CASE_STUDY
A NEONATAL PRESENTATION OFCAROLI\'S DISE ASE WITH SEVERE ABDOMINAL DISTENTION AS A PRESENTING SYMPTOM
In this article , the authors introduce a case of Caroli's Disease (CD) according to
the results of ultrasonography (U.S), abdominal computed tomography (CT scan) and
hepatic biopsy that has been manifested in the neonatal period. In the routine examination
of the neonate, abdominal protrusion was noticed. Then milk intoterance, inability
of meconium passage and vomiting developed, during workup, a diagnosis of CD was
confirmed. Although CD is rare in the neonatal period, it should be considered in the
differential diagnosis of abdominal protrusion.
http://mjiri.iums.ac.ir/article-1-643-en.pdf
2004-09-15
185
189
Caroli\'s Disease
Caroli syndrome
Intrahepatic bile duct dilation
Abdominal protrusion
Neonate
A
A. BAZRAFSHAN
1
From The Department of Pediatrics and Pediatric Surge/y, Ghaem Medical CenteJ; Mashhad University of Medical Sciences, Mashhad.l.R. Iran
AUTHOR
M
OMIDIAN
2
AUTHOR
CASE_STUDY
PEACILOMYCES INFECTION IN AN IMMUNOCOMPROMISED PATIENT
Paecilomyces sp, is a saprophytic fungus which has rarely been associated with
human disease. We report the first case of invasive subcutaneous infection caused by
this fungus in a 78-year-old female from Damghan, Iran, with diabetes and chronic
renal failure. A biopsy provided an initial diagnosis of the mold in tissue. Multiple positive
fungal cultures which were obtained from the biopsied tissue were subsequently
identified by microscopic and macroscopic characteristics to be Paecilomyces sp.
This case was successfully treated by oral ketoconazole (200 mg/day).
http://mjiri.iums.ac.ir/article-1-642-en.pdf
2004-09-15
181
184
Paecilomyces infection -fungal infection - immunocompromised patients.
SHAHINDOKHT
BASSIRI JAHROMI
shahindokhtbassiri@yahoo.com
1
From the Department of Medical Mycology, Pasteur Institute of Iran, Tehran, Iran
AUTHOR
ALI ASGHAR
KHAKSAR
2
AUTHOR
CASE_STUDY
THE APPENDIX, AN ACCEPTABLE SUBSTITUTE FOR ALL SEGMENTS OF BOTH URETERS: A REPORT OF TWO CASES
There are occasional reports of using the appendix to replace sections of the
ureters, and to our knowledge, only a couple of them were in the left side.
We used the appendix as a ureteral substitute in two cases in technically difficult
parts, the upper left ureter and the first part of the right ureter.
Follow-ups of8 years and 6 months in right and left side cases, respectively, are
impressive with maintenance of patency and improving renal function.
We believe that the use of the appendix is a valuable alternative to substitute all
segments of both ureters, with good results.
http://mjiri.iums.ac.ir/article-1-640-en.pdf
2004-09-15
177
180
Ureter
Injury
Replacement
Appendix Vermicularis.
MA
ZARGAR
1
AUTHOR
M
MIRZAZADEH
mjmirza@iums.ac.ir
2
From the Dept. of Urology. Hashemi Nejad Hospital. Iran University of Medical Sciences. Tehran.IR. Iran.
AUTHOR
K
ZARGAR
3
AUTHOR
CASE_STUDY
A CASE REPORT OF VASCULAR COMPLICATIONS, HEMOP TYSIS AND COR PULMONALE IN BEHCET\'S DISEASE
A 33-year-old female-a known case of Behcet's disease- was admitted to the
hospital due to fever, dyspnea and hemoptysis. One week before admission, she had
developed right leg swelling. The history suggested either deep vein thrombosis (DVT)
and pulmonary emboli or pulmonary artery vasculitis. Diagnostic tests were requested
and the necessary treatment was started accordingly
http://mjiri.iums.ac.ir/article-1-639-en.pdf
2004-09-15
173
176
Behcet\'s disease - Vascular complication - Thrombosis - Vasculitis.
ALI
KHALVAT
1
From the Dept. of Rheumatology, imam Khomeini Hospital. Tehran University of Medical Sciences. Tehran. l.R. Iran
AUTHOR
REZA
NAJAFIZADEH
2
AUTHOR
ORIGINAL_ARTICLE
PURIFICATION AND CHARACTERIZATION OF CELL WALLMANNOPRO TEINS OF CANDIDA ALB/CANS USING INTACT CELL METHOD
Virulence of the opportunistic yeast, Candida albieans, involves the interplay of
many complex changes including the yeast-hyphae transition, which mainly involves
protein changes. Cell wall mannoproteins are found to be the main cause of adherence
of C. albieans to epithel ial cells in the first step of an infection process. In the present
study, cell wall mannoproteins of intact yeast were purified using a simple treatment of
yeast with mercaptoethanol and sodium dodecyl sulfate followed by Concanavalin A
chromatography. Both electrophoretic analysis of the column effluent and Western blot
analysis using polyclonal and monoclonal antibodies showed the presence of
mannoproteins with molecular weight in the range of30-50 kDa. Dot blot analysis of
the purified antigen with the polyclonal and monoclonal antibodies prepared in this
study showed that outer membrane mannoprotein antigens were obtained successfully
following the above simple purification strategy
http://mjiri.iums.ac.ir/article-1-638-en.pdf
2004-09-15
167
172
Candida albicans
Mannoproteins
Purification
Characterization
Z
FARAHNEJAD
1
From the Department 0f Medical Mycology
AUTHOR
MJ
RASAEE
rasaee _m@modares.ac.ir.
2
Department of Clinical Biochemistry
AUTHOR
H
YADEGARI
3
From the Department 0f Medical Mycology
AUTHOR
M
FROUZANDEH MOGHADAM
4
Department o/Medical Biotechnology, School of Medical Sciences, Tarbiat Modarres University, Tehran. I.R. Iran
AUTHOR
ORIGINAL_ARTICLE
IN VITRO INHIBI TI ON OF ATTACHMENT OF T HE AMERICAN TRYPANOSOME, TRYPANOSOMA RANGELI ON THE SALIVARY GLAND OF I TS VECTOR, RHODNIUS PROLIXUS, USING THE PARASITE EXTRACT AND PARTI AL PURIFICATION OF AN ATTACHMENT INHIBITOR PROTEIN
The life cycle of the American trypanosome, Trypanosoma rangeli, in its invertebrate
host, Rhodnius prolixus, is completed by invasion into the insect salivary glands.
As a prerequisite, there is an initial recognition of the salivary gland receptors for the
adhesion/invasion by T rangeli. Therefore, the purpose of this study was to investigate
the putative receptor molecules on either the salivary gland or parasites surfaces,
which mediate the adhesion/invasion. Initially, the possible agglutination activity ofT
rangeli epimastigotes against blood cell was examined. Then, in order to investigate
the role of any receptor ligands on the surface of T rangeli in adhesion/invasion of the
parasite, GlcNAc was preferentially used to isolate a ligand protein. A 60kDa protein
bound to the GlcNAc column and was isolated from T rangeli (Tr-60) and the purity
of this protein was confirmed by reverse phase HPLC. Tr-60 also exhibited a potent
parasite inhibitory effect indicating that this protein (most probably a glycoprotein) may
act as a ligand receptor. In vivo, the development and invasion of T rangeli into the
salivary gland was not interfered with by the presence of a sugar inhibitor of the R.
prolixus hemolymph lectin
http://mjiri.iums.ac.ir/article-1-637-en.pdf
2004-09-15
159
166
Tlypanosoma rangeli
Rhodnius prolixus
Salivary glands
P roteins
attachment.
HR
BASSERI
hamid_basseri@hotmail.com" hamid_basseri@hotmail.com
1
the Department of Medical Entomology. School of Public Health, Tehran University of Medical Sciences, P0. Box 14155-6446. Tehran, Iran
AUTHOR
NA
RATCLIFFE
2
Biomedical and Physiological Research Group, School o/Biological Sciences, University o/Wales Swansea, Singleton Park. Swansea SA2 8PP United Kingdom
AUTHOR
ORIGINAL_ARTICLE
STIMULATORY EFFECT OF NIGELLA SATIVA ON β2-ADRENOCEPTORS OF GUINEA PIG TRACHEAL CHAINS
Our previous studies have demonstrated the relaxant, anticholinergic (functional
antagonism), antihistaminic, and inhibitory effect of calcium channels of Nigella sativa
on guinea pig tracheal chains. To investigate the other mechanism responsible for the
relaxant effect of this plant, the stimulatory effect of Nigella sativa on J32-adrenergic
receptors in tracheal chains of the guinea pig was examined in this study.
The 2-Adrenergic effects of macerated and aqueous extracts from Nigella sativa,
50 nM propranolol, and saline were tested by performing the cumulative Log
concentration-response curves of isoprenaline induced relaxation of precontracted isolated
guinea pig tracheal chains with three different conditions including: non-incubated
(group 1, n=8) incubated with I ).lM chlorpheniramine, and 1 ).lM atropine (group 2,
n=5). The effective concentration of isoprenaline, causing 50% of maximum response
(ECso)' maximum response and the slope of isoprenaline curves obtained in the presence
of extracts, and propranolol were compared with those of saline.
The results showed clear leftward shifts in isoprenaline curves obtained in the
presence of both macerated and aqueous extracts compared with that of saline in group
2. The EC50 obtained in the presence of aqueous extract in group 2 was significantly
lower than that of saline (p<0.05). The maximum response obtained in the presence of
aqueous extract in group 2 was non-significantly greater than that of saline. However, in
group 1 experiments there was no significant difference between EC50 and maximum
responses obtained in the presence of two extracts and saline, although isoprenaline
curves obtained in the presence of both macerated and aqueous extracts showed clear
rightward shifts compared to that of saline. Isoprenaline curves were obtained in the
presence of propranolol in both groups of experiments. The EC50 obtained in the
presence of propranolol was significantly greater than that obtained in the presence of
saline and extracts in both groups of experiments (p<0.00 1 for all cases).
The results of this study indicated a stimulatory effect of aqueous extract and a
possible stimulatory effect of macerated extract from Nigella sativa on β2-
adrenoceptors.
http://mjiri.iums.ac.ir/article-1-636-en.pdf
2004-09-15
153
158
Nigella sativa
adrenergic effect
trachea
guinea pig
MH
BOSKABADY
mhboskabady@hotmail.co m
1
From the Dept. of Physiology, Ghaem Medical Centel Mashhad University of Medical Sciences, Mashhad, l.R. Iran
AUTHOR
S
KIANI
2
AUTHOR
P
JANDAGHI
3
AUTHOR
ORIGINAL_ARTICLE
TELOMERASEACTIVITYIN IRANIAN PATIENTS WITH ESOPHAGEAL SQUAMOUS CELL CARCINOMA
Telomerase activation is one of the main pathways to immortalize cancer cells. In
many kinds of cancer cells, this special reverse transcriptase stabilizes and elongates
telomeres and prevents telomere erosion that naturally occurs in every cell division.
Esophageal cancer is the fifth most frequent cause of cancer death worldwide, and is
highly associated with alcohol, smoking, cultural habits, and environmental factors.
Telomerase has been suggested as a tumor marker and a molecular target for drug
design in several kinds of cancers. In this work telomerase activation was inspected
among Iranian patients with Esophageal Squamous Cell Carcinoma (ESCC), and detected
in 90% of samples of different stages. This may be an indication that telomerase
activation happens in an initial step in the development of ESCC. Although there is no
correlation between telomerase activity and the progress of ESCC, it could be considered
as a good tumor marker in ESCC. Telomerase activity tests are suggested for
screening purposes in high risk areas for ESCC, which can be easily done on a small
amount of scrapped samples of esophageal mucosa. It is also possible that ESCC
results from incomplete differentiation or a failure in telomerase gene switching off that
nonnally occurs during the differentiation of esophageal epithelial cells.
http://mjiri.iums.ac.ir/article-1-635-en.pdf
2004-09-15
147
152
telomerase
telomere
esophageal squamous cell carcinoma
TRAP assay.
S
KAZEMI NOUREINI
1
From the *Institute of Biochemistry and Biophysics. P 0. Box: 13145-1384. University of Tehran. Tehran.
AUTHOR
AA
ZIAEE
aa_ziaee@yahoo.co.uk.
2
From the *Institute of Biochemistry and Biophysics. P 0. Box: 13145-1384. University of Tehran. Tehran.
AUTHOR
F
RASTEGAR-JAZlI
3
National Research Center for Genetic Engineering and Biotechnology (NRCGEB). Tehran
AUTHOR
MY
AZDANBOD
4
Department of Surgery. Shariati Hospital. Tehran University o/Medical Sciences. Tehran. Iran.
AUTHOR
ORIGINAL_ARTICLE
IMPROVEMENT OF hGM-CSF EXPRESSION USING A FUSION SYSTEM BY DIRECTING PROTEIN TO THE PERIPLASMIC SPACE
Bacterial expression systems provide economic and logistic advantages in manufacturing
proteins for human therapeutic purposes. However, most such proteins accumulate
in insoluble biologicaIIy inactive form when overexpressed in bacterial cells. This
is the case while attempting to produce recombinant human granulocyte-macrophage
colony stimulating factor (hGM-CSF) in E. coli. In this report, different strong promoters
are used to compare the yield of expression when the protein is expressed as
insoluble and soluble forms. The results obtained indicated that the level of expression
was independent of the nature of promoter used. Moreover experimental data presented
here suggest that the fusion system which directs hGM-CSF to the periplasmic
space not only can prevent inclusion body formation but also improve the level of
expression significantly.
http://mjiri.iums.ac.ir/article-1-634-en.pdf
2004-09-15
141
146
Escherichia coli
hGM-CSF
Expression
Inclusion body
Fusion protein.
MANA
OLOOMI
Oloomi@institute.pasteur.ac.ir
1
From the Molecular Biology Unit, Pasteur institute of Iran Tehran, l.R. Iran.
AUTHOR
SAEID
BOUZARI
2
AUTHOR
ORIGINAL_ARTICLE
SURVEY OF TEN CASES OF EARLY I NFANTILE EPILEP TIC ENCEPHALOPATHY (OHTAHARA SYNDROME)
Early infantile epileptic encephalopathy or EIEE (Ohtahara syndrome OS) is a
kind of intractable seizure that begins in neonatal age with sudden onset of tonic spasms
in series or single suppression-burst S-B in EEG.I Imaging shows anatomic defects
such as migration disorders and generalized atrophy" with essentially normal metabolic
tests. The seizures often change to West's syndrome (WS) or Lennox G austaut syndrome
(LG S) pattern.3 Ohtahara et a1. first described this syndrome in 1976.3 We
observed ten cases of this syndrome during a period of I 2 months. 70% of the patients
were male. The mean age at the time of onset of seizures was 8 days the mean age at
the time of admission was 6.8 months. In 20% of cases the first seizures were generalized,
in 80% tonic. EEG at the time of referring showed suppression-burst S-B in 50%,
hypsarrhythmia in another and in 60% of them there were scattered sharp, spike or
spike and wave in one or both sides. The drugs that have been used in mUltiple therapy
were clonazepam 50%, prednisone 60%, sodium valproate 30%, nitrazepam 20%,
vigabatrin 30% and acetazolamide in 10%. Response to treatment was good in 70%
and moderate in 30%. At the end of the course all patients showed severe mental and
motor retardation. Prognosis was shown to be poor in these patients.
http://mjiri.iums.ac.ir/article-1-633-en.pdf
2004-09-15
135
139
Early infantile epilepsy with suppression-bursts
EIEE; Intractable infantile seizure
Ohtahara syndrome
OS; Early myoclonic encephalopathy
A
A. NASIRIAN
1
From the Department of Pediatric Neurology, Children S Medical Center Tehran University of Medical Sciences. Tehran, I. R. Iran.
AUTHOR
ORIGINAL_ARTICLE
EMBOLECTOMY FORACUTE LOWER LIMB ISCHEMIA
In this prospective descriptive study, all patients with acute lower limb ischemia
who presented to Shohada Tajrish Hospital (STH) between July 200 1 and July 2003
were analyzed and followed for 6 months. The total population of 109 patients, with no
significant difference in sexual distribution had a mean age of 64± 16 years. 23% had
AF and 31 % had a history of MI, 'but in the ECG of 30%, no pathologic finding had
been detected. Pain and coolness were the two most prevalent symptoms. Only onethird
of the patients.had normal sensory and motor examination in their limb and the rest
had different degrees of impairment. More than 70% of the patients arrived here after
12 hours from the onset of the symptoms. Fasciotomy was done in 29%, and amputation
in 23.5%. The mortality rate was 12.2%. Surgical site infection occurred in 11 %
and] 4.1 % underwent re-embolectomy. There was no pseudoaneurysms. The time
interval from the onset, sensory and motor findings, and calf tenderness affected the
prognosis. Prognosis was better in smokers. The iatrogenic group which consisted of
13.7% of our patient population had the best prognosis (no mortality or morbidity). 4%
of the patients who had first undergone embolectomy in other centers, all lost their
limbs. In cases of late embolectomies, venotomy and heparin flush has lowered the
amputation level without increasing mortality. Early referral by a cardiologist, receiving
heparin rapidly and smoking are among the factors which improve prognosis.
http://mjiri.iums.ac.ir/article-1-632-en.pdf
2004-09-15
131
134
Acute ischemia; Embolectomy; lower limb.
M
MOZAFFAR
mohamad-mozafar@yahoo.com
1
From Shohada Tajrish Medica! Center, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran
AUTHOR
A
AFSHARFARD
2
AUTHOR
F
MALEKPOUR
3
AUTHOR
R
VAGHARDOOST
4
AUTHOR
ORIGINAL_ARTICLE
TRANSNASAL ENDOSCOPIC APPROACH FOR SINONASAL INVERTED PAPILLOMA
Inverted papillomas of the nose and paranasal sinuses are uncommon neoplasms,
characterized by their tendency to occur and by their association with malignancy. In
the past decade there has been a trend toward the use of endoscopic surgical techniques
in the management of these tumors. This article presents the results of a prospective
study of24 patients with inverted papilloma resected by the endoscopic approach.
21 patients had unilateral disease and three patients had bilateral involvement.
None of the patients had orbital or cranial extension. No association with malignancy
was seen.
Following endoscopic surgery, four patients had recurrences that required surgery.
No complication occured in any of the patients. The results of endoscopic surgery
for inverted papilloma is much better than nonendoscopic transnasal approaches and
compares favorably with radical extranasal approaches.
Endoscopic surgery is an effective surgical option for inverted papilloma removal.
http://mjiri.iums.ac.ir/article-1-631-en.pdf
2004-09-15
127
130
Inverted Papilloma
Endoscopic Surgery
Paranasal Sinus Neoplasm.
MOHAMMAD HOSSEIN
BARADARANFAR
baradaranf@hotmail.com.
1
From the Department of Otolaryngology, Head and Neck Surgery. Sadoughi Hospital, Yazd University of Medical Sciences, Yazd, l.R. Iran
AUTHOR
PAYMAN
DABIRMOGHADDAM
2
AUTHOR
ORIGINAL_ARTICLE
MUMPS MENINGOENCEPHALITIS IN PEDIATRIC WARD OFRASOOLAKRAMHOSPITAL IN TEHRAN, IRAN, 1999-2000
To determine the frequency of mumps infection in children hospitalized with aseptic
meningoencephalitis ( ME), as well as its correlation with parotiditis, this case-series
study was conducted in the pediatric ward ofRasool Akram Hospital in Tehran, Iran,
from 1 999 to 2001.
T he blood samples of these patients were tested for specific IgM antibody for
mumps by Elisa method. The results of this study showed that 62.7% of children with
ME were IgM positive, and the remaining (37.3%) were IgM negative. There was a
significant correlation between positive IgM and the presence of parotiditis. However,
no correlation was noted between age, sex and season with IGM positivity except for
CSF changes (aseptic meningitis) between the two groups. It can be concluded that the
presence of neural symptoms (especially aseptic meningitis) in children less than 7 years
of age with parotiditis could indicate mumps ME, especially in the spring. Due to a
higher percentage of unvaccinated young persons « 15yr old) in Iran, probably the
incidence rate of mumps infection and its sequelae are higher compared with its incidence
in developed countries before massive vaccination. Therefore with massive vaccination
ofIranian young people « 20yr old), the costs of mumps infection and its
sequelae will decrease.
http://mjiri.iums.ac.ir/article-1-629-en.pdf
2004-09-15
123
126
Mumps infection; Meningoencephalitis; Mumps vaccination.
SAMILE
NOORBAKHSH
saminoor@hbi.dmr.or.ir
1
From the Department of Pediatrics. Rasool Akram Hospital. Iran University of Medical Sciences,. Tehran,l.R.lran.
AUTHOR
FARZANEH
ASHTIANI
2
AUTHOR
SHAHNAZ
RIMAZ
3
AUTHOR
MASOMEH
BAKHSHAYESH
4
AUTHOR
ORIGINAL_ARTICLE
THE PREVALENCE OF ANTIPHOSPHOLIPI D SYNDROME IN PATIENTS WI TH RECURRENT PREGNANCY LOSS: A REPORT FROM SOUTH OF IR AN
In order to determine the role of different anti-phospholipid antibodies as an etiologic
factor in recurrent pregnancy failure, a prospective study was done on one-hundred
and thirty-eight women who had unexplained recurrent pregnancy loss (group I)
with one-hundred well-matched women with normal reproductive outcome allocated
as control group (GIl).
Sera from 138 patients and 100 controls were analyzed for anticardiolipin antibody
(ACLA) and lupus anticoagulant (LA). ACLA was measured by Elisa and LA
by activated PTT.
Sixteen women (1l .6%) had positive ACLA in group I, while 3 (3%) of group II
were positive for this antibody (p= 0.0 157 and odds ratio = 4.24).
LA was positive in 12(8.7%) of group I and 3(3%) of group II, but the difference
was not significant (p= 0.074, odds ratio=3.08).
Overall 24 women (17.4%) were positive for one of the mentioned antibodies
(p= 0.00055, OR= 6.81). Four patients were positive for both antibodies.
This study emphasizes the relationship between antiphospholipid syndrome and
recurrent pregnancy failure
http://mjiri.iums.ac.ir/article-1-628-en.pdf
2004-09-15
119
121
Anticardiolipin antibody
lupus anticoagulant
pregnancy loss
J
ZOLGHADRI
1
From the Department of Gynecology and Immunology. Shiraz University of Medical Sciences. Shiraz. Iran.
AUTHOR
B
GHARESI-FARD
2
AUTHOR
ME
PARSANEZHAD
3
AUTHOR
S
ALBORZI
4
AUTHOR
ORIGINAL_ARTICLE
EFFECTS OF DESMOPRESSIN ON MEMORY DISORDERS DUE TO ELECTROCONVULSIVE THERAPY (ECT) IN HUMANS
Electroconvulsive therapy (ECT) is an efficient treatment for several neuropsychiatric
disorders however a large number of patients develop memory impairment after
ECT. Different studies both on animals and human suggest that vasopressin has positive
effects on memory and improves cognitive functions. In this randomized, double-blind
controlled clinical trial, 50 patients with psychiatric disorders who were candidate for
ECT were studied. In the control group (20 patients) who received normal saline, ECT
resulted in impairment in several aspects of memory such as: immediate memory, short
tenn memory, visual memory, associate learning and memory quality. The sub-scores
for most of the sub-fractional aspects of memory by WMS tests were decreased significantly
by the 2nd and the 3rd tests (p<0.00 I for most of the measured parameters).
In the case group (30 patients), each patient received 60 Mg/day (in 3 doses)
desmopressin as intranasal spray during the ECT treatment most of the measured
parameters were increased significantly by the 2nd and the 3rd WMS tests. There
were significant differences between the two groups for most memory scores
(p<0.000 I). The data demonstrate that desmopressin has protective and facilitating
effects on memory impairment after ECT. Therefore, we conclude that desmopressin
may be effective in the prevention of memory disorders by facilitating effects on memory
and learning processes and may be used in patients who receive ECT for neuropsychiatric
disorders.
http://mjiri.iums.ac.ir/article-1-627-en.pdf
2004-09-15
111
117
Memory disorders
ECT
desmopressin
vasopressin.
M-AL-R
HADJZADEH
1
From the Department of Physiology and Avicel1l1a Psychiatric Hospital. Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
E
ABDOL LAHIAN
2
AUTHOR
MR
SARGOLZAIE
3
AUTHOR
MD
MOHEBBY
4
AUTHOR
ORIGINAL_ARTICLE
CORRECTED QT DISPERSION IN CHILDREN WITH ISOLATED UNCOMPLICATED MITRAL VALVE PROLAPSE
Mitral valve prolapse (MVP) is a relatively frequent valvular abnormality. In both
children and adults with MVP, an increased incidence of ventricular arrhythmias has
been reported. QT dispersion, defined as the difference in duration between the longest
QT interval and the shortest one, for a given set of electrocardiographic leads has
been proposed as a sign of regional difference in cardiac repolarization and as a marker
of increased risk of ventricular arrhythmias. This study was designed to compare the
corrected QT dispersion (QTD) in children with isolated uncomplicated prolapse with
a normal age-and sex-matched group of children. Twenty children with MVP (mean±SD
age: 8 ±5 years) and thirty normal children enrolled in the study. Corrected QT dispersion
was manually calculated on their surface electrocardiogram in all of them. QTD
was 3 9± 14.7 and 40± 15.2 milliseconds respectively. An unpaired Student t -test was
performed . There was no significant difference between the mean value of the two
groups (p value<O.O 1). The findings of our study, besides the reports of increased QT
dispersion in adults, suggest that increased QT dispersion in patients with MVP is a
time-evolved phenomenon and does not exist from the very early years of age.
http://mjiri.iums.ac.ir/article-1-626-en.pdf
2004-09-15
107
110
Corrected QT dispersion
Mitral valve prolapse
Children.
ELAHEH
MALAKAN RAD
malakan_rad@Kaums.ac.ir
1
From the Department of Pediatrics, Kashan University of Medical Sciences. Kashan, !.R. Iran.
AUTHOR
NADER
MOMTAZMANESH
2
AUTHOR
ORIGINAL_ARTICLE
THE RELATIONSHIP BETWEEN OXIDATIVE STRESS AND THE ONSET OF CORONARY ARTERY DISEASE
Oxidation of low density lipoprotein (LDL) particles plays a key role in the etiology
of atherosclerosis and coronary artery disease (CAD). Oxidative stress enhances
the likelihood of LDL oxidation and atherosclerotic plaque development. Paraoxonase
(PONI) is an enzyme associated with HDL that metabolizes organophosphates and
has antioxidant activity.
In order to investigate the relationship between oxidative stress and the onset of
coronary artery disease (CAD), total ferric reducing antioxidant power (FRAP)-as a n
index o f antioxidant capacity of plasma- and the activity of PON 1 were measured in
80 patients over 65 and 80 patients less than 55 years old as late and early-onset CAD
groups respectively. Plasma lipids were also determined.
Patients with early-onset CAD had significantly lower serum levels of HDL-C
(p<0.05) and higher LDL-CIHDL-C (p<0.01) than the late-onset group. This may
imply the significance of HDL at the onset of CAD. There was no difference in serum
levels of TG, TC, LDL-C, PON 1 activity and FRAP values between the two groups.
The FRAP value was significantly lower than the reference range for healthy subjects in
our laboratory. Although the FRAP value is lower in normal elderly people compared
to the younger subjects, there was no difference between the two groups. This indicates
that in young CAD patients, oxidative stress may be more important than in the
elderly subjects and should be monitored in conjunction with routine lipid measurements.
http://mjiri.iums.ac.ir/article-1-625-en.pdf
2004-09-15
101
105
Atherosclerosis
antioxidant
lipid peroxidation
oxidative stress
oxidized LDL.
F
NABATCHIAN
1
From the Department of Biochemistry, Tehran University of Medical Sciences, Tehran
AUTHOR
SH
KHAGHANI
2
From the Department of Biochemistry, Tehran University of Medical Sciences, Tehran
AUTHOR
R
BAGHERIAN
3
From the Department of Biochemistry, Tehran University of Medical Sciences, Tehran
AUTHOR
R
MIRI
4
Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran
AUTHOR
M
MAHMOODI
5
Amiraalam Hospital, Tehran University o/Medical Sciences, Tehran, Iran
AUTHOR
P
PASALAR
Pasalar@sina.tums.ac.ir
6
Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Phone: 98 (21) 611-2341
AUTHOR
ORIGINAL_ARTICLE
THE MUTATIONS OF RET PROTO-ONCOGENE IN MEDULLARY THYROID CARCINOMAS IN IRAN
MeduIIary thyroid carcinoma (MTC) occurs both sporadically and in the autosomal
dominantly inherited multiple endocrine neoplasia (MEN) type 2 syndromes. The
distinction between true sporadic MTC and a new mutation familial case is important
for future clinical management of both the patient and family. The susceptibility gene for
hereditary MTC is the RET proto-oncogene. DNA analysis for germline mutations of
the RET proto-oncogene was performed in a series of 24 patients with MTC [apparently
sporadic MTC (20 cases), familial MTC (2 cases), MEN 2A (one case) and
MEN 2B (one case)] to determine whether they were true sporadic cases or hereditary
forms. Genomic DNA was amplified using polymerase chain reaction (PCR) and
oligonucleotide primers for exons 10 & I 1. The PCR products were examined by
restriction enzymes analysis to detect the mutations. One of the 20 patients with apparent
sporadic MTC had exon 10 mutation (Cys-620 Arg) and exon I I mutation (Cys-
634 Trp) was also found in the index case with MEN 2A. No mutation was detected in
the other patients. Three of six evaluated members of the MEN 2A patient had the
same mutation. We conclude that routine application of RET proto-oncogene testing
should be included in all cases of apparent sporadic MTC.
http://mjiri.iums.ac.ir/article-1-624-en.pdf
2004-09-15
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IRAJ
NABIPOUR
1
From the Endocrine Research Center; Shaheed Beheshti University of Medical Sciences, Tehran, the Persian Gulf Health Research Center; Bushehr University of Medical Sciences, Bushehr and the Endocrine Research Center, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
FATEMEH
HAJI-GHASEMI
2
AUTHOR
SHAHRIAR
KIAI
3
AUTHOR
REZA
BARADAR-JALILI
4
AUTHOR
FEREIDOUN
AZIZI
5
AUTHOR