Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
PRODUCTION OF OXYTETRACYCLINE BY ISOLATED WILD TYPE IRANIAN STREPTOMYCES RIMOSUS
187
189
EN
MORTEZA
SATTARI
From The Department of Microbiology, Health Science Faculty, Tarbiat Modarres University, Tehran
NASRIN
MOAZAMI
the Biotechnology Center, Iranian Research Organization for Science and Technology, P.O.Box 15815-3338, Tehran
MOHAMMAD ESMAlL
ZULFAGHARI
the Pharmaceutical Research Center, Daru-Pakhsh Co., P.O. Box 13185-877, Tehran, Islamic Republic of Iran.
Production of oxytetracycline by an isolated strain of Streptomyces rimosus
from Iranian soil was investigated using a special fermentation medium. A
comparative study was performed with standard strain PTCC 1144 using the
following parameters optimum growth conditions with respect to time, pH, and
different amounts of corn steep liquor. Surprisingly, the production yield of the
wild strain was 20% more than the standard strain.
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
HEPATOPROTECTIVE ACTIVITY OF SILYMARIN IN RATS TREATED WITH HIGH DOSES OF ACE T YLSA LICYLIC ACID OR NAPROXEN
183
185
EN
EUGENE
J. KUCHARZ
From the Fourth Department of Internal Medicine, Silesian University, Scool of Medicine, ul. Edukacji 102, PL 43-100 Tychy, Poland.
THERESA
KOTT
Rats were treated with acetylsalicylic acid (150 mgikg b.w.per os daily)
or naproxen (125 mg/kg b.w. per os daily) for six weeks. Half of the rats
received silymarin (17.5 mgikg b.w. per os daily) in the last three weeks of
the experiment. It was found tliat administration of acetylsalicylic acid led to
signs of hepatic damage (an increase in serum bilirubin level, alanine aminotransferase
and ү-glutamyl transpeptidase activity) while the concomitant
administration of silymarin diminished the extent of the hepatic damage.
Naproxen was shown to be less toxic than acetylsalicylic acid, and its toxicity
was also reduced by silymarin. The obtained results suggest that
silymarin be administered to patients undergoing long-term treatment with
non-steroidal anti-inflammatory drugs in order to prevent hepatic damage,
but further studies are needed to elaborate on the clinical aspects of
silymarin treatment in those patients.
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
F EEDBACK REGU LATION OF COLONYSTIMULATING FACTOR PRODUCTION
177
182
EN
BAHRAM
GOLIAEI
From the Institute of Biochemistry and Biophysics, University of Tehran, Tehran, I.R. Iran.
MARYAM
TAHERI
AZRA
RABBANI
The production of colony-stimulating factors (CSF) is delicately controlled
through a complex network of humoral and environmental factors. We have
studied some of the mechanisms which regulate the production of CSF as
compared to general protein synthesis in the lung tissue ill vitro. When lung tissue
from mice was cultured for various times in serum free medium, the first detectable
level of CSF activity in the lung conditioned medium (LCM) appeared 6 hr after
initiation of the culture, continued to rise until 24 hr, and then levelled off for
several days. Under similar conditions protein synthesis did not level off, but
continuously rose after 24 hr. When the lung tissue which had been cultured
previously for 6,24, or 4X hr was recultured in fresh tissue culture medium, de IIOVO
synthesis of CSF occurred as judged by CSF synthesis inhibition and stimulation
studies. The amount of new CSF synthesized by these tissues decreased as the
initial culturing period increased from 6 to 4X hr. There was also a decrease in the
amount of total protein synthesis and release in the secondary lung cultures as a
function of the initial culturing period. Endotoxin stimulation of 24 or 4X hrcultured
lung tissues (plateau phase tissue) resulted in de IIOVO synthesis of CSF
by these tissues. However, when fresh lung tissue was cultured in 24 or 4X hr LCM,
no new CSF was produced by the fresh tissues, while under similar conditions,
protein synthesis by these tissues was significant as judged by double-labelling
experiments. On the other hand, n hr LCM was able to support both CSF
production and protein synthesis by fresh lung tissues. The results suggest at least
two distinct regulatory systems controlling CSF production by the lung ill vitro:
1- Aging which is responsible for general and nonspecific decrease in the rate of
protein synthesis and CSF production in this system, and 2-Feedback regulation
of CSF production by the level of CSF which is formed in the LCM.
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
DETERMINATION OF DOMINANT SEROVARS OF LISTERIA MONOCYTOGENES
173
175
EN
SAEED
MIRDAMADI
From the Biochemistry Department, Iranian Research Organization for Science & Technology (IROST) , P.O. Box: 15815-3538 Tehran, 15819
NASRIN
MOAZAMI
From the Biochemistry Department, Iranian Research Organization for Science & Technology (IROST) , P.O. Box: 15815-3538 Tehran, 15819
SHAHNAZ
RAFIEE TEHRANI
the Immunology Department, Tehran University of Medical Sciences, Tehran, I.R. Iran.
Serovars of Lisreria l1Iollocyrogelles were determined. Sera of aborted samples
(200) were collected from different hospitals in Tehran and were tested serologically
by immunofluorescent antibody methods (IF tests). 137 positive sera were
identified. Positive sera were tested against 12 serovars of Listeria l1Iollocytogelles
separately. Titers of antibody in patients' sera for all serovars were determined.
The results showed that the dominant serovars of L.l1Iollocyrogelles which caused
listeriosis in the samples were 4b, la, 2 and 3. None of the sera had antibodies
against serovars 4a, oa or (ib. Some of the sera which had high titers of antibody
against dominant serovars (4b, I a, 2 and 3), showed a faint result with serovars 4d,
4e,5 and 7.
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
EPIDEMIOLOGY OF PRIMARY DYSMENORRHEA IN KERMAN, IRAN
167
171
EN
MOHSEN
JANGHORBANI
From the Medical School, Kerman University of Medical Sciences, Kerman, IR. Iran.
SIMIN
ZARKOOB
The prevalence and factors influencing the prevalence and severity of
dysmenorrhea were studied in 768 female university students aged from
17.S to 44.2 years enrolled at the Kerman University of Medical Sciences
and two teachers' training colleges.
The overall prevalence of dysmenorrhea in this population was 72%
10.2% reported severe dysmenorrhea. The overall prevalence as well as the
prevalence of severe dysmenorrhea decreased with age. A statistically significant
association (P<0.05) was found between an early age of menarche
and an increase in the severity of dysmenorrhea. There was also a statistically
significant association (P<0.01) between the prevalence and severity of
dysmenorrhea and marriage, and married women had significantly less
dysmenorrhea (either in severity or prevalence) compared to non-married
subjects. The prevalence and severity of dysmenorrhea was not affected by
factors such as height, weight, body mass index, region of residence, gynecological
age, regularity of menstrual cycles, or duration or amount of menstrual
bleeding.
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
LEVELS OF SOLUBLE IL-2 RECEP TORS IN SERA OF IRANIAN PATIENTS WITH BEHCET'S DISEASE
163
166
EN
M
RAIANI
From the Department of Immunology Shariati Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
A
MASSOUD
From the Department of Immunology Shariati Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
F
DAVATCHI
From the Department of Rheumatology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
It has been shown that activated T-cells produce and release both IL-2 and IL-
2 receptors (lL-2R). The rate of IL-2 release is proportional to its cell surface
expression and state of cell activation. To clarify the molecular basis of this
immunological aberration, we analysed the amount of soluble IL-2R(SIL2-R) by
an ELISA technique in 68 patients with Behcet's disease (BD), 28 patient controls
(PC) and 31 normal controls (NC).
The data suggest that the amount of SIL-2R in BD is significantly higher
(P<O.005) than that of NC. The same differences were seen between PC and
NC(P<O.005). However the level of S IL-2R in BD and PC were similar.
This study suggests that inhibition of an immunoregulatory cytokine by its
soluble receptors might occur ill vivo, as the reduction ofS IL-2R levels approaching
control values preceded clinical remission.
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
ASSOCIATION OF HLA-B27 WITH ANKYLOSING SPONDYLITIS IN ISFAHAN, IRAN
159
161
EN
KM
ADIB
From the Immunogenetic Laboratory of Aliasghar Hospital, Isfahan University of Medical Sciences,
H
HOSSEINI
Using a standard microcytotoxicity (NIH) technique of tissue typing, the
HLA-B27 antigen was identified in 30 out of 34 patients (HH.2%) with classical
ankylosing spondylitis (AS), compared to 6 out of 70 controls (H.6%)
(P < 0.005).
We also found this antigen in 8 out of 76 (10.5%) patients with non-AS
arthritis.
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
CLINICA L FINDINGS IN PATIENTS WITH ACUTE AMEBIC PROCTOCOLITIS A ND EFFICA CY OF COMBINED THERAPY WITH SACCHAROMYCES BOULARDII
155
157
EN
K
YAZDANPARAST
From tile Dept. of Microbiology and the Dept. of Medicine, Shiraz University of Medical Sciences,Shiraz, Islamic Republic of lran
N
DEHBASHI
F
MANSOUR GHANAIE
Intestinal amebiasis has a worldwide distribution and is common in tropical
and subtropical areas. [n this prospective 7-month study, we studied the main
clinical findings in patients with acute amebic proctocolitis and the efficacy of
Saccharomyces boulardii (SB) treatment of these patients.Initially, 57 cases with
acute amebic proctocolitis were selected. The maximum occurrence of infection
was found to be in the 30-39 year old age group. The patients were then randomized
to two therapeutic regimens. The first included metronidazole 750 mg P.O. tid X 1 0
days and iodoquinol630 mg P.O. tid Xl 0 days, and the second was the latter plus
S. boulardii 250 mg P.O. tid x 10 days. The diarrhea, abdominal pain and fever
were significantly decreased after initation of therapy in patients receiving
regimen IT as compared with patients of regimen 1. Four weeks after the end of
treatment, the two groups were examined for carrier states (presence of amebic
cysts in stool, without symptoms). [n regimen I, the percentage of carriers was
[1).4%, but in the patients who received regimen IT, no carriers were found (P=
0.025).
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
AUTOANTIBODIES AGAINST THYR OID CONSTITUENTS AND CIC IN GRAVES' DISEASE ASSOCIATED WITH EXOPH THALMUS
149
153
EN
S
RAFIEI
From the Dept. of Immunology School of Medicine, Tehran University of Medical Sciences, Tehran
M
NAKHJEVANI
From the Dept. of Endocrinology, School of Medicine, Tehran University of Medical Sciences, Tehran
M
GHARAGOUZLOU
Bu Ali Sina University of MedicalSciences, Hamedan University, Hamedan, I.R.Iran.
32 patients (19 female. 13 male)• suffering from Graves' disease with
exophthalmus and five without exophthalmus were studied for various autoantibodies
including anti-thyroglobulin (ATAB). anti-microsomal antibody (AMAB).
anti-parietal antibodies (APAB) and also circulating immune complex (CrC). 43
normal subjects were tested concurrently.
Immunofluorescent technique was employed for autoantibody detection.
Frozen sections of human thyroid tissue and rat stomach were utilized as antigen
sources for thyroid and parietal CIC estimated by PEG (polyethylene glycol)
precipitation method and the specific classes of immunoglobulins were detected
by SRlD (single radial immunodiffusion).
The results were analyzed statistically. From the point of view of presence of
antibodies. there were significant differences between patients and control groups
(P= 0.(05). Comparing Graves' with or without exophthalmus. no significant
difference was seen between autoantibody levels. but higher titers of CIC were
elucidated in the latter group.
We will discuss that autoantibodies have an important role in thyroid
autoimmune diseases and also that CIC may have a critical role in the pathogenesis
of exophthalmus associated with Graves' disease
Iran University of Medical Sciences
Medical Journal of The Islamic Republic of Iran (MJIRI)
1016-1430
8
3
1994
11
1
CAROTID BODY TUMORS OUR EXPERIENCE WITH 20 PATIENTS
141
147
EN
MOHAMMADREZA
KALANTAR MOTAMEDI
From the Department of General and Vascular Surgery, Shohada Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran.
MOHAMMADHASAN
KALANTAR MOTAMEDI
In this report of 20 patients with 24 carotid body tumors which is the
largest series reported so far from Iran, we have evaluated the various characteristics
of this relatively rare tumor in our population and compared our
results with that of the literature.
Although the prevalence of the tumor is cited to be equal among both
sexes, we found a female to male ratio of 2.3 to I, as 14 of our patients were
female and 6 were male. The tumor was as equally frequent on the right as it
was on the left, and was bilateral in four cases. The average tumor size was
5.3 cm in diameter and was found to be malignant in four cases.
The results we obtained following the careful surgical technique which
is described in detail compares quite favorably with that in the literature: 3
cases of cranial nerve injury (12.5%) compared to an average of 45% nerve
injury in the reports, and no perioperative death versus 2% perioperative
mortality in the literature. Also we had no instances of stroke, transient
ischemia, Horner's syndrome, or other complications. We propose the surgical
technique detailed by the author as the method of choice for treatment of
carotid body tumors in Iranian patients.