eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
393
397
article
THE ROLE OF VARIOUS STABILIZERS IN THE ACQUISITION OF THERMO-TOLER ANCE IN FACTOR VIII ACTIVITY
T ZANDIEH
1
M FARHADI
2
M SHAHR-ABADI
3
H LOTFI
4
From the Iranian Blood Fractionation and Research Company, Tehran
the Iranian Blood Transfusion Service, Tehran
the Virology Lab, Rasool Akram Hospital, Tehran, I.R. Iran.
From the Iranian Blood Fractionation and Research Company, Tehran
We prepared a highly purified and relatively heat stable form of factor VIII
which contained 25 units per mL (u/mL) activity using PEG-4000 and developed an
effective and new manufacturing process. Heat treatment was performed at 80°C for
72 hrs in the presence of different stabilizers. In our studies, we used different organic
solvents as preservatives to maintain factor VIII activity, since few enzymes have
been observed to function perfectly well in such conditions. Our results show that the
activity of factor VIII in the presence of organic solvent at 80°C for 72 hrs as viral
inactivation is significantly decreased. It seems that irreversible aggregation of
protein is the major mechanism in cessation of factor VIII activity. On the other hand,
protein stability may be increased by certain low molecular weight substances. We
therefore used different sugars and amino-acids to protect factor VIII activity in the
process of heat treatment. Our results showed that glycine, glucose, saccharose,
mannitol and BSA were not effective stabilizers of factor VIII. But the loss of factor
VIII activity at 80°C for 72 hrs, in the presence of 10, 15 and 20% concentrations of
trehalose, was about 15-20%, which is acceptable for large-scale processes. Thus, the
condition obtained in the present study seems to be a more suitable condition than
that reported previously.
http://mjiri.iums.ac.ir/article-1-994-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
387
391
article
SIMULTANEOUS DETECTION OF CHLAMYDIA PNEUlvlONIAE AND MYCOPLASMA PNEUMONIAE BY PCR
M HAJIA
1
C.C STOREY
2
From the Microbiology Department, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, I.R. Iran
and the Microbiology Department, University of Leeds, Leeds, UK.
Both C. pneumoniae and M. pneumoniae are common causes of respiratory tract
infection. At present, both are still diagnosed in the laboratory retrospectively by
serology. This is despite many publications which indicate that PCR, which is not
retrospective, is extremely good at detecting these organisms. We thought that a
single PCR test which could detect both organisms simultaneously in a routine
diagnostic laboratory would be more economic than using two separate PCR tests.
Chlamydia PCR was developed and optimized to detect C. pneumoniae using
primer CpnA and CpnB which targets the MOMP gene. This test was very sensitive
and could detect 10 organisms. To detect M. pneumoniae, the tu!PCR reported by
Luneberg6 was selected. After optimization of the duplex test, it was found that the
sensitivity of the test for mycoplasma PCR was 100 times less than the sensitivity of
the single tests, due to the inhibitory role of C. pneumoniae primer CpnB. Reducing
the concentration of this primer helped but we decided to redesign it instead. The final
form of the duplex has sufficient sensitivity, detecting 10 copies of each organism.
The new primer CpnB2 was a great improvement. The test was then developed to
detect the product by hybridization rather than analysis with agarose gel
electrophoresis.
http://mjiri.iums.ac.ir/article-1-993-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
381
386
article
ANTINOCICEPTIVE INTERACTION BETWEEN ADENOSINE AND CARBAMAZEPINE IN MICE
H HOSSEINZADEH
1
ME SHOOSHTARI
2
From the Department of Pharmacodynamy and Toxicology, Faculty o/Pharmacy, Mashhad University of Medical Sciences, P.O. Box 91775-1365, Mashhad, I.R. Iran.
There are several reports that adenosine and carbamazepine have
pharmacodynamic interaction. I n this study the antinociceptive interaction o f these
two agents was evaluated in mice by the hot plate test. Agents were injected
intraperitoneally.
0.2 and 0.4 mg/kg doses of R-phenylisopropyladenosine (R-PIA) substantially
showed antinociceptive effects. Carbamazepine had antinociceptive actions except
at a dose of 0.8 mg/kg. Theophylline (12.5 mg/kg), as a non-selective antagonist of
adenosine receptors, showed a nociceptive effect. Dipyridamole, an uptake blocker
of adenosine, had no antinociceptive effect even with a dosage of 90 mg!k:g.
Theophylline did not decrease the antinociceptive effect of carbamazepine, while
dipyridamole increased the antinociceptive effect of carbamazepine 30 min utes after
administration. Carbamazepine (8 mg/kg) inhibited the antinociceptive effect of RPIA.
This study supports the possibility that the interaction between adenosine and
carbamazepine may be related to their actions at adenosine receptor sites in the brain.
http://mjiri.iums.ac.ir/article-1-992-en.pdf
Adenosine
R-PIA
Carbamazepine
Theophylline
Dipyridamole
Antinociceptive.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
371
376
article
EFFECTS OF HYPOXIC HYPOXIA AND CARBON MONOXIDE-INDUCED HYPOXIA ON THE CARDIOVASCULAR SYSTEM AND REGIONAL BLOOD FLOW OF THE ANESTHETIZED CAT
GHOLAM A. DEHGHANI
1
From the Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran.
The purpose of this study was to investigate the potential responses of the
cardiovascular system and regional blood flow to hypoxic hypoxia (BB) and to
carbon monoxide (CO)-induced hypoxia (COH). Ten anesthetized cats were studied
under two nonnoxic (control: CONT) and two hypoxic conditions. Four types of
radioactive micro spheres were used to measure regional blood flow during CONT
and two hypoxic conditions. During CONT the animal was ventilated with 22% 02'
5% CO2 and N2 (room air). HH was induced by ventilating the cat for 15 min with
6-8% O2, and COH by adding 0.1 % CO to room air and reducing blood oxygen
content to the same level as HH. Cardiac output and contractility significantly
increased (pO.05).
Gastric blood flow (14±2 CONT) only increased during HH (22±4) but splenic
blood flow (119±2 CONT) decreased with both HH (40±9) and COH (37±9).
Regional blood flow of other segments measured showed a mixed response to HH
and COH. In conclusion, it seems that: 1) systemic hypoxia would stimulate the heart
to increase its output to maintain Pa and overcome the increased demand of some
organs, and 2) the different responses of regional vascular beds to HH and COH may
be due to various sensitivities of each organ to arterial blood oxygen tension and
autonomic neuro-hormonal controls that have originated from stimulations of aortic
and carotid chemoreceptors.
http://mjiri.iums.ac.ir/article-1-990-en.pdf
Systemic hypoxia; arterial chemoreceptors; cardiovascular system; regional blood flow.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
365
369
article
INFLUENCE OF CCK RECEPTOR AGONIST AND ANTAGONISTS ON TOLERANCE INDUCED TO ANTIPENTYLENETETRAZOL ACTIVITY OF DIAZEPAM
M REZAYAT
1
H HOSSEINI
2
J AKHONDIAN
3
N VAHDATI
4
MS ZARRINDAST
5
From the Department of Pharmacology, Tehran and Mashhad Universities of Medical Sciences, I.R. Iran.
In the present study, the effects of caerulein and CCK receptor antagonist< on
tolerance to the anti-pentylenetetrazol activity of diazepam has been studied.
Different doses of diazepam (20, 30, 35, 40 and 50 mg/kg) were administered
intraperitoneally for a period of 6 days in order to induce tolerance to diazepam. 48
h after the last dose of diazepam, a test dose of diazepam was tested for antipentylenetetrazol
activity. When animals were treated with caerulein or the CCK
receptor antagonists MK -329 and L-365,260, only L-365,260 reversed the tolerance
to diazepam.
It may be concluded that CCK-B receptor mechanisms may interact with
tolerance induced by diazepam.
http://mjiri.iums.ac.ir/article-1-989-en.pdf
CCK agents
diazepam
pentylenetetrazol anticonvulsion test
mice.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
359
364
article
INVOLVEMENT OF THE SEROTONIN SYSTEM IN SSRI-INDUCED ANTINOCICEPTION
M RAFIEIAN-KOPAEI
1
R.D.E SEWELL
2
From the Department of Pharmacology, Shahrekord University of Medical Scier.ces, Shahrekord, I.R. Iran
the Welsh School of Pharmacy, UWC, Redwood Bldg, King Edward VII Ave., Cardiff, CFI 3XF, U.K.
Serotonin specific reuptake inhibitors (SSRI) may induce antinociception
however, the mechanism of this effect is not clear. SSRls increase 5-HT levels in
neuronal synapses and facilitate serotonergic activity. In this study, therefore, the
activity of para-chlorophenylalanine (pCPA), which reduces 5-HT release, and 5-
hydroxy tryptophan (5-HTP), a precursor of 5-HT, were examined on the
antinociceptive activity of six SSRls, in the abdominal constriction test. The
compounds studied included fluoxetine, fluvoxamine, sertraline, paroxetine,
citalopram and zimelidine. The effects of pCPA and 5-HTP were also evaluated on
morphine analgesia as a standard compound. All antidepressants tested demonstrated
dose-inhibition of acetic acid-induced abdominal constrictions. The antinociceptive
activities of morphine, fluoxetine, fluvoxamine and sertraline, but not paroxetine,
citalopram and zimelidine were significantly reduced by pCPA. Subsequently, 5-
HTP restored the reduced antinociception of morphine, fluoxetine and fluvoxamine
caused by pCP A. Furthermore, 5-HTP increased morphine, fluoxetine, fluvoxamine
and sertraline-induced antinociception. Opioid receptor antagonists have been
shown to reduce the antinociception induced by morphine, fluoxetine, fluvoxamine
and sertraline but not by paroxetine, citalopram and zimelidine. It can be concluded,
therefore, that the serotonin system is only involved in antinociception produced by
antidepressants, and their antinociception is opioid antagonist reversible.
http://mjiri.iums.ac.ir/article-1-988-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
353
357
article
PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI
MANA OLOOMI
1
SAEID BOUZARI
2
VLADIMIR O RECHINSKY
3
From the Molecular Biology Unit, Pasteur Institute of Iran, Tehran I.R. Iran.
The human granulocyte-macrophage colony stimulation factor (hGM-CSF)
gene was cloned in the pET 23a( +) expression vector under the control of strong
bacteriophage T7 transcription and translation signals. The hGM-CSF gene was
transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction
of GM-CSF gene. Production of the target protein was obtained as revealed by
ELISA and Western blot analysis. The produced 'h:GM-CSF was purified by
immunoaffinity chromatography. The dot blot positive fractions were assayed for
biological activity and it was shown that the expressed GM-CSF is active.
http://mjiri.iums.ac.ir/article-1-987-en.pdf
Human GM-CSF
Gene Expression
Purification
E. coli.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
345
351
article
BRONCHODILATORY AND ANTICHOLINERGIC EFFECTS OF CARUM CARVI ON ISOLATED GUINEA PIG TRACHEAL CHAINS
MH BOSKABADY
1
M TALEBI
2
From the Dept. of Physiology, Ghaem Medical Center, Mashhad University of Medical Sciences, Mashhad, I R. Iran.
Carum carvi (CC) is a grassy plant which is believed to have several therapeutic
effects, including anti-asthmatic properties. We have therefore studied the
bronchodilatory and anticholinergic effects of this plant.
The bronchodilatory effects of the aqueous extract (AE), macerated extract
(ME), essential oil (EO), and 4µM theophylline (T) in comparison with saline (S)
were examined by their relaxant effects on precontracted [by 10 µM
methacholine (M)] isolated tracheal chains of guinea pigs (n=1O). The
anticholinergic effects of AE, ME, EO, and 5 nM atropine (A) were tested by
comparing the cumulative Log concentration-response curves (LCRCs) of Minduced
contraction of tracheal chains and the effective concentration of M
causing 50% of maximum response (EC50) in the presence of AE, ME, EO, and A
with that of S.
Although the bronchodilatory effects of AE, ME, and EO were lower than that
Of T (p<0.001 for all cases), they were significantly higher than the effect of S (p<
0.05 for AE, p<0.01 for ME, and p<0.005 for EO). There were also clear nonparallel
right-ward shifts in LCRCs obtained in the presence of AE, ME, and EO,
but a parallel shift in the presence of A compared to the curve obtained in the
presence of S. The slope of LCRCs obtained in the presence of AE, ME, and EO
were significantly lower than that of S (p<O.OI for AE, p<0.05 for ME, and
p<0.001 for EO). The ECso obtained in the presence of AE (p<0.005), ME
(p=O.005), EO (p<0.02), and A (p<0.001) were significantly higher than that of S.
However, the EC50 values obtained in the presence of AE, ME, EO and dose ratio
(DR-I) produced by ME were not significantly different from that of A. The
LCRCs obtained in the presence of EO on incubated tracheal chains with 1µM
propranolol and 1 µM chlorpheniramine also showed a clear non-parallel rightward
shift comparing with that of S. However, the slope of this curve was
significantly higher than that of non-incubated experiments (p<0.05).
The results of this study indicated that the bronchodilatory effect of Carum
carvi is mainly due to the non-competitive antagonistic property of this plant at
muscarinic receptors. The β stimulatory effect and/or anti-histaminic effect of EO
might be contributed to its non-competitive property. The variation between
anticholinergic behaviours of different extracts is probably due to the variation of
methods used, leading to extraction of different substances or destruction of some
substances due to high temperature.
http://mjiri.iums.ac.ir/article-1-986-en.pdf
Carum carvi
bronchodilatory
anticholinergic
guinea pig.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
339
343
article
EFFEC T OF AZELAIC ACID ON SEBUM EXCRETION RATE: A C OMPARATIVE STUDY BETWEEN TWO SAMPLING TECHNIQUES
ES FARBOUD
1
M ADRANGUI
2
the Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran
the Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, I.R. Iran.
In this study the influence of azelaic acid (AZA) 20% cream on the sebum
excretion rate (SER) of 80 volunteers having mild to moderate acne vulgaris was
demonstrated. Absorbent paper and Sebutape were used to collect the sebum and
quantification was carried out by direct gravimetric method. It was shown that topical
application of AZA creams could reduce the SER and number of acneic lesions as
well as the grade of acne. Sebutape has the advantage of simplicity and reproducibility
and direct weighing of Sebutape is preferred to the classic scoring method. A near
correlation was observed between the two sampling methods, i.e., absorbent paper
and Sebutape.
http://mjiri.iums.ac.ir/article-1-985-en.pdf
Azelaic acid
Sebum excretion rate
Acne
Sebutape
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
337
380
article
LACK OF ANTICONVULSANT ACTIVITY OF INTRACEREBROVENTRICULAR PROGESTERONE AND ALLOPREGNANOLONE IN MALE AMYGDALA-KINDLED RATS
M SAYYAH
1
MH POURGHOLAMI
2
From the Department of Pharmacology, Faculty of Medicine, Shaheed Beheshti University of Medical
The effect of intracerebroventricular administration of progesterone and its
metabolite allopregnanolone(3a-hydroxy-5a-pregnane-20-one)on the amygdala
kindling model of seizures was studied in male rats. Neither progesterone (10, 50,
200 g/rat) nor allopregnanolone (12.5, 50, 100 µg/rat) had any effect on seizure
parameters at 5, 15 and 30 min. after administration. Although previous studies
imply the anticonvulsanteffect of progesterone and its metabolite allopregnanolone
against seizures induced by GABAA receptor antagonists, the present data do not
support the anticonvulsant activity of these two steroids in amygdala-kindled
seizures.
http://mjiri.iums.ac.ir/article-1-991-en.pdf
Seizure
Progesterone
Allopregnanolone
Amygdala-kindled rats.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
333
338
article
DIFFEREN TIATION BETWEEN PROSTATIC\' C A RCINOMA A ND BENIGN PROSTATIC HYPERPLASIA BY AgNOR STAINING
SIMIN TORABI-NEZHAD
1
PARVIN KHERADMAND
2
From the Department of Pathology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, IR. Iran.
Thirty cases of benign prostatic hyperplasia and 30 cases of prostatic
adenocarcinoma were selected and stained by AgNOR (argyrophilic staining of
the nucleolar organizer region) method.
Specimens were obtained by either prostatectomy (open!TIJR) or needle
biopsy of the prostate.
In this study, the sections of prostatic adenocarcinoma were reviewed. 6 cases
were diagnosed as well-differentiated, 12 cases as moderately-differentiated and
12 cases as poorly differentiated adenocarcinoma.
The method of AgNOR staining was applied to see if it would be useful in the
distinction between benign and malignant lesions. In benign lesions, the mean
number of AgNOR count was 2.5 and in malignant specimens was 7.4. Prostatic
hyperplasia (benign) specimens showed two or three well-defined NORs, but in
malignant tumors, there were many irregular small NORs. AgNOR stain can be
used for differentiation between benign and malignant lesions of the prostate, but
this stain should not be used as a reliable index for grading prostatic adenocarcinoma,
because overlapping was seen in some cases.
http://mjiri.iums.ac.ir/article-1-984-en.pdf
AgNOR staining
Prostatic carcinoma
Benign prostatic hyperplasia
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
329
331
article
DISCRIMINATION OF HEPATOCELLULAR CARCINOMA FROM CIRRHOTIC NODULES BY AgNOR STAIN
ALI ZARE MEHRJARDI
1
MEHDI MASSOUMIAN
2
From the Department of Pathology, Kerman University of Medical Sciences, Kerman, I.R. Iran
Discrimination of regenerative cirrhotic nodules of the liver (CN) from
hepatocellular carcinoma (HCC) is sometimes difficult. We examined the utility of
AgNOR staining in this context. Fifteen cases of HCC and 25 cases of CN were
stained by AgNOR method and the mean AgNOR number for one hundred nuclei in
each case was determined. There was a significant difference between the mean
AgNOR counts in CN (6.63±4.00) and HCC (11.36±2.88) (p<0.0005).
Discriminant analysis showed that all HCC cases and over 90% of CN could be
correctly distinguished by this method. It seems that AgNOR staining is an accurate
and readily available tool for differentiating CN from HCC.
http://mjiri.iums.ac.ir/article-1-983-en.pdf
Cirrhosis
Cirrhotic nodule
Hepatocellular carcinoma
AgNOR.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
325
328
article
DI AGNOSTIC VAL UE OF SUBL INGUAL NITROGLYCERIN TILT TEST: COMPARISON WITH THE STANDARD ISOPROTERENOL TILT TEST
SAEED ORAII
Oraii@ rhc. ac. ir.
1
MAJID MALEKI
2
MEHRNOOSH MINOOIl
3
PARIVASH KAFAII
4
MAHMOOD EFTEKHARZADEH
5
From the Cardiology Department of Shahid Rajaii Heart Hospital. Iran University of Medical Sciences. Tehran. I.R. Iran
Sublingual nitroglycerin (TNG) has been introduced as a promising provocative
agent for tilt table testing, but it has not been compared directly with the standard
isoproterenol (ISO) infusion test previously. We tried to assess the diagnostic value
and safety of TNG tilt testing as compared with ISO infusion in patients with
unexplained syncope.
TNG and ISO tilt tests were performed in two successive days on a random basis
for both cases and controls. 65 consecutive patients with unexplained syncope after
thorough work-up and 20 healthy volunteers were recruited into the study. Positive
responses were observed in 20 patients (31 %) during the passive phase, 25 (55% of
cases or 38% of total) during the TNG phase and 26 (58% or40% of total) during the
ISO phase. In the control group, positive responses during the passive, TNG and ISO
phases occurred in 1,1 and 2 cases, respectively. The sensitivity and specificity of the
tests can be summarized as 69% and 90% respectively for the TNG test versus 71 %
and 85% respectively for the ISO test. Owing to discordant responses in 75% of the
cases, the sequential use of the tests (if one is negative) would increase the sensitivity
to 89% while decreasing the specificity slightly (to 80%). Side effects were also less
frequent with TNG. We conclude that sublingual TNG testing is an effective and safe
alternative to the ISO infusion test and can be used as a complementary test.
http://mjiri.iums.ac.ir/article-1-982-en.pdf
Syncope
Tilt table test
Isoproterenol
Nitroglycerin.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
319
323
article
ANTI-TETANUS IMMUNOGLOBULIN ISOTYPES IN WOMEN OF CHILDBEARING AGE
K AHMADI
1
MB ESLAMI
2
From the Department of Immunology, Baghiatollah University of Medical Sciences, Tehran
the School of Public Health, Tehran University of Medical Sciences, Tehran, I.R. Iran.
Neonatal tetanus (NT) is a leading cause of neonatal mortality in many parts of
the world. Tetanus is a vaccine-preventable disease and is second only to measles
worldwide as a cause of childhood mortality. In this study the various irnrnunoglobulin
classes of anti-tetanus antibody in the sera of 105 Iranian women of childbearing age
(13-45 years) were titrated by enzyme linked immunosorbent assay (ELISA). Our
results demonstrate that the majority of women (96.29%) had protective anti-tetanus
toxoid antibody. All women were negative for 19E. 92.39%, 91.43% and 80.96% of
women were found to be positive for IgG, IgA and IgM, respectively. Therefore, with
respect to lack of protective anti-tetanus antibody in a minority of women, our results
suggest that this group might be at risk of tetanus disease and if they get pregnant,
need to be vaccinated against tetanus in order to produce sufficient Ab for their own
protection and to provide their fetuses with anti-tetanus antibody.
http://mjiri.iums.ac.ir/article-1-981-en.pdf
Antibody
tetanus
mother
fetus.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
313
318
article
TRA UMATIC ACUTE SUBDURAL HEMATOMA: ANALYSIS OF FACTORS AFECTING OUTCOME IN COMATOSE PATIENTS
SHIRZAD AZHARI
1
HOSEIN SAFDARI
2
MASSOUD SHABEHPOOR
3
HOSEIN NAYEBAGHAIE
4
ZOHREH AMIRI
5
From the Department of Neurosurgery, Imam Hosein Medical Center, Shaheed Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
To determine the factors affecting the outcome of patients with traumatic acute
subdural hematoma, we reviewed the records of7 4 consecutive comatose patients
with a Glascow coma scale Score (GCS) of less than 8 who had been admitted to
Imam Hosein Medical Center from 1990 to 1996 and had undergone a uniform
treatment protocol.
The overall mortality rate was 73% and 23% had functional recovery, but 4%
were severely disabled or vegetative. The following variables had a statistically
significant correlation with poor outcome: age over 65 years (p<0.05), preoperative
GCS of 3 or 4 (p<0.05), bilateral absent pupillary light reflexes (p<0.05), and
immediate and sustained coma from the moment of injury to operation without any
lucid interval (p<0.00l). The time interval between injury and operation, sex,
mechanism of injury, and associated craniocerebral injuries were not significantly
correlated with outcome. In patients with immediate post-traumatic
unconsciousness, the extent of primary brain injury is the crucial factor to predict
the outcome. However, in patients with a lucid interval, the mass effect of
hematoma seems to be more important, therefore prompt surgical decompression
in addition to management of secondary brain insults improves the outcome
remarkably.
http://mjiri.iums.ac.ir/article-1-980-en.pdf
Acute subdural hematoma
GCS
lucid interval
outcome
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
309
312
article
THE VALUE OF IMMUNOLOGICAL TESTS IN PREDICTING PREGNANCY OUTCOME
A A. REZAEI
1
From the Department of Immunology, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
In addition to ultrasonographic monitoring of folliculogenesis, urinary luteinizing
hormone testing, luteal phase endometrial biopsy, serum antiphospholipid
antibodies, etc., immunologic tests are recently being used to evaluate reproductive
disorders. In this study, sharing of HLA-A, B, C and DR antigens and the presence
of antipaternal cytotoxic antibody (APCA) was assessed using microcytotoxicity
method.
Subjects of the study were 103 infertile couples, 47 fertile couples with 2-3
children and 123 couples with recurrent spontaneous abortions (RSA).
The results showed that the presence of antipatemal cytotoxic antibody is
associated with fertility rather than infertility (p<0.0001), but sharing of HLA
antigens failed to show any significant difference between infertile and fertile
couples or couples who experienced spontaneous abortions compared with normal
couples. Therefore it appears that HLA antigen sharing is not associated with
pregnancy outcome and other antigen(s) may be involved in this process. Assessment
of APCA could be useful in predicting the pregnancy outcome.
http://mjiri.iums.ac.ir/article-1-979-en.pdf
APCA; HLA-antigen; RSA; fertile.
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1999-02
12
4
303
307
article
HEATER PROBE THERMOCOAGULATION A S A SUBSTITUTE FOR SURGICAL INTERVENTION TO ARREST MASSIVE PEPTIC ULCER BLEEDING: A CONTROLLED, PROSPECTIVE ANALYSIS OF 42 CASES
D SHARIFI DOLOUI
1
From the Department of Gastroenterology, Department of Internal Medicine, Ghaem Medical School,
The goal of this study was to compare the effect of heater probe thermocoagulation
for massive bleeding of peptic ulcers with a control group. Between March 1992 and
August 1995 we used heater probe thermocoagulation endoscopically to treat 42
patients with active UGl bleeding or nonbleeding visible vessels at the base of ulcer
craters within 2-3 hours of admission. We also selected 42 patients with active
bleeding or non bleeding visible vessels who did not receive any endoscopic
treatment but were instead treated conservatively as the control group.
The energy applied to each of our patients in the heater probe group was
105±22.5 J (mean±SD). Rebleeding occurred within 2-5 days in 2 patients (4.7%) in
the heater pro be group versus 9 patients (21.4 % ) in the control group (p= 0.05). Mean
duration of admission in the heater probe group was 4.3±3.1 days versus 6.9±3.8 days
in the control group which was comparable (p= 0.0027).
There were no statistically significant differences between the two groups
concerning transfusion requirement and mortality. Heater probe therapy was tolerated
by the patients very well and no complications occurred.
Heater probe thermocoagulation is an effective, safe and economical procedure
for treating peptic ulcer bleeding.
http://mjiri.iums.ac.ir/article-1-978-en.pdf
Peptic ulcer
Bleeding
Heater probe treatment.