eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
243
247
article
DEVELOPMENT OF A RAPID SPECTROPHOTOMETER SCREENING METHOD FOR QUANTITA TION OF ACETAMINOPHEN IN HUMAN SERUM
JALIL TAVAKOLI AFSHARI
1
TZAN LIU
2
From the Department of Laboratory Medicine, San Francisco State University, California, U. S.A.
We describe a simple, economical procedure for the emergency
determination of serum acetaminophen levels. Prior to color development,
free unconjugated acetaminophen was separated from other endogenous
compounds containing phenol groups by extracting the acetaminophen into
ethyl acetate and hydrolyzed to p-aminophenol by treatment with ho acid. A
blue color, which can be measured at 635 nm is formed by the addition of
2,5-dimethylphenol (p-xylenol) and sodium periodate, based on the reaction
of a primary aromatic amine and a phenolic hydroxyl reagent (Figure 1).
This method is linear from 25-600 mg/L. The intra-run precision had
day-to-day coefficient variation of between 4.8 and 7.0. The method was
correlated with established nitration method of Glynn and Kendal (1975)
and the colorimetric method of Liu and Oka (1980) with the correlation
coefficient of 0.97 and 0.98 respectively.
http://mjiri.iums.ac.ir/article-1-1256-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
237
242
article
INHIBITION OF14C UPTAKE BY CANDIDA ALBICANS: A NEW METHOD FOR INVESTIGATION OF INTRACELLULAR KILLING BY PHAGOCYTES
A A. GHAVAMI NEJAD
1
T ZANDIEH
2
From the Department of Biology, Faculty of Sciences, Isfahan University of Medical Sciences, Isfahan,
the Iranian Blood Transfusion Service, Tehran, Islamic Republic of Iran.
A radio assay employing 14C-uridine to measure the intracellular killing
of Candida albicans by phagocytes is presented.
The principle of this new method is that after killing of Candida albicans
by phagocytes RNA synthesis stops and therefore uridine uptake is diminished
whilst viable candida in cultures would take up uridine and as a result,
high CPM counts can be obtained within 135 min. of incubation in test
cultures. Leukocytes from 30 normal subjects showed reduced C.P.M.
following killing of Candida albicans.
http://mjiri.iums.ac.ir/article-1-1255-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
229
236
article
LOCAL PRODUCTION OF PRIMARY POWDER OF BACILLUS THURINGIENSIS SEROTYPE H-14 IN IRAN AND DETERMINATION OF ITS INSECTICIDAL PROPERTIES AGAINST CULEX PIPIENS AND ANOPHELES STEPHENSI
F FAYAZ
1
N MOASAMI
2
M ZAIEM
3
M MOTABAR
4
K HOLAKOUEI NAEINY
5
Iranian Research Organization For Science and Technology
School of Public Health and Institute of Public Health Research,
From the Microbiology Department, Faculty of Medicine, Shahid Beheshti University of Medical Sciences
and the Departments of Entomology and Epidemiology, Tehran University of Medical Sciences, Tehran,lslamic Republic of Iran.
Three media formulated from molasses and cornsteep liquor and
another media based on the basal medium formulation of IPS-82 were
assessed for the growth and production of insecticidal properties of B. thuringiensis
H-14 .• Bacterial powders prepared from the broth cultures were
assayed against the larvae of Culex pipiens and Anopheles stephensi. A
standard powder of IPS-82 was included in the assay for comparison. Good
. growth was obtained in all the media and all powders were effective against
the two types of mosquito larvae. Media with the basal medium formulation
of Institute Pasteur Paris were the most effective. Molasses III media was
also very favorable. The concentrations required to kill 50% of the larvae of
Culex pipiens and Anopheles stephensi for these two were as follows:
- Media with the basal medium formulation of Institute Pasteur Paris:
24hr. Lc50 and it's 95% confidence limits of Culex pipiens and Anopheles
stephensi = 0.0025,0.0031,0.0018 mg/lit
0.1792,0.2603,0.1508 mg/lit
- Molasses III Media:
24hr. Lc50 and it's 95% confidence limits of Culex pipiens and Anopheles
stephensi = 0.0103,0.0115,0.0092 mg/lit
0.1994,0.2223,0.1769 mg/lit
The investigation shows that these media can be used for the production
of B.thuringiensis H-14 primary powder.
http://mjiri.iums.ac.ir/article-1-1254-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
219
228
article
CYCLIC NUCLEOTIDES CONTROL DIFFERENTIATION OF HUMAN MONOCYTES INTO EITHER HIGHLY ACCESSORY CELLS OR MACROPHAGES
HM NAJAR
1
S RUHI
2
ANE CHRISTINE A.C. BRU-CAPDEVILLE
3
Prof. J.H. PETERS
4
From the Department of Immunology, University of Gottingen, West Germany
Human peripheral blood monocytes have been found to undergo a
transitory state of high accessory activity before they fully become macrophages.
Time kinetics were done to follow this accessory potential.
Studying the regulation of accessory activity, we have found that monocyte
derived accessory cells (m-AC) pass through two phases of development,
both of which are adversely controlled by cyclic nucleotides. Phase I is
positively correlated by intracellular cAMP increase and can be blocked by
increase of cGMP, whereas phase II positively correlates with increase of
intracellular cGMP and can be completel y blocked by cAMP and synergystic
agents. In addition to cAMP, non-cyclic adenine nucleotides and adenosine
also mimic all cAMP effects. This behavior is explained by the known
presence of surface 5- nucleotidase and adenosine receptors which in turn
leads to activation of adenyl ate cyclase. At phase II, serum is required to
convert m-AC into macrophages. In the absence of serum, cells were
arrested in the m-AC state. Adenine nucleotides effectively counteract
serum induction, leading to the development of m-AC even in the presence
of serum. Monocyte/macrophage markers such as Fc receptors and nonspecific
esterase strictly correlate negatively with the expression of accessory
activity, whereas morphologically the appearance of veils positively correlates
with all experimental situations of high accessory activity. Therefore, it
is evident that serum contains regulatory factors that strongly modify the
accessory potency of the m-AC via the cyclic nucleotide system, thus
presenting a new immunoregulatory principle at the beginning of the
immune cascade.
http://mjiri.iums.ac.ir/article-1-1253-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
213
218
article
SPECTROPHOTOMETRIC AND GAS CHROMATOGRAPHIC METHODS FOR DETECTION AND ASSAY OF MUSTARD GAS
A SHAFIEE
1
A CHERAGHALI
2
A KEBRIAEIZADEH
3
From the Department of Organic Chemistry, College of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
A colorimetric method for detection and assay of mustard gas in urine
after addition of thymolphthalein is reported. The detection limit was 80
ng/ml of llrine. Mustard gas could also be detected in urine after extraction
with gas chromatography using FID detector. The detection limit was 10
ng/ml.
In addition, thiodiglycol, a metabolite of mustard gas, could be
converted to mustard with concentrated HCl at 100°C and detected with gas
chromatography.
http://mjiri.iums.ac.ir/article-1-1252-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
207
212
article
The "Kitab al-Shukuk\'alas Jalinus" of Muhammad ibn Zachariya al- Razi
M MOHAGHEGH
1
http://mjiri.iums.ac.ir/article-1-1251-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
201
206
article
SURVIVAL IN PATIENTS WITH MALIGNANT GLIOMAS OF THE BRAIN
HOSSEIN SAFDARI
1
From the Neurosurgical Service, Imam Hossein Hospital, Shahid Beheshti University, of Medical Sciences, School of Medicine, Tehran, Islamic Republic of Iran.
The present retrospective study was designed to analyze factors with
prognostic values a) within, and b) significantly associated with, short-term
(12months or less) and long-term (more than 24 months) survival times, i n 72
consecutive patients treated for malignant gliomas. Among 41 (57%)
short-term surviving patients, the absence of both aphasia and motor deficit
(as initial presenting symptoms), postoperative radiation therapy alone or in
combination with chemotherapy, and reoperation were associated with a
relatively better survival prognosis. Among 15 (20.8%) long-term surviving
patients, postoperative radiation therapy and anaplastic, as histological type
of astrocytoma, were marginally associated with improved survival time.
The addition of conventional chemotherapy to postoperative radiation
therapy failed to show a significant improvement in survival time in both
survival groups. The long-term survival was significantly associated with the
following factors: age (under 51 years), reoperation and the absence of
lymphocyte infiltration.
A satisfactory quality and duration of survival was achieved in a
minority of patients with malignant gliomas. Furthermore, a more appropriate
postoperative antitumor therapy may increase the number of these
patients, by avoiding fatal injuries to the normal brain tissues induced by
postoperative antitumor therapies.
http://mjiri.iums.ac.ir/article-1-1250-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
197
200
article
MODIFIED ONE- FINGER LAPAROSCOPY TECHNIQUE
H DABIRASHRAFI
1
Y BEHJATNIA
2
N MOGHDAMI
3
From the Department of Obstetrics and Gynecology, Mirza kouchek khan (Women's) Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
We used the modified one finger laparoscopy technique on 100 patients
and found it to be successful in 87% of cases. No major complication
occurred during or after such operations. However, we think that this
technique is accompanied by three important problems:
1) The CO2 gas is lost f1"om the subumbilical incision,
2) A secondary fascial layer is present in some patients, and
3) There is a higher rate of laparoscopic wound complications.
At the present time (in the second phase of the study), by cutting
superficially the secondary fascial layer , our success rate has risen to 98%.
We close the fascia and skin in separate layers and believe that a similar
approach can be used for the peritoneum.
http://mjiri.iums.ac.ir/article-1-1249-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
193
196
article
GRAM NEGATIVE BACILLI IN BURNS
TAIMOUR YOUSEF LANGAEE
1
KIUMARS GHAZI SAIDI
2
YAHYA DOWLATI
3
From the Department of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
In a period of nine months, 225 strains of Gram-negative bacilli isolated
from burns were identified by a variety of tests in two burn centers in Tehran.
The most common species were Pseudomonas aeruginosa, Pseudomonas
fluorescens, Escherichia coli, Enterobacter agglomerans, Enterobacter
cloacae and Klebsiella pneumoniae. Many strains of acinetobacter spp,
providencia spp, proteus spp, serratia spp, and Citrobacter freundii were also
isolated.
All the strains were tested for sensitivity to bacitracin, carbenicillin
colistin, cephalothin, chloramphenicol, gentamicin, kanamycin, lincomycin,
trimethoprim and tetracycline. The proportion of strains sensitive and
resistant to different antibacterial agents varied widely with species of
bacteria. Most of the strains except pseudomonas spp were sensitive to few of
the antibacterial agents and posed no problem with relation to treatment.
Pseudomonas spp. were 100% sensitive to polymyxin B and colistin
(polymyxin E ) and 90% resistant to gentamicin and carbenicillin.
http://mjiri.iums.ac.ir/article-1-1248-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
189
192
article
OUTPATIENT RETINAL DETACHMENT SURGERY: A PRELIMINARY REPORT
M MODARRES ZADEH
1
From the Dept. of Ophthalmology, LabbafineJad Medical Center, Shahid Beheshti UniversiIy of Medical Sciences, Tehran, Islamic Republic of Iran.
Retinal detachment surgery has been considered an inpatient hospital
procedure requiring preoperative and postoperative hospitalization. In this
pilot study, outpatient retinal detachment surgery was performed on sixteen
patients and the results (100% success rate after an average follow-up of 8.3
months) were comparable or better than reported overall success rates for
retinal detachment in the literature.
This prospective and non-randomized study, although small in scale,
suggests that ambulatory retinal detachment surgery is safe and successful.
This is the first report on outpatient retinal detachment surgery in world
ophthalmic literature.
http://mjiri.iums.ac.ir/article-1-1247-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
179
184
article
PERCUTANEOUS TRANSTHORACIC BIOPSY USING TRU-CUT NEEDLE
FARHOOD SAREMI
1
From the Department of Radiology, Vali-Asr Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran
Biopsies of thoracic lesions guidance. 22 of the 1 12 lesions were mediastinal and 83 were pleuroparenchymal.
A large-bore 14-gauge Tru-cut needle was used in all patients.
Adequate specimens for histologic examination were obtained in 98%. The
overall accuracy was 91 % with 9% false negative and no false positive
diagnoses. Despite the large size of the needle, the total number of
complications remained low ( 17%). Pneumothorax was detectable in 1 1.5%
of biopsies and hemoptysis in 2.6%. Tru-cut needle is suitable for biopsying
chest wall tumors, pleural based lesions, superior sulcus tumors, and
accessible mediastinal lesions. The instrument is useful not only for diagnosing
malignacy but also for determining specific malignant cell types and
diagnosing benign disorders.
http://mjiri.iums.ac.ir/article-1-1246-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
175
178
article
IMPORTANT DIAG NOSTIC AND SURGICAL POINTS IN HORSESHOE KIDNEY SURGERY
NASSER SIMFOROOSH
1
REZA MAHDAVI
2
From the Department of Urology, Shahid Labbafi Nejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
Prom April, 1983 until December, 1987, 15 patients with horseshoe
kidneys underwent surgery in Shahid Labbafi Nejad Medical Center.
Pyelolithotomy was done in 11 cases, pyeloplasty in 2 cases, and nephrectomy
in 2 cases. Symphysiotomy was performed in 14 of these patients.
Preoperative angiography was not done in any of the cases and midline
transperitoneal approach was utilized in all of the cases. We consider
angiography an unnecessary invasive procedure in the great majority of the
horseshoe kidney operations. Midline transperitoneal approach is far
superior to flank approach in horseshoe kidney surgery. We also prefer
symphysiotomy in these cases, since it improves drainage from horseshoe
kidney pelvices.
http://mjiri.iums.ac.ir/article-1-1245-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
171
174
article
LATE COMPLICATIONS OF SULFUR MUSTARD IN RESPIRATORY SYSTEM
H SOHRABPOUR,
1
MR MASJEDI
2
M BAHADORI
3
In the last few years many chemically injured patients have been visited
throughout the country in order to investigate the late respiratory complications
of sulfur mustard. 35 patients who were previously admitted for
mustard poisoning underwent complete clinical examination as well as
spirometry, fiberoptic bronchoscopy (FOB), Bronchoalveolar lavage
(BAL), and trans bronchial lung biopsy (TBLB).
The results of this study reveal a high incidence of air flow obstruction in
these patients (50% ).BAL shows decreased numbers of macrophages and
increased numbers of lymphocytes and neutrophils, and the presence of
eosinophils (between 1 to 5%). Certain pathologic features have been
encountered. We conclude that late respiratory complications of sulfur
mustard are major causes of morbidity and are quite resistant to therapy.
http://mjiri.iums.ac.ir/article-1-1244-en.pdf
eng
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
2251-6840
1988-11
2
3
165
170
article
REPORT OF 200 RENAL TRANSPLANTATIONS FROM LIVING DONORS
IRADJ FAZEL
1
IRADJ GHODOUSI
2
From the Department of Vascular Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
Two hundred cases of renal transplantation from living donors are
reported from Shahid Hashemi Nejad Medical'Center in Iran. There were
127 male and 73 female patients with 67% in the third and fourth decade of
life. All patients received grafts from living donors with 1 14 cases of HLA
haploidentical, 55 cases of HLA identical matches and 24 unrelated donors.
One year patient and graft survival were 94. 3% and 88.6% respectively.
Despite the wartime conditions and lack of many modern facilities, the
results are quite satisfactory and comparable to many other centers in the
world. It seems more logical to replace costly chronic hemodialysis by renal
transplantation programs in the Middle East and North African countries
through planned and cooperative programs.
http://mjiri.iums.ac.ir/article-1-1243-en.pdf