eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
237
240
article
TRACHEOBRONCHOPATHIA OSTEOCHONDROPLASTICA
EBRAHIM RAZI
1
TAHEREH KHAMEHCHIAN
2
From the Department of Internal Medicine, Kashan University of Medical Sciences, Kashan, Islamic Republic of Iran.
Tracheobronchopathia osteochondroplastica (TO) is a rare and usually benign
disorder affecting the trachea and occasionally the bronchi.
A case of TO was diagnosed in a 61 year old male farmer presenting with
pulmonary infections and prolonged productive cough. A bronchoscopy revealed
multiple nodular excrescences aong the anterolateral wall of the trachea and main
bronchi. CT scans showed moderate narrowing and distortion of the trachea.
Biopsies revealed squamous metaplasia, and calcification and ossification in the
epithelial surface. The literature on the subject is reviewed here. The severity of
TO ranges from no symptoms to severe dyspnea, hemoptysis or pneumonitis. The
etiology and pathogenesis is unknown. Treatment is seldom necessary. Awareness
of the condition as a differential diagnosis to neoplasms is important to avoid
unnecessary surgery or chemotherapy.
http://mjiri.iums.ac.ir/article-1-944-en.pdf
Tracheobronchopathia osteochondroplastica
Fiberoptic bronchoscopy
Computed tomography
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
235
236
article
DOPA RESPONSIVE DYSTONIA: A CASE REPORT
MOHAMMAD GHOFRANI
1
HASSAN TONEKABONI
2
From the Department of Pediatric Neurology, Mofid Children's Hospital, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran
Dopa responsive dystonia (DRD) is a dystonic syndrome of childhood,
usually affecting gait and subsequently developing into parkinsonism with a
dramatic therapeutic response to levodopa.
At the best of our recollection this is the first case in Iranian medical literature
of a 13 year old boy, affected by this interesting, rare and treatable disease which
can be easily diagnosed if clinicians have a high index of suspicion.
We also have a brief discussion on the pathophysiology of DRD.
http://mjiri.iums.ac.ir/article-1-943-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
231
234
article
PULMONARY BLASTOMA: REPORT OF A CASE AND REVIEW OF THE LITERATURE
MOHAMAD REZA DAVOUDI
1
DONIA FARROKH
2
From the Department of Radiology, Imam Reza Medical Center, Mashhad University of Medical Sciences, Mashhad, Islamic Republic of Iran.
We describe a case of pulmonary blastoma, a rare primary lung malignancy
which affects a younger population. This tumor contains both mesenchymal and
epithelial elements. Pulmonary blastoma tends to relapse locally and metastasizes
mainly in the first few years. It has a poor 5-year prognosis, and shows a potentid
radio-chemosensitivity. Because these tumors are considered malignant, surgical
excision is recommended.
In this paper, we describe a case of pulmonary blastoma in a 3 year old boy with
a brief review of literature.
http://mjiri.iums.ac.ir/article-1-942-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
225
230
article
IMMUNOSUPPRESSION IN HUMAN UTERINE TISSUES THROUGHOUT THE REPRODUCTIVE CYCLE
MEHRI GHAFOURIAN BOROUJERDNIA
1
RAHIM CHINIPARDAZ
2
From the Department of Immunology, Medical College, Ahwaz University of Medical Sciences
the *Department of Statistics, College of Sciences, Shahid Chamran University, Ahwaz, Islamic Republic of Iran.
The purpose of this study was to investigate and compare whether the explant
supernatants prepared from endometrial tissues during the proliferative phase,
secretory phase and early pregnancy differ in immunosuppressive activity.
Specimens of nonpregnant endometrium from hysterectomies and normal first
trimester decidua following elective aspiration termination of pregnancy were
obtained. Supernatants from culture of endometrium or decidual fragments were
prepared after 24, 48 and 72 hours. The effect of the supernatants on mitogeninduced
lymphoproliferation in vitro was assessed. The percentage of suppression
was calculated and then data were evaluated using Student's t-test. The results
confinned that endometrial immunosuppressive activity exists throughout the
reproductive cycle and confirm that early pregnancy decidua is a source of
immunoregulatory factors. Immunosuppressive activity was increased in early
pregnancy decidua compared with secretory phase endometrium but the results did
not reach statistical signifIcance. In contrast, the difference in immunosuppressive
activity between proliferative endometrium and early pregnancy decidua was
highly significant. In conclusion, the high frequency of early embryonic loss
before implantation and the low success rate of in vitro fertilization (IVF), embryo
transfer and pathological pregnancy in humans can be explained in part by lack of
suppressor activity in the endometrium.
http://mjiri.iums.ac.ir/article-1-941-en.pdf
Immunosuppression
Pregnancy
Endometrium
Menstrual cycle.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
219
223
article
INTRACYTOPLASMIC SPERM INJECTION (lCSI) IN MICE BY A NEW HOLDING PIPETTE
AMIR ESMAILNEJAD MOGHADDAM
1
ABBASALI KARIMPOUR MALEKSHAH
2
MASAHIKO HIROI
3
HIDEKAZU SAITO
4
From the Department of Anatomy and Embryology, Medical School, Mazandaran University of Medical Sciences, Sari, I R. Iran
From the Department of Anatomy and Embryology, Medical School, Mazandaran University of Medical Sciences, Sari, I R. Iran
the Department of Obstetrics and Gynecology, Medical School, Yamagata University, Yamagata, Japan.
the Department of Obstetrics and Gynecology, Medical School, Yamagata University, Yamagata, Japan.
Mice are ideal models for ICSI experiments because of the ease of culturing
eggs/embryos in vitro and the availability of ample genetic information.
Unfortunately, it has been extremely difficult. In this study we improved the mouse
ICSI method by using a new holding pipette that was made of two pipettes such
that one pipette was pulled and heat merged into the other one. The outer pipette
had an outer diameter of 120 J..l. and an inner diameter of 80 J..l. to 85 J.1 (about mouse
oocyte diameter).
The inner pipette had an outer diameter of 80 µ to 85 µ. and an inner diameter of
55 µ to 60 µ. which was polished and narrowed to 20 J..l.on amicroforge. The distance
between the tips of the two pipettes was adjusted to 120 µ. to 160 µ (1.5 to 2 fold
longer than mouse oocyte diameter). Of 307 oocytes which were injected with a
single spermatozoon, 206 (67.1 %) survived and 93 (45.1 %) of surviving oocytes
showed normal fertilization (2 pronuclei and second polar body). Of 109 oocytes
which were only sucked into the holding pipette (control group), 105 (96.3%)
survived and only 4 (3.8%) of them became activated parthenogenically.
U sing this new holding pipette, the oocyte is sucked into a glass tunnel and
elongates to a reasonable length therefore the injection axis will be increased and
piercing of the oolemma can be performed easily.
http://mjiri.iums.ac.ir/article-1-940-en.pdf
Intracytoplasmic spenn injection
mouse ICSI
holding pipette
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
213
217
article
ROLE OF CYTOSOLIC GL U TATHIONE S- T RANSFERASES IN PRO TEC TION AGAINS T ACE TAMINOPHEN-INDUCED LIPID PEROXIDATION IN WEANLING RATS
A A. ALLAMEH
1
M NIKSERESHT
2
F KHEYRDOOSH
3
From the Department of Biochemistry, Faculty of Medical Sciences, Tarbiat Modaress University, p.o. Box 141554838, Tehran,I.R.Iran.
Resistance of the weanling rat to acetaminophen (AP AP)-induced hepatotoxicity
is manifested with regard to a surge in APAP-glutathione (OSH) conjugate
formation in the liver [Allameh et al. Mech Aging Dev 95(1997)71]. The present
study was conducted to assess the role of this detoxification pathway in APAPinduced
lipid per6xidationin the liver. Lipid peroxidation measured as thiobarbituric
acid reactive substances (TBARS) in rat liver homogenate was observed to be
increased due to a decrease in hepatic cellular GSH concentration. Cellular GSH
content was relatively lower in growing liver and further decreased in rats treated
with either GSH-depleting agents or APAP, whereas adult animals under APAP
treatment suffered significantly less depletion of GSH. AP AP injection to weanling
rats pre-treated with diethylmaleate (DEM) aggravated lipid peroxidation.
Administration of a single large dose of APAP (500 mg/kg b. w.) to weanling rats,
3 h before sacrifice, which caused 46% GSH depletion, resulted in a 25% increase
in lipid peroxidation. Pre-treatment of growing rats with DEM, 30 min before
APAP, caused about 70% depletion in GSH content as a result of which there was
a further increase (approx. 1.6 fold) in lipid peroxide formation (control: 37.40
experimental: 60.76 nmol malondialdehyde formation/g tissue). GSH S-transferase
activity is not necessarily a determinant of APAP toxicity in adult animals. Unlike
adults, in growing tissues the enzyme activity is indu.ced single overdose of APAP. When these data are discussed in relation to our earlier
study, it could be concluded that APAP-dependent induction of GSHS-transferases
is responsible for increased APAP-GSH conjugate formation which facilitates
inactivation of NAPQI as well as other toxic metabolites of lipid peroxidation.
http://mjiri.iums.ac.ir/article-1-939-en.pdf
Acetaminophen
hepatotoxicity
lipid peroxidation
weanling rat
glutathione
glutathione Stransferase.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
207
211
article
FIVE ALPHA DIHYDROTESTOSTERONE (5α-DHT) MAY MODULATE NITRIC OXIDE RELEASE VIA ENDOGENOU S CYTOKINES IN PERITONEAL MA CROPHA GES OF NZB/BALBc MICE
KAZEM AHMADI-RENANI
1
ALAN B. McCRUDEN
2
From the Department of Immunology, Faculty of Medicine, Baghiatollah University of Medical Sciences,
the Department of Immunology, University ofStrathclyde, Glasgow, G4
Recent studies have established that sex hormones directly or indirectly affect
T and B cells and macrophages by manipulating the production of cytokines. In this
study the possibility of the effect of 5a-DHT on macrophage (MΦ) nitric oxide
(NO) release via interleukin-l, 6 (lL-1β, IL-6) or tumor necrosis factor-a (TNFα)
was investigated. The endogenous cytokines IL-1β, IL-6 and TNF-α were
neutralized by hamster anti-mouse IL-1β, rat anti-mouse IL-6 or goat anti-mouse
TNF-α monoclonal antibody, respectively. Blocking of IL-1β and TNF-α resulted in decrease in NO release. Neutralizing of IL-6 caused an increase in nitric
oxide (NO) production. With regard to these findings, it can be concluded that 5αDHT
may enhance NO production in peritoneal macrophages via modulation of
cytokine secretion.
http://mjiri.iums.ac.ir/article-1-938-en.pdf
Nitric oxide
Cytokine
Testosterone.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
195
206
article
SENSORIMOTOR CONTROL OVER FUSIMOTOR NEURONS OF THE TENUISSIMUS MUSCLE IN THE A NESTHETIZED CAT: A QUALITATIVE PRIMARY AFFERENT RECORDING
ALlREZA ASGARI
1
From the Department of Physiology and Biophysics. Baghiyatollah (a.s.) University of Medical Sciences. Tehran. I. R. Iran
Cortical control of the sensory output of muscle spindles was studied in
thirteen anesthetized cats in the present experiment. Gamma motoneuron activity
was monitored during electrical stimulation of the sensorimotor cortex while
recording from single primary afferents from the tenuissimus muscle. Findings are
as follows:
1. The state of anesthesia is crucial in obtaining reproducible results and
variation in the state of anesthesia can alter the fusimotor effect from static to
dynamic or even from excitation to inhibition, a finding consistent with those of
Vedel and Mouillac-Baudevin.30The anesthetic agent used was also important in
determining the magnitude and types of responses to corticaI stimulation. the
initial burst of the primary afferent in response to passive stretch was by far greater
with chloralose than with barbiturate anesthesia in the tenuissimus muscle,
suggesting that there may be a tonic low-level dynamic gamma excitation in
chloralose anesthesia.
2. The state of the sensorimotor cortex is another determinant factor. Prevention
of CO2 escape from the surface of the cortex in the present experiments, by
covering the cortex with 1cm of mineral oil, is thought to be the sole factor which
made these results different from those obtained by Gladden and McWilliam.ll•12
3. Different types of static gamma motoneurons could be recruited from the
sensorimotor cortex independently.
4. The topographical mapping of the sensorimotor cortex in relation to the
type of recruited gamma motoneurons, static or dynamic, was as follows: a)
A"dynamic area" was identified from which dynamic effects were clearly elicited
during stimulation. b) Static effects were elicited following stimulation of a much
wider area across the sensorimotor cortex, the postcruciate dimple being almost at
the center.
5. The sensorimotor cortex was not only capable of controlling static gamma
motoneurons independently from dynamic ones, but also capable of simultaneously
inhibiting static gamma motoneurons and exciting others, lending support to the
idea put forward by others.6
http://mjiri.iums.ac.ir/article-1-937-en.pdf
Fusimotor
Gamma motoneuron
Sensorimotor
Primary afferent
Tenuissimus.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
185
189
article
RENAL ALLOGRAFT ACCUMULATION OF TECHNETIUM-99M SULFUR COLLOID AS A PREDICTOR OF GRAFT REJECTION
BEHZAD EINOLLAHI
1
MOHAMMADREZA KHATAMI
2
MAHBOOB LESANPEZESHKI
3
PEJMAN BAKHTIARI
4
VIOLET AMIRJALALI
5
AHMAD FIROUZAN
6
From the Department of Nephrology, Baghiyatollah Hospital, Baghiyatollah University of Medical Sciences
the Department of Nephrology, Imam Khomeini Hospital, Tehran University of Medical Sciences,
the Department of Nephrology, Imam Khomeini Hospital, Tehran University of Medical Sciences,
From the Department of Nephrology, Baghiyatollah Hospital, Baghiyatollah University of Medical Sciences
the Department of Nuclear Medicine, Shahid Labafinejad Hospital, Shahid Beheshti University of Medical Sciences
the Department of Nephrology, Azad Islami University of Medical Sciences, Tehran, I. R. Iran.
A perplexing issue in diagnosing the cause of renal allograft dysfunction is
differentiation between rejection----the most common cause--and many other
possibilities that have detrimental effects on graft function. This study was
designed to determine whether technetium - 99m sulfur colloid (TSC) accumulation
could predict graft rejection. We prospectively studied 54 episodes of allograft
dysfunction in 53 kidney transplant recipients who had undergone TSC
scintiscanning and graft biopsy, within one week of evidence of allograft
dysfunction.
Visual analysis of TSC uptake was done by comparing allograft uptake with
that of the fifth lumbar vertebra (L5) marrow. A 3+ result meant that allograft
uptake was greater than L5 marrow uptake 2+, allograft uptake was the same as
L5 marrow uptake 1+, less than and 0, no allograft uptake. Transplant accumulation
of 2+ was considered consistent with rejection (p=0.01). Allotransplant biopsies
were interpreted based on the Banff Working Classification and rejection was
noted in 45 of 54 renal biopsies. 42 of 45 biopsy -proven rejection episodes had ≥2+
graft uptake.
This nuclear medicine technique has a sensitivity of 93.3%, specificity of
44.4%, a positive predictive value of 89.3%, a negative value of 57.1 % and an
efficiency of 83.3% in the diagnosis of renal allograft rejection.
http://mjiri.iums.ac.ir/article-1-935-en.pdf
Kidney transplantation
Allograft dysfunction
Rejection
Technetium-99m sulfur colloid.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
179
183
article
MODIFIED RADICAL MASTOIDECTOMY: TECHNIQUES TO DECREASE FAILURE
MOHAMMAD AJALLOUEYAN
1
From the Dept. of ENT Surgery, Baghiyatollah Hospital, Baghiyatollah University of Medical Sciences, Tehran. I.R. Iran.
The aims of surgical treatment for cholesteatoma include complete removal of
disease and formation of a dry, self cleaning ear in addition to hearing reconstruction.
This presentation concentrates on techniques of open mastoid surgery or modified
radical mastoidectomy to achieve a well-constructed cavity. This study analyzes
both primary and revision mastoid surgery referred to the author. All patients
presented with otorrhea, and four patients presented with facial paralysis, two with
labyrinthine and one with oval window fistula.
Sclerotic mastoid with extensive cholesteatoma were indications for modified
radical mastoidectomy as the treatment of choice. The main method of hearing
reconstruction in an open cavity with a mobile stapes was type III tympanoplasty,
leading to an improvement in the average air bone gap following surgery in both
primary and revision groups. The major causes of failure that were found when
revising mastoid cavities were incomplete removal of air cell tracts and inadequate
mastoid cavity formation without a sufficient meatoplasty. The techniques of open
mastoid or modified radical mastoidectomy that are required to avoid failure are
discussed.
http://mjiri.iums.ac.ir/article-1-934-en.pdf
Mastoidectomy
Cholesteatoma
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
175
177
article
ANTIOXIDANT VITAMINS IN DIABETES
A A. KHANAM
1
From the Department of Biochemistry, University of Karachi, Karachi-75270, Pakistan
The study included nineteen control subjects and fifty-nine diabetic patients.
The diabetic patients were divided according to the treatment they received. Fasting
blood glucose was markedly increased in all three groups, while low serum vitamin
A, C and E levels were observed in diabetic patients as compared to control subjects.
http://mjiri.iums.ac.ir/article-1-933-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
171
174
article
TEMPERATURE CORRECTION OF ARTERIAL BLOOD pH AND CO2 TENSION IN NEWBORN INFANTS: A NEW SOLUTION FOR AN OLD PROBLEM
A MADANI
1
F HABIB ZADEH
habibzaf@pearl.sums.ac.ir
2
M YADOLLAHIE
3
From the Division of Neonatology , Department of Pediatrics, Shiraz University of Medical Sciences
National Iranian Oil Company Outpatient Polyclinics, Shiraz, I. R. Iran
National Iranian Oil Company Outpatient Polyclinics, Shiraz, I. R. Iran
The acid-base status and arterial blood gas change with temperature variation.
To determine the changes in arterial pH and pC02, arterial blood samples of 50
neonates with different clinical conditions were examined at temperatures of 35
to 42°C with an increment of 0.5° C, which resulted in two nonlinear equations with
perfect fit on data. The temperature correction factors of these equations, unlike
factors of the previously proposed linear equations, are not, themselves, functions
of temperature. The arterial pH increases by 0.014 to 0.015 units, and peo2
decreases by 4.15-4.35%, with a 1ºC drop in body temperature.
http://mjiri.iums.ac.ir/article-1-932-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
167
170
article
E VALUATION OF ADENYLATE CYCLASE ACTIVITY IN MITRAL VALVE PROLAPSE
D QUJEQ
1
From the Biochemistry and Biophysics Department, Babol University of Medical Sciences, Babol, I.R. Iran
The term mitral valve prolapse (MVP) is used for a particular subset of patients
with hyperadrenergic dysautonomia. It occurs when part of a leaflet or both leaflets
of the mitral valve extend above the plane of the atrioventricular junction during
ventricular systole. The adenylate cyclase activity in MVP dys-autonomia was
studied by extraction of enzyme from the erythrocytes from 62 normal subjects and
78 MVP patients. Adenylate cyclase activity in the MVP group was increased
compared to that in controls [6.51 ±O.38 (mean±S.D., n= 78) vs. 2.76 ±O.12, n=
62, U activity/mg erythrocyte protein, p<O.05]. The determination of adenylate
cyclase activity in combination with echocardiography allows a reliable diagnosis
of MVP patients.
http://mjiri.iums.ac.ir/article-1-931-en.pdf
Mitral valve prolapse
MVP dysautonomia
adenylate cyclase.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
161
166
article
RESULTS OF CYTOGENETIC ANALYSIS OF 521 AMNIOTIC FLUID CELL CULTURES (AMNIOCENTESES) PERFORMED IN IRAN
A A. KARIMI-NEJAD
1
N LASHGARIAN
2
MH KARIMI-NEJAD
mhkarimi@mavara com
3
From the Karimi-Nejad Pathology and Genetic Center, Tehran, I.R. Iran.
The indications and results of cytogenetic analysis of 521 amniotic cell cultures
performed at 13-16 weeks of gestation were evaluated in this study. 507 fetuses
(97.3%) were cytogenetically normal, 14 (2.7%) had unbalanced karyotypes, and 2
fetuses were found to have major abnormalities, one with anencephaly detected by
measurement of alpha -fetoprotein levels in amniotic fluid and ultrasonography, and
the other with a full mutation at the FMRl locus detected by molecular techniques.
The unbalanced karyotypes included 2 cases each of trisomy 21, trisomy 18, triple
X, 47 XXY and mosaicisms and 4 cases of various chromosomal abnormalities.
225 tests were performed for women 35 years of age or over, 6 (2.4%) abnormal
karyotypes were detected, showing a 6 - fold increase over the general population.
This risk was even higher (2/23,8.7%) among those parous aged >35 y who had a
previous history of offspring with chromosomal aberrations.
5 of 32 (15.65%) fetuses whose parent was a carrier of a balanced chromosomal
translocation or a small chromosomal marker (in one case), had unbalanced
chromosomal aberrations.
There were 7 cases of spontaneous abortion within 4 weeks after amniocentesis,
one of which was a case of 46 XY, t (1421), + 18. Excluding this case, the rate of
abortion (6/521, 1.3%) was quite below the expected rate of 2.1 % for spontaneous
abortion in the 2nd trimester of pregnancy. Our data indicates that amniocentesis
performed at 13-16 weeks is a safe, reliable procedure for detection of fetal
chromosomal abnormalities in Iran, and we strongly recommend it for those parous
at risk.
http://mjiri.iums.ac.ir/article-1-930-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-11
13
3
0
0
article
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM
H TAJERZADEH
1
S SADRAY
2
From the Biopharmacy Division, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR.Iran, 14155/6451.
A simple, rapid and sensitive HPLC method for the determination of theophylline
(T) in human serum has been developed. An isocratic system consisting of a /-l
Bondapak C18 column, mobile phase of methanol, phosphate buffer (22:78, pH=4.5),
and a flow rate of 1.4 mL/min was used. The eluent was detected by UV at 275 nm
at room temperature. 8-Chlorotheophylline (8-CT) was used as an internal standard.
The serum samples deproteinated by methanol containing 8-CT and the supernatant
was injected into the HPLC system. The retention times of 5.7 and 8.1 min were
found for T and 8-CT, respectively. The linearity was checked in the range of 0.2-
30 µg/mL. Relative standard deviation for both inter-day and intra-day precision
analysis was less than 5%. No interference was observed from endogenous serum
components. Specificity was shown against some commonly co-administered drugs.
Simple and fast sample preparation, small sample volume (250 µL), precision,
reproducibility, specificity, sensitivity and high percentage recovery (98 %) make the
method to be practically useful for T monitoring in asthmatic patients.
http://mjiri.iums.ac.ir/article-1-936-en.pdf
Theophylline; Reversed-phasechromatography; 8-Chlorotheophylline; Therapeutic drug monitoring.