eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
81
81
article
CONGENITAL TRICHOFOLLICULOMA: A CASE REPORT
FARIBA IRAJI
1
FARAHNAZ FATEMI
2
From the Department of Dermatology, Shahid Beheshli Hospital, Isfahan University of Medical Sciences, Isfahan, IR.lran.
http://mjiri.iums.ac.ir/article-1-977-en.pdf
Trichofolliculoma
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
75
79
article
COMPARISON OF FOUR PCR TESTS FOR THE DETECTION OF MYCOPLASMA PNEUMONIAE
M HAJlA
1
Dp BACT
2
C.C. STOREY
3
From the Department of Microbiology, Faculty of Medicine, Medical Sciences University of Hamadan, Hamadan. I.R. Iran
From the Department of Microbiology, Faculty of Medicine, Medical Sciences University of Hamadan, Hamadan. I.R. Iran
the Micrabiology Department. University of Leeds, Leeds. U.K.
Recently PCR is being used more frequently as a diagnostic method to detect M. pneumoniae. We used primer pairs reported by Van Kuppeveld,13 Leng,5 Lunerberg,7 an Bernet' targeting 16s rRNA, PI protein, tuf genes. and a short DNA equence (MP5) to evaluate the sensitivity among different PCRs. Reoptimization experiments showed that tuf PCR had the highest sensitivity amongst these four PCRs, detecting 10 organisms. Detection limit for the rest of the PCRs was 100 copies of DNA. This study confirmed that 92° would be the best dissociation temperature rather than higher temperatures that are still being used frequently in other studies. Be ides. accurate optimizing of the annealing temperature and extension time had important roles on the sensitivity as well as using milli-Q distilled water rather than double distilled water. Experiments done on MP5 PCR proved that the non- specific products mentioned in previous studie 2 were not eliminated by increasing the annealing temperature, although they disappeared on gel electrophoresis after careful optimization of extension time.
http://mjiri.iums.ac.ir/article-1-976-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
67
73
article
EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE
ALI A FARHOUDI-MOGHADDAM
1
NICKOLAY N DOMANSKY
2
A.A ZIAEE
3
From the Molecular Biology Unit . Pasteur Institute. Tehran. I.R. Iran
The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 antigenic determinant. These vectors were transferred into the yeast. Only pDFl which contained the HBsAg gene was expressed. Some peculiar features of recombinant plasmid construction and expression of the HBsAg gene are discussed
http://mjiri.iums.ac.ir/article-1-975-en.pdf
HBV surface antigen
recombinant plasmids
Saccharomyces cerevisiae exoression vector.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
61
66
article
SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION
TAHEREH SHOKOHI
1
JULIE C. SILVER
2
From the Department of Parasitology, Mycology and Entomology. Mazandaran University of Medical Sciences, Sari, Islamic Republic of Iran
the Division of Life Sciences, Department of Microbiology and Molecular Genetics, Scarborough Campus. University of Toronto, Ontario. Canada
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus species isolated from bronchoalveolar lavage (BAL) fluid from patients with TA. In single PCR using the outer primers a specific 384-bp fragment was amplified. Similarly, y nested PCR with inner primers, a 357-bp fragment was amplified with DNA from Aspergillus fumigatus but not from the other microorganisms. The Southern blot hybridizations confirm the specificity of the PCR procedure for A. fumigatus using the cloned 374-bp PCR product probe. In conclusion, the nested PCR method appears to be quite rapid and specific.
http://mjiri.iums.ac.ir/article-1-974-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
55
60
article
ESCHERICHIA COLI HEAT-LABILE TOXIN B SUBUNIT: CONSTRUCTION AND EVALUATION OF PLASMIDS PROVIDING CONTROLLED HIGH LEVEL PRODUCTION OF THE PROTEIN
MR AKBARI EIDGAHI
1
MH SHAH-HOSSEINY
2
B TABARRAI
3
V RECHINSKY
4
From the Biochemistry Dept . Pasteur Institute of Iran. Tehran
the Biology Dept .. Faculty of Science. Imam Hosein University. Tehran.
From the Biochemistry Dept . Pasteur Institute of Iran. Tehran
Office for Scientific and Industrial Studies. Presidency. Tehran. I.R. Iran.
With the plasmid DNA from a clinical isolate of enterotoxigenic Escherichia coli (ETEC) H 10407 as template, PCR-mediated cloning of the sequence encoding the heat-labile toxin B subunit (L T -B) has been carried out. Then this sequence was recloned into the pTrc 99A and pET23a expression vectors to give the pJasmids pTRCLTB and pETLTB, respectively. After induction, the former plasmid provides for the production of rL T-B in a yield of up to 15 mg per liter of bacterial culture. The recombinant protein was shown to be structurally and immunologically identical with the native L TB. High titer antibodies capable of neutralizing the native toxin were raised in mice by oral administration of the rL T-B. Hence the constructed plasmids provide the basis for an oral ETEC vaccine, as well as for genetic fusion of foreign antigens with the aim of developing polyvalent vaccines.
http://mjiri.iums.ac.ir/article-1-973-en.pdf
ETEC. Recombinant L T -B. Oral vaccine. PCR.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
51
54
article
THE EFFECT OF CARRAGEENAN-INDUCED INFLAMMATION ON BLOOD FLOW IN THE RABBIT KNEE JOINT
A A. KHOSHBATEN
1
WR FERRELL
2
WJ ANGERSON
3
H NAJAFIPOUR
4
From the Dept. of Physiology and Biophysics, Baghiyatollah (a .s.) University ofMedical Sciences, Tehran ,
the Institute of Physiology, University of Glasgow, 8QQ U.K
the Institute of Physiology, University of Glasgow, 8QQ U.K
the Dept. of Physiology, Kerman University of Medical Sciences, Kerman , l. R.lran.
Experiments were carried out on anesthetized adult New Zealand rabbits. Acute joint inflammation was achieved by intra-articular injection of 2% carrageenan. Radiolabelled microspheres were used to measure the effect of acute inflammation on blood flow in the knee joint and establish whether neural influences on blood vessel caliber were affected. Surgical exposure of the posterior joint "capsule" increased blood flow in the control knee, but subsequent section of the posterior articular nerve (PAN) supplying the knee produced no further increase. In the inflamed knee, blood flow declined progressively with ea<.:h procedure. Electrical stimulation of PAN reduced blood flow in the posterior region of both knees, with somewhat less effects in the inflamed knee.
http://mjiri.iums.ac.ir/article-1-972-en.pdf
Knee joint. Blood flow
Microspheres. Carrageenan.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
43
50
article
LOW INTENSITY PULSED ULTRASOUND TREATMENT INCREASES RABBIT RADIAL FRACTURE HEALING
GD NAYERI KAMAN
1
S KESHTGAR
2
From the Department of Physiology. School of Medicine. Shiraz University of Medical Sciences. Shiraz. I.R. Iran.
In order to study the effect of ultrasound (US) on bone healing, a complete
transverse fracture was made in the right radius of adult male rabbits by a cutter.
52 rabbits were used: group I. control (n= 17): group 2. test (n:=: 15): group 3, intact
(n= I) and group 4 (n= 12) who were examined only for evaluation of the cutter
effects in detail. 7 animals were operated with a Gigli saw. The test group received
US treatment at 0.5 W /cm2• I MHz, 2 msec on-8 msec off for 10 min/day, from the
day after surgery until complete fusion was observed. Radiological studies
indicated that mean healing duration and rate of healing was significantly more in
the test group than in controls. Histological evaluation showed the presence of
ossified callus at week 3 in the test group. but in controls fibrous callus was still
seen at week 5 after surgery. Bone mineral analyses by stereoelectron microscope
showed that the mineral component of the treated bone revert to normal, similar
to the composition of intact bone. and sooner than that of the control. However, our
results revealed no deleterious effects of US on treated and untreated ipsilateral and
contralateral bones during the experiment or one month after complete fusion of
the bones, at which time US was terminated
http://mjiri.iums.ac.ir/article-1-971-en.pdf
Ultrasound
Healing. Fracture. Bone.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
37
42
article
MOLECULAR CHARACTERIZATION AND OPTIMIZATION OF VI-CAPSULAR POLYSACCHARIDE OF SALMONELLA TYPHI TY6S PRODUCTION IN BIOREACTOR
HOJJAT AHMADI
1
BAHMAN TABARAlE
2
MEHDI NEJATI
3
SAEID AL-e AGHA
4
From the Department of Bacterial Vaccine. Research and Production Complex, Pasteur Institute of Iran,
From the Department of Bacterial Vaccine. Research and Production Complex, Pasteur Institute of Iran,
From the Department of Bacterial Vaccine. Research and Production Complex, Pasteur Institute of Iran,
Razi Vaccine and Serum Research Institute. Hesarak, I.R. Iran.
The role of Vi-capsular polysaccharide (Vi-CPS) in human immunity against infection caused by Salmonella typhi is well known. The downstream process of purification generally causes depolymerization of Vi-CPS to a nonimmunogenic low molecular weight form. In the present study, a standard strain of Sal. typhi Ty6s was grown under submerge cultural conditions in a pilot-plant scale of 90 Liter fermentor. At the late exponential growth phase, crude polysaccharide wa obtained from fermentation broth by detergent-phenol extraction method and purified by ultracentrifuge differentiation technique. Analytical data reveals that by optimization of fermentation parameters, not only was the yield of production increased from 1.6 mg/L to 4.9 mg/L, but also the polysaccharide retained its native molecular stability and immunogenicity. Therefore, purified Vi-CPS can be regarded as a reliable immunogen to control typhoid fever in man.
http://mjiri.iums.ac.ir/article-1-970-en.pdf
Salmonella typhi. Vi-CPS
Polysaccharide vaccine.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
33
36
article
CLINICAL MANIFESTATIONS AND DIAGNOSIS OF CELIAC DISEASE IN CHILDREN
MR ESMAILI DUKI
1
GR KHATAMI
2
M NAJAFI
3
A KHODADAD
4
From the Department of Pediatrics, Gastroenterology Division, Children's Medical Center, TehrOl1 University of Medical Sciences, Tehran, Islamic Republic of Iran.
Celiac disease (CD) is defined as a permanent intolerance to gluten with clinical manifestations and abnormal small intestinal mucosa. A case series study was performed to obtain information about clinical manifestations, age of presentation and diagnosis of CD. For this purpose, hospital records of patients who were admitted to the gastrointestinal ward in the Children's Medical Center from 1986 to 1996 with suspected diagnosis of CD and discharged with such a diagnosis were reviewed. Thirty-five patients (17 male, 18 female) with diagnostic criteria for CD were selected and followed, The average age of onset was 3.07 years but mean age of diagnosis (the first small intestinal biopsy) was 7.7 years. The most common symptom was chronic diarrhea. The interval between initial symptoms and diagnosis was surprisingly long. During this period these subjects had multiple medical visits at different hospitals. With recognition of this treatable disorder and by available diagnostic procedures and obtaining small intestinal biopsy specimens, we can reduce the mentioned interval and diagnose this entity earlier in Iranian children.
http://mjiri.iums.ac.ir/article-1-969-en.pdf
Celiac disease. Gluten
Children
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
27
32
article
THE SYNTHESIS AND EVALUATION OF NEW DERIVATIVES OF 2,4 DIAMINOPYRIMIDINES WITH MALE CONTRACEPTIVE ACTIVITY
H SADEGHIPOUR ROODSARIE
1
M VOSOOGHl
2
M ZAHRAIE
3
From the Departments of Physiology Tehran University of Medical Sciences. Tehran. I.R. lran.
From the Departments of 'Medicinal Chemistry Tehran University of Medical Sciences. Tehran. I.R. lran.
From the Departments of Clinical Biochemistry. Tehran University of Medical Sciences. Tehran. I.R. lran.
The discovery of the antifertility activity of gossypol led scientists and researchers to the development of compounds with antifertility activity that can be used as male contraceptives. These studies resulted in discovery of the antifertility activity of several classes of compounds which have been reported in the literature. This article deals with the synthesis of four new analogues of pyrimethamine, the derivatives of 2, 4-diamino-5 (3,4-dichlorophenyl)-6-alkyloxymethyl pyrimidine (methyl ethyl, n-propyl, iso-propyl), and the results of experimental antifertility evaluation of these compounds on male rats, which revealed a significant decrease in motility, viability, ESR and DSP indices in treated animals
http://mjiri.iums.ac.ir/article-1-968-en.pdf
2
4-Diaminopyrimidines; Male contraception; Male reproduction; Spermatogenesis; Fertility
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
19
26
article
CHILDREN\'S RISKY ACTIVITIES AND PARENTS\' IDEAS ON CHILDREN\'S RISK-TAKING BEHAVIOUR
HAMlD SOORI
1
From the Department of Community Medicine, Medical School, Ahwaz University 0f Medical Sciences, P. O. Box. 61355•45, Ahwaz, I. R. lran.
A cross-sectional study with children's and parents' self-completed questionnaires was carried out to evaluate parents' ideas on children's risk-takjng behaviours and children's risky activities after school hours by age (7 and 9 years) and sex. Nine elementary schools were randomly selected and 476 pupHs aged seven and nine years and 471 parents were studied. Most parents (90.1 %) believed that their children take risks when they play outside after school anyway. The top three risky activities were climbing walls (25.0%), climbing trees (14.8%) and riding carelessly (14.1 %). Overall, boys were more likely to take risks than girls (47.4% vs. 33.6%, p<O.O I). Although more needs to be learned about children's after-school risk-taking behaviours, it is clear that the pattern of risk-taking behaviour for younger children is different from that of older ones. Boys were more likely to take risks and are at greater risk of being involved in an accidental injury when they play outside after school than girls. Because many children do take risks after school, a broad and balanced approach combining educational and environmental componen ts is essential to prevent after-school childhood accidents.
http://mjiri.iums.ac.ir/article-1-967-en.pdf
Injury
Children. Risk
Behaviour
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
15
17
article
SURGICAL TREATMENT OF ULCERATIVE COLITIS
SOHRAB ATEFI
1
From the Department of Surgery, Shiraz University of Medical Sciences, Shiraz, I.R. Iran.
This is a preliminary report of 28 patients with ulcerative colitis who we surgically treated with "J" pouch endorectal pull-through operation from 1978 to 1996. All patients are continent with a median stool frequency of 4 times per day, no night soilage. low postoperative complications and one mortality due to pulmonary embolism.
http://mjiri.iums.ac.ir/article-1-966-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
11
14
article
CORRELATION BETWEEN THE SEVERITY OF CARDITIS AND THE LEVEL OF ACUTE PHASE REACTANTS AND ANTI-STREPTOLYSIN 0 TITER IN ACUTE RHEUMATIC FEVER: A RETROSPECTIVE STUDY IN SHIRAZ
MR SABRI
1
MR KADIVAR
2
From the Cardiology and Units, Department of Pediatrics. Nemazee Hospital. Shiraz . I.R. lran.
From the lnfectious Disease Units, Department of Pediatrics. Nemazee Hospital. Shiraz . I.R. lran
In order to find the correlation between the severity of carditis in acute rheumatic fever (ARF) and the erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and anti-streptolysin O (ASO) titers, we performed a retrospective study in Shiraz on one-hundred and four patients. The mean age of the patients was 11 years. Fever was seen in 85.5%, arthralgia in 95.2%, arthritis in 81.7%, carditis in 82.5%, chorea in 3.8%, and subcutaneous nodules in 0.9% of patients. Mitral regurgitation was the most frequent valvar involvement (79.6%). Sixteen patients (15.4%) had severe carditis. The ESR level was ≥20 Wintrobe units in 98.0%, the CRP was elevated in 83.0%, and the ASO titer was ~400 Todd units in 91.0% of patients. There was no significant statistical difference between those patients with mild and severe carditis concerning the level of ESR, CRP, and ASO titers. There was also no significant statistical difference between the level of these parameters and the presence or absence of carditis, and the patient's age (≤8 years or >8 years old).
http://mjiri.iums.ac.ir/article-1-965-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
5
10
article
A ROTATORY MANEUVER TO REDUCE IMBALANCE OF THE VESTIBULAR SYSTEM IN PATIENTS WITH VESTIBULAR VERTIGO
N RASSAIAN
1
S SADEGHI GHANDEHARI
2
B SABETAZAD
3
From the Department of Physiology-Biology, Shaheed Beheshti University of Medical Sciences, Tehran,
In an attempt to diminish the intensity of vertigo, this study was designed to
reduce the imbalance between the vestibular system on the two sides. By rotating the
subjects with a string-activated rotating chair in opposite direction of the dominant
side, the dominant side would be inhibited and simultaneously the subordinate ide
would be stimulated. Eight patients with vestibular vertigo confirmed by ENG and
rotatory test were subjected to a rotatory stimulus, the effect of which was evaluated
by rotatory test. V mal of the two sides of all patients were compared before the
stimulus and to, 40, and 70 minutes afterwards. The result indicate a significant
difference between directional preponderance COP) values before and after. stimulation,
especially at 70 minutes. The initial values of DP in 5 of 8. patients were out of the
normal range and were significantly reduced to lower levels after timulation,
especially after 70 minutes when DP fell within normal limits. In two of the
remaining patient , a change in direction was observed at 40 minutes, and it remained
'0 even after 7 minute in one of them. By minimizing the inequality between the
vestibular discharge on the two sides, a reduction in vertigo intensity is expected
http://mjiri.iums.ac.ir/article-1-964-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-05
13
1
1
4
article
EVALUATION OF SERUM IM MUNOGLOBULINS AND THEIR RELEVANCE TO CHRONIC LUNG DISEASE IN MUSTARD GAS VICTINIS
H SOHRABPOUR
1
MR EBRAHIMI-RAD
m.ebrahimi@bmsu. ac.ir
2
From the Dept. of Internal Medicine, Pulmonary Division, Labbafinejad Hospital, Shahid Beheshti University of Medical Sciences, Tehran
the Legal Medicine Organization of Iran. Tehran, I .R. Iran.
Despite the fact that 8 years has elapsed since the end of the imposed war,
chemically injured victims are still suffering from various long-term complications,
most of them respiratory in nature. The progressive nature of respiratory problems
in these patients suggests that the immune system must be involved to Initiate a
cascade which ends up causing lung injuries.
To evaluate this, humoral immunity was assessed in 179 mustard gas victims
in 1992. The patients were followed up for the next 4 years. These included 172
male and 7 female patients with a mean age of 33 years and an average of 6.1 years
post-exposure to mustard gas. After physical examination and spirometriC
evaluation, these patients were divided into 3 groups based on the severity of their
respiratory problems, i. e. "severe" (group 1 ), "moderate" (group 2) and "mild"
(group 3). IgG, IgA, C3 and C4 were measured using SRlD technique and IgE by
ELISA. The results in each group were compared with the control group consisting
of 49 healthy, randomly selected volunteers. Mean age was 32 years in this group.
The results indicate that there is a significant fall in IgG, IgA, C3 and C41evels
in group 3 (p=0 .009, 0 .01 , 0 .004, and 0.00 2, respectively) as compared to the
control group. IgG had also dropped significantly in groups 2 and 1. On the other
hand in group 3, 19.6% and 15.2% of patients had lower than normal IgG and IgA
levels, respectively. In group 2, 7.7% of patients had low IgG and 5.8% had low
IgA levels. These figures were 10 % and 3.8% for IgG and IgA in group 1. Of a total
of7 patients in group 3 with low IgA levels, four patients also had low IgG levels.
Two of these patients gradually developed more serious rspiratory problems
and were classified as group 1 after two years.
We conclnded that: 1) mildly injured patients (group 3) have lower IgG and IgA
as well as C3 and C4 levels, and 2)among these patients those who demonstrate low
IgG levels are more prone to develop progressive respiratory problems in the
future.
http://mjiri.iums.ac.ir/article-1-963-en.pdf
Mustard gas
Immunoglobulins
ELISA.