1
MH MOTAZEDIAN
2
F PARSA
3
H HAJJARAN
4
MR YAGHOOBI-ERSHADI
5
From the School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran
the Medical Faculty. Shiraz University of Medical Sciences, Shiraz,IR. Iran.
In this study, we used Random Amplified Polymorphic DNA (RAPD) for
identification of 17 isolates of Leishmania from the skin and reticuloendothelial
system of humans, animal reservoirs (rodent and dog) and sandflies in various
parts of Iran in the last decade.
Fifteen species have been confirmed by isoenzyme characterization by the
London School of Hygiene and Tropical Medicine and Shiraz University of Medical
Sciences. In this study, strong confirmation has been observed between random
amplified polymorphic DNA with isoenzyme characterization.
http://mjiri.iums.ac.ir/article-1-790-en.pdf
Leishmania species
RAPD-PCR
Iran.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
237
242
article
MONOCLONAL ANTIBODIES TO HUMAN ALPHA-FETOPROTEIN
Z AMIRGHOFRAN
amirghz@sums.ac.ir
1
AK SHEIKHI
2
From the Department of Immunology, Shiraz Univeristy of Medical Sciences, Shiraz, I.R. Iran.
Alpha-fetoprotein (AFP), a serum glycoprotein belonging to the onco-developmental
proteins group, serves as a marker both in cancer research and in studies
concerning fetal development and fetal pathophysiology.
Monoclonal anti-AFP antibodies are essential reagents in developing appropriate
techniques for measurement of this protein. In this study, in order to produce
anti-AFP monoclonal antibody (mAb), AFP was partially purified from cord
sera using two-step ion-exchange chromatography on DEAE-cellulose with a final
recovery of about 570µg. MAbs against this preparation was raised by hybridoma
technology using Ag8.653 mouse myeloma cells as the fusion partner. Hybridomas
appeared in 10% (30/300) of culture wells and of these 2 clones were
found to be positive for anti-AFP production. In western blot analysis a 70 kD
band from dead fetus serum-but not adult serum-was stained by both mAbs. A
sandwich ELISA technique using polyclonal antisera on one side and mAbs on
the other side was employed to plot dose response curves. The positive dose dependent
reactivity of the mAbs with standard AFP and other AFP containing
samples and the negative reaction with normal adult sera lacking AFP showed the
specificity of the mAbs for AFP.
http://mjiri.iums.ac.ir/article-1-789-en.pdf
Monoclonal antibody
Alpha-fetoprotein
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
231
236
article
FORMALIN AS A PERIPHERAL NOXIOUS STIMULUS CAUSES A BIPHASIC RESPONSE IN NUCLEUS PARAGIGANTOCELLULARIS NEURONS
E SOLEIMAN-NEJAD
1
Y FATHOLLAHI
2
S SEMNANIAN
3
From the Dept. of Physiology. Faculty of Medicine. Army University of Medical Sciences. Po. Box: 14185- 611. Tehran
The Dept. of Physiology. School a/Medical Sciences. Tarbiat Modarres University. Po. Box: 14115-111. Tehran.
the Institute of Biochemistry and Biophysics. Tehran University. Po.Box: 13145-1384. Tehran.l.R. Iran.
The effects of formalin as a peripheral noxious stimulus on the activity of
lateral paragigantocellularis nucleus (LPGi) neurons were examined. Spontaneous
activity ofLPGi neurons was recorded after confirmation of their responsiveness
to acute pain, and thereafter formalin (50 µL, 2.5%) was injected in the
contralateral hindpaw. The response of the LPGi neurons was monitored for 60
min. A biphasic response with a peak lasting 3 to 5 min post-injection, and a
second more prolonged tonic excitatory response were obtained which corresponds
to the nature and time course of behavioral studies. It is concluded that LPGi
neurons may be involved in the processing of nociceptive information related to
formalin as a noxious stimulus.
http://mjiri.iums.ac.ir/article-1-788-en.pdf
Nucleus paragigantocellularis
Formalin test
Multiple unit recording
Rat.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
227
230
article
P REPARATION OF A NEW MEDIUM FOR IN VITRO CULTURE OF BORRELIA MICROTTI AND BORRELIA PERSICA
MEHDI ASSMAR
1
MOHAMMAD SOLEIMANI
2
NOORAIR PIYAZAK
3
FARZAD OREIZI
4
SEYED HOSSEIN HOSSEINI
5
ZAHRA ZAMANI
Zamani@institute.pasteur.ac.ir
6
From the Departments of Parasitology institute 0f Iran, Tehran, l.R. Iran.
*From the Departments of Parasitology, and Biochemistry, Pasteur institute 0f Iran, Tehran, l.R. Iran.
Borrelia microtti and Borrelia persica are two Iranian strains of spirochetes
whose vectors are Ornithodorous tholozani and Ornithodorous erraticus which
are responsible for relapsing fever. BSK medium has been used for in vitro culture
of other strains of borrelia, although the two mentioned strains could not be
successfully cultured in it. We have modified BSK medium by using guinea pig
and fetal calf serum instead of rabbit serum. It was observed that for in vitro
culture of Borrelia persica, guinea pig serum and for Borrelia microtti, fetal calf
serum show the best growth.
http://mjiri.iums.ac.ir/article-1-787-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
219
225
article
TRANSFORMING GROWTH FACTOR β2 UP-REGULATES GM-CSF GENE IN HUMAN BLADDER CARCINOMA CELL LINE HTB 5637
Z SOHEILI
1
B GOLIAEI
goliaei@ibb.ut.ac.ir
2
From the Laboratory of Biophysics and Molecular Biology, Institute 0f Biochemistry and Biophysics, University of Tehran, Tehran, I.R. Iran.
Transforming growth factor betas are multifunctional polypeptides in the
cytokine superfamily. They have a growth inhibitory role on hemopoietic progenitor
cells in semisolid colony assay as well as in long-term bone-marrow culture.
TGF - β2 represses stromal cells, stem cell factor gene transcription, and decreases
the stability of c-kit transcripts in hemopoietic cells. TGF-β also modulates
GM-CSF production from human lymphocytes. The present study reveals
the TGF- β2 role in production of GM-CSF in HTB 5637, human bladder carcinoma
cell line. HTB 5637 cells were treated with 5 ng/mL of human TGF - β2'
viable cells were counted and GM-CSF concentration was determined, No antiproliferate
activity of TGF- β2on HTB 5637 cell line was observed. Biological
assay showed increased levels of GM-CSF in the supernatant of cultured cells.
However this increase was lower than that expected from ELISA. Since TGF- β
may be an active suppressor factor regulating hemopoiesis, it seems that some
inhibitory factor(s) may be produced (increased) in response to TGF- β2 treatment.
It has been shown that GM-CSF mRNA content from HTB 5637 cell line
is very stable and this stabilization is translational dependent. Using Slot blot and
Northern blot analysis, we determined that TGF- β2 upregulated GM-CSF gene
expression in HTB 5637 cell line. The results suggest that TGF- β2 upregulates
the production of GM-CSF gene at the transcriptional level.
http://mjiri.iums.ac.ir/article-1-786-en.pdf
TGF-β
GM-CSF
HTB-5637
Bladder carcinoma.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
209
217
article
HEPATOMA McARDLE-RH7777 CELLS HAVE THE SAME RESPONSE AS NORMAL RAT HEPATOCYTES TO BOTH DIBUTYRYL-cAMP AND ANTICALMODULINW-7
M RASOULI
mehdi.rasouli@planetaccess.com
1
R LEHNER
richard.lehner@ualbera.ca
2
From the Department of Biochemistry and Lipid & Lipoprotein Research Group, University of Alberta, Edmonton, Canada T6H 2S2.
The effects of cAMP-analogue dibutyryl-cAMP and anticalmodulin W-7 were
studied on de novo synthesis and secretion of lipids in cultures of hepatoma McArdle
RH7777 cells and normal rat hepatocytes. Dibutyryl-cAMP and W -7 separately
caused a significant decrease in the secretion of de novo synthesized triacyl
[3H]glycerol in both cultures of McArdle cells and rat hepatocytes. The inhibitory
effects of dibutyryl-cAMP and W-7 were concentration-dependent and appeared
at the lowest concentration examined, 5JlM and 20JlM respectively. DibutyrylcAMP
at a concentration of 50 JlM and W -7 (20 µM) suppressed the secretion of
triacylglycerol by approx. 38% (p<0.05) and 37% (p<0.05) respectively. Dibutyryl.cAMP
but not W -7 also suppressed the secretion of phosphatidylcholine significantly.
Dibutyryl-cAMP and W-7 had no significant effect upon [3H]glycerollabeled
de novo formed triacylglycerol and phosphatidylcholine, except at the
highest concentration tested, 500JlM and 50JlM respectively, where both
triacylglycerol and phosphatidylcholine synthesis were suppressed significantly.
Similar findings were obtained on cultured hepatocytes.
The molar ratios of newly made triacylglycerol/phosphatidyl choline in the
media and cells of hepatocytes were about four times compared to that of McArdle
cells, indicating more lipidation and core expansion of nascent lipoprotein particles
in hepatocytes. The molar ratio of triacylglycerol/phosphatidyl choline in
the medium and cells of cultured McArdle cells was unchanged significantly i n
the presence of either dibutyryl-cAMP or W-7. However, the molar ratio was
decreased 25% (p<O.Ol) in the presence of dibutyryl-cAMP in the media but not
in the cells of cultured hepatocytes. These results suggest that hepatoma McArdle
cell's response to both cAMP-analogue and calmodulin antagonist are comparable
to that of normal rat hepatocytes. In addition, the inhibitory effects of
dibutyryl-cAMP and W-7 at low concentration are unlikely to be due to the suppression
oftriacylglycerol and phosphatidyl choline synthesis.
http://mjiri.iums.ac.ir/article-1-785-en.pdf
Calmodulin
Cyclic AMP
Hepatocyte
McArdle cells and VLDL
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
203
207
article
ANTIBODY TO MITOCHONDRIAL COMPLEX-I IN SOME PATIENTS WITH MULTIPLE SCLEROSIS
MH SANATI
1
PR CARNEGIE
2
From the National Research Center for Genetic Engineering and Biotechnology, Tehran, I. R. Iran
the Biotechnology Research Grpup, Murdoch University, Western Australia 6150, Australia
When pooled immunoglobulin G (IgG) from patients with multiple sclerosis
(MS) was used to probe a human fetal spinal cord λgt 11 cDNA library, the IgG
was found to bind to a predicted epitope of human mitochondrial ND4 sequence.
To investigate the involvement of the ND4 as an autoantigen in MS, we determined
the presence of specific antibody in the serum of MS patients and serum
samples of some other autoimmune disease as controls.
A peptide, which is part of the ND4 protein in human mitochondrial complex
I, CysLeuAlaAsnSerAsnTyrGluArgThrHisSerArg, was conjugated with a
maleimido-thiol bond to diphtheria toxoid and used as an autoantigen. To remove
any IgG which bound to diphtheria toxoid and the bovine serum albumin (BSA)
blocking agent in the ELISA, the sera were preadsorbed before being incubated
with the conjugate. About 20% of patients with multiple sclerosis (MS) had antibody
to the peptide and when present, the level was found to fluctuate. In preliminary
experiments autoantibody to ND4 was found to be not specifically associated
with MS. The prevalence and involvement of the autoantibody in multiple
sclerosis remains to be determined.
http://mjiri.iums.ac.ir/article-1-784-en.pdf
Multiple sclerosis; Autoantibody
Mitochondria.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
199
202
article
PREVALENCE OF HYPERCALCEMIA AND HYPERCALCIURIA IN THYROTOXIC PATIENTS OF A REFERRAL ENDOCRINE CLINIC
S KALANTARY
1
Many patients with thyrotoxicosis develop hypercalciuria and nypercalcemia.
Urine calcium excretion depends on diet, latitude and sunshine exposure.
With regard to variation of these indices in different geographical regions, we
conducted a one-year study on 202 patients, 65 males and 137 females, with thyrotoxicosis
of varying severity. The mean age of patients was 35.24±11.95 and
35.87±12.21 years for men and women respectively. Free T4 index, serum calcium
and phosphorus, PTH and alkaline phosphatase were measured in all patients.
The mean for FTI was 7.48±1.98. Serum Ca and P mean was 9.31±0.46
mg/dL and 4.11±0.61 mg/dL respectively. 3 patients (1.5%) had hypercalcemia
and hyperphosphatemia was detected in 38(18.6%) patients.
The mean of urine Ca and P were 190±106.7 mg/24h and 626±246.73 mg/
24h respectively. Increased urine Ca and P was detected in 20(9.8%) and 4(4.4%)
patients respectively. PTH was suppressed in 34(16%) and alkaline phosphatase
was increased in 50(24.5%) patients.
There was no significant relation between FTI with serum and urine Ca and
P and serum PTH and alkaline phosphatase in thyrotoxic patients. There was also
no appreciable difference in serum Ca and P and urine Ca between the two sexes
with respect to the lower exposure of muslim women to sunlight.
These results contrast with the high frequency of hypercalcemia (27%) and
hypercalciuria in patients with thyrotoxicosis in previous studies and might be
due to habitual low calcium diet and sunshine exposure in this area.
http://mjiri.iums.ac.ir/article-1-783-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
195
198
article
CARDIAC SEQUELAE OF KAWASAKI DISEASE IN ISFAHAN, IRAN
AR NIKYAR
1
R KORDYDARIAN
2
R lRANPOUR
3
From the Department of Pediatrics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Islamic Republic of Iran.
Kawasaki disease is one of the major causes of acquired heart disease of
children in developed countries. This study was performed to determine the frequency
of cardiac sequelae of kawasaki disease and related risk factors based on
a descriptive cross-sectional study between 1994-1999 in the pediatric ward of
AI-Zahra Hospital of Isfahan.
Out of 45 patients, 29 were male (64.4%) and 16 were female (35.6%) with
a sex ratio of 1.8 (p<0.05). Five patients suffered from pericardial effusion (11.1 %),
6 patients had mitral valve insufficiency (13.3%) and 10 patients (22.6%) had
coronary artery aneurysms (CAA). Seven and 3 cases of these 10 CAA cases were
male and female, respectively, with a sex ratio of 2.3 (p<0.05). CAA's were less
in patients who received intravenous immunoglobulin (IVIG) in the first 10 days
than untreated cases or cases who were treated later (p<0.05). CAA's persisted
only in 2 patients after the convalescent period of disease and a new case of CAA
which did not exist in the acute phase was detected. In conclusion, CAA's in this
study were higher than worldwide reports. Early diagnosis and treatment with
IVIG are necessary for prevention of cardiac involvement in kawasaki disease
http://mjiri.iums.ac.ir/article-1-782-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
189
193
article
PEDICLE MUSCLE FLAPS IN IRRADIATED WOUNDS: DOES PREVIOUS RADIATION OF THE MUSCLE TO BE TRANSPOSED-AFFECT THE OUTCOME? A 9 YEARS EXPERIENCE OVER 206 CONSECUTIVE CASES
HA NIKPOUR
1
From the Department of Plastic and Reconstructive Surgery, Shiraz University of Medical Sciences, Shiraz, I.R. Iran.
Radiation-related wounds challenge surgeons in all disciplines of surgery.
Wound-healing complications are commonplace, and solutions for reconstruction
are limited. Muscle and musculocutaneous flaps have improved this situation.
But the question is, does previous radiation of the muscle to be transposed
affect the outcome?
143 consecutive previously irradiated patients treated with muscle or musculocutaneous
flaps composed the group under consideration: these 143 patients
had 206 muscles transposed. The overall complication rate for muscle transposition
to close a radiated wound was 20 percent.
Of the 143 patients who received radiation, 62 patients had the muscle transposed
for wound closure from the primary field of radiation. 81 patients were
closed with non-irradiated muscle. When the transposed muscle had been radiated,
the complication rate was 29.6 percent in 14.3 percent, the entire muscle
underwent necrosis, requiring total removal and a second tissue transposition from
a non-irradiated source to achieve closure. The subgroup using non-irradiated
muscle had a complication rate of 12.2 percent 1 patient in this group had complete
flap necrosis requiring a second tissue transposition. No postoperative deaths
were encountered.
The experience in our department reveals that non-irradiated muscle is the
best choice for closure of a radiated wound, if possible.
http://mjiri.iums.ac.ir/article-1-781-en.pdf
Irradiation
Muscle flap
Muscle transposition.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
2002-02
15
4
183
187
article
EF FECTS OF THE VALSALVA MANEUVER AND HEAD ROTATION ON INTERNAL JUGULAR VEIN DIAMETER AND LOCATION BY ULTRASONOGRAPHY
MH BAGHERI
1
H KAMALIPOUR
Kamalih@sums.ac.ir
2
M JAVDANI
3
From the Department of Anesthesiology. Shiraz University of Medical Sciences. Shiraz. l.R. Iran
Department of Anesthesiology, Faghihi Hospital, Zip code 71344, Shiraz,I.R.Iran
In order to determine the effect of Valsalva maneuver and head rotation on
the internal jugular vein (IJV) diameter and cross-sectional area and overlap with
the carotid artery (CA) and find the best technique for safe cannulation of the IJV
and decrease the risk of CA puncture, the diameter of both the IJV and the CA
and percentage of overlap between the two vessels and cross-sectional area of the
IJV were measured by ultrasonography on the right side of the neck in the supine,
head down position, at three different degrees of head rotation with and without
the Valsalva maneuver in 30 subjects. The results were analyzed by two-way repeated-
measures analysis of variance followed by least significant difference
(LSD).
Head rotation increased the overlap between the two vessels (p<0.001).
Valsalva's maneuver also increased overlap between the twp vessels (p<0.02)
and increased IJV diameter and cross-sectional area (p0.05). Valsalva's
maneuver and head rotation did not change CA diameter significantly (p>0.05).
We therefore advocate the neutral head position with Valsalva's maneuver
as a safe and reliable method for IJV cannulation.
http://mjiri.iums.ac.ir/article-1-780-en.pdf
Valsalva\'s maneuver; Jugular vein
internal; Catheterization
central venous; Head movements