2024-03-28T13:24:45+04:30 http://mjiri.iums.ac.ir/browse.php?mag_id=42&slc_lang=en&sid=1
42-751 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 A STUDY ON THE APPLICATION AND EFFICACY OF SOLVENT-DETERGENT (SID) TREATMENT IN THE PROCESS OF PURIFYING FACTOR VII FROM PROTHROMBIN COMPLEX H REZVAN S NASIRI K MOUSAVI M GOLABI The risks of transmitting viral infection by blood and plasma-derived products have long been known and still remain an area of concern. In this study, in the process of purifying human factor VII from prothrombin complex, SID treatment using tri-n-butyl phosphate and Tween 80 was employed and its capability and efficacy was studied. The results indicated that the process did not affect the biological function of the essential coagulation factors studied. In addition, the process was shown to be effective on enveloped viruses, where its inactivation factor for the model used, Herpes Simplex type I, was 5.5 logs. However, the procedure was not effective on poliovirus which was used as a model for non-enveloped viruses. It is therefore concluded that for increasing viral safety it is best to apply at least one more inactivating procedure which will be effective on non-enveloped viruses as well. Human Factor VII concentrate viral inactivation solvent-detergent treatment. 2002 11 01 179 182 http://mjiri.iums.ac.ir/article-1-751-en.pdf
42-750 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY OF PANCREATIC ISLET BETA CELLS OF DIABETIC RATS TREATED WITH ORAL VANADYL SULPHATE S AHMADI SM KARIMIAN M SOTOUDEH M BAHADORI Vanadium salts have been suggested as a possible therapeutic agent for the treatment of diabetes. The aim of the present study was to clarify histological and immunohistochemical changes that occur in the pancreatic beta (β) cells of vanadyl sulphate (VS)-treated streptozotocin (STZ) induced diabetic rats. Male Wi star rats were made diabetic by injecting a single intravenous dose of STZ (40 mg/kg) and were divided into two groups seven days after STZ injection. In the first group VS was administered via drinking water at a concentration of 1 mg/mL and treatment was maintained until normoglycemia appeared (DT). A second group of diabetic animals received distilled water for the same period and were considered as control diabetic (DC). One group of animals (NC) was injected intravenously with the same amount of vehicle as the diabetic rats and was considered as non-diabetic control. VS treatment was accompanied by amelioration of the signs of diabetes in DT rats while DC animals remained diabetic during this period. Hemotoxylin - Eosin stained pancreatic sections of DC rats showed a decrease in the number and size of islets and a disruption in their architecture. In DT rats the histological appearance of the islets was normal, their shape and size being within normal limits. In horseradish peroxidase procedure (using guinea pig antiserum to insulin as primary antibody) performed on pancreatic islet paraffin sections of rats, insulin immunoreactivity was found in the majority of the islets in DT rats while in the islets of DC rats immunoreactivity was rare. The results of this study indicated that amelioration of diabetes in vanadyl sulphate treated diabetic rats was accompanied with well preservation of islet structure and insulin immunoreactivity. Vanadyl Sulphate Pancreas Beta cell Diabetes. 2002 11 01 173 178 http://mjiri.iums.ac.ir/article-1-750-en.pdf
42-748 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 A SURVEY OF CELLULAR IMMUNITY, TOTAL T-LYMPHOCYTE, T-ACTIVE , B-LYMPHOCYTE A ND T-CELL FUNCTION IN RELATION TO PHYTOHEMAGGLUTININ (PHA) IN THALASSEMIC PATIENTS T ZANDIEH TZ7892000@Yahoo.com F TARABADI A TABATABAIYAN M IZADYAR The aim of this study was to evaluate the immune system and lymphocyte function in 41 Iranian β-thalassemic patients and 50 controls, ages ranging from 2 to 18 years. The patients consisted of 20 splenectomized and 21 non-splenectomized patients. They were treated with Desferal, and had received repeated blood transfusion. Laboratory investigations included measurement of total T lymphocytes, active T lymphocytes, B-Iymphocytes and function of lymphocytes treated with PHA. In this study we observed a significant reduction of active T lymphocytes and total T lymphocytes in the patient group compared to controls (p<0.005 & p<0.001), but there was no significant difference between splenectomized and non-splenectomized patients. Also in both groups, lymphocyte function was reduced against PHA (phytohemagglutinin) compared with the controls, and the numbers of B cells were increased. These results lead to the conclusion that the deficient immune system in β-thalassemia causes infectious diseases, which finally leads to death. Therefore, stimulation of the immune system as well as clinical treatment may prevent infectious disease in patients with β- thalassemia. Thalassemic patient Immune system Cytomegalovirus Blood transfusion. 2002 11 01 169 172 http://mjiri.iums.ac.ir/article-1-748-en.pdf
42-747 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 SPECIES IDENTIFICATION AND INV ESTIGATION OF VANCOMYCIN AND HIGH-LEVEL AMINOGLYCOSIDE AMONG CLINICAL ISOLATES OF ENTEROCOCCAL STRAINS SR MOADDAB A RAFI A study was performed to determine the species and antimicrobial susceptibility of 100 enterococcus strains from various clinical specimens as etiological agents at the Clinical Microbiology Laboratory, Istanbul University, Medical Faculty Hospital. Out of 100 enterococcal isolates, 86 were identified as E. faecalis, 11 as E. faecium, 1 as E. avium and 2 as E. raffinosus. By the disk diffusion tests 36 of 100 strains were found to be resistant to erythromycin, 32 to penicillin, 30 to ampicillin, 7 to ciprofloxacin, 6 to norfloxacin and 5 to nitrofurantoin. No resistance was observed to vancomycin or teicoplanin. Twenty-six strains showed high-level resistance to streptomycin (2000 )..lg/mL) and 13 strains exhibited high-level resistance to gentamicin (500 )..lg/mL). Ten of these strains had high-level resistance to both aminoglycosides. None of the strains produced beta -lactamase. 2002 11 01 165 168 http://mjiri.iums.ac.ir/article-1-747-en.pdf
42-746 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 PLASMID MEDIATED METAL AND ANTIBIOTIC RESISTANCE IN PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM BURN PATIENTS MOHAMMAD REZA SHAKIBAIE, shakibaie@arg3.uk.ac.ir Pseudomonas aeruginosa is a leading cause of bum infections, and antibiotic- resistant strains of this bacterium are emerging due to extensive application of antibiotics in the bum unit of hospitals. In this study 50 strains of P. aeruginosa were isolated from bum patients infected with this micro-organism in the bum unit of a general hospital in Kerman, Iran over one year [May 1999-ApriI2000]. Sensitivity/ resistance of the isolates for antibiotics and metals was determined by MIC test. 46% of the isolates were resistant to ciprofioxacin, kanamycin (K), gentamicin (Gm), tetracycline (Te) and chloramphenicol ( c ). 35% were resistant to amikacin (AN), ceftriaxone (Ceft), and cefotaxime (CTX), and 10% were resistant to imipenem (Imp) and piperacillin (PIP). The isolates exhibited varied MIC's to metal ions. 87% were sensitive to cadmium (Cd), 62% to lead (Pb), 91 % to mercury (Hg), 54% to zinc (Zn), 85% to chromium (Cr), and 83% to arsenate (Ars). Among them, strain 16 was found to be resistant to Pb, Cr, and Zn as well as Te, C, Gm, and K. Conjugation and transformation experiments revealed the transfer of Ter, Cr, Krand Gmr along with Pbr to a sensitive strain of P. aeruginosa PTCC1074.1 (Rif) but not to E. coli K12 HB101.1(Rifr). Subsequent plasmid isolation and agarose gel electrophoresis (0.7%) confirmed the presence of three-plasmid bands in strain 16 and the transconjugant. Furthermore, strain 16 accumulated a maximum amount of Pb (50µM) within 60 min. and reached a plateau afterwards. Pseudomonas antibiotic resistance lead resistance plasmid 2002 11 01 159 163 http://mjiri.iums.ac.ir/article-1-746-en.pdf
42-745 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 ANTI CARDIOLIPIN ANTIBODIES IN PATIENTS WITH ISCHEMIC EVENTS A GHAEMIAN M MOBINI A AJAMI To determine the presence of anticardiolipin (aCL) antibodies in patients with ischemic events, we designed a case-control study. We studied 33 patients with unstable angina, 33 male patients with myocardial infarction and 34 control subjects with no evidence of ischemic heart disease. Plasma samples were assessed for IgG anticardiolipin antibodies by enzyme-linked immunosorbent assay (ELISA). The levels of aCL were (mean±SD of optical density multiplied by 1000): 624±319, 486±318, and 239±202 for patients with unstable angina, myocardial infarction and controls, respectively (F= 15.74 and p=0.0000). High aCL levels were found more often in patients with acute ischemic events. Anticardiolipin antibodies Myocardial infarction Ischemic events. 2002 11 01 155 157 http://mjiri.iums.ac.ir/article-1-745-en.pdf
42-743 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 THE PROPHYLACTIC EFFECT OF LACTULOSE ON NEONATAL HYPERBILIRUBINEMIA BY ACCELERATION OF MECONIUM PASSAGE K SAKHA H SULTANI One of the causes of neonatal hyperbilirubinemia is increased reabsorption of bilirubin from meconium in the gastrointestinal tract. This occurs when the conjugated bilirubin which is excreted into the colon is 'unconjugated by beta glucuronidase activity, present in the neonatal intestine, which hydrolyzes bilirubin diglucuronide into unconjugated bilirubin, which subsequently is reabsorbed into the portal circulation, contributing to the bilirubin overload on hepatic excretory pathways. Thus, delayed passage of meconium can cause an elevation in the serum bilirubin level. We accelerated meconium transit by lactulose and evaluated the relationship between meconium passage, neonatal jaundice and bilirubin level. 150 newborns were selected after birth in Tabriz Al-zahra Hospital with special criteria. Half of them were given 4.5-5 mL lactulose by gavage 2 hours after birth. Time of meconium passage, appearance of jaundice and level of bilirubin were studied in both groups. Results showed that 40% of neonates in the study. group and 26.6% in the control group were non-icteric. Bilirubin level more than 12 mg/dL was seen in 28% of the study group and 53.4% of neonates in the control group. There was a statistically significant correlation between lactulose receivers and the control group (p=0.0028). This investigation showed that acceleration of meconium passage in newborns decreases the incidence of jaundice and hyperbilirubinemia. Jaundice Hyperbilirubinemia Lactulose Meconium. 2002 11 01 151 154 http://mjiri.iums.ac.ir/article-1-743-en.pdf
42-1638 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 KIDNEY TRANSPLANTATION IN CHILDREN: RESULTS OF TEN YEARS EXP ERIENCE IN IMAM REZA HOSPITAL R MAHDAVI M NAGHIB Advances in nephrology and pediatric urology have increased the number of children who survive renal disease and become candidates for renal transplantation. Ten years of experience in pediatric renal transplantation are reviewed to determine the rates of patient morbidity and graft survival. Of the 450 renal transplantations performed in Imam Reza Hospital (1989- 1999), fifty-one were done on children (6-18yrs.). Causes of renal failure included: reflux nephropathy, 8 cases neurogenic bladder, 5 cases posterior urethral valve, one case prune belly syndrome, 1 case small kidney due to chronic glomerulonephritis, 8 cases the remaining failures were of unknown etiology. All kidneys were harvested from living donors,3o related and unrelated.20 Immunosuppressive therapy was given with three drugs in all children: prednisolone, azathioprine, and cyclosporine, with the exception of 6 recipients of HLA identical siblings who did not receive cyclosporine. The Kaplan-Meier curve was constructed to assess graft and patient survival and the Log rank test was used to assess the effect of kidney source and date of renal transplant. Immediate dieresis occurred in all graft. Surgical complications included two urinary fistulae and one clinical lymphocele which were all repaired surgically. There were eleven acute rejections. The most common causes of graft failure were chronic rejection and recurrence of primary renal diseases. The graft survival rates after 1, 2, 5 and 10 years were 95%, 84%, 76%, and 62% respectively. By all measures, renal transplantation is still the treatment of choice for children with ESRD. Renal transplantation in children results in improvement in physical growth, mental development and rate of survival. Hypertension, chronic rejection, infection, obesity and medical noncompliance continue to be problematic. Graft Survival renal transplantation living donor children 2002 11 01 145 149 http://mjiri.iums.ac.ir/article-1-1638-en.pdf
42-1637 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 THE EFFICACY AND SA FETY OF INTERFERON ALFA FOR THE TREATMENT OF CHRONIC HEPATITIS B INFECTED SUBJECTS IN IRAN H FOROOTAN PISHBUARY emamddrc@mirmomen.com S MIRMOMEN S ALAMDARY H GHOFRANI MJ FARAHVASH M Y OSSEFI MASHHOOR F GHANAIE This preliminary study was designed to evaluate the effects of Heberon Alfa for the treatment of chronic hepatitis B infected subjects in Iran. A single center, open label, single treatment prospective study of Interferon Alfa (Heberon Alfa), 5 million units every other day for a period of 4 months, was performed between 1996 to 1998. A total number of 30 patients with histologically documented chronic hepatitis and positive serum HBsAg were included in the study. Serum ALT of all patients was greater than 1.5 times normal before start of therapy. Effect of therapy on aminotransferase activity and HBsAg, HBeAg seroconversion was monitored and all the patients underwent a second liver biopsy at the end of the study period. Mean age of patients was 35.5 ± 12 (17 to 60 years old) and 73% of patients were male. Most patients experienced adverse effects, but none warranted stopping the treatment. No serious or unexpected adverse event was reported during the study period. Thrombocytopenia was recorded in 2 patients. Liver biopsy showed a decrease in hepatic inflammation in 53.5% of patients, no change in 36.70/0 and increase in hepatic inflammation in 100/0 of patients after the treatment. Serum ALT returned to normal in 18 patients (60%), decreased in 7 patients (23.3%) and didn't change in 5 patients (16.7%). There was a strong correlation between serum ALT normalization and histological improvement. HBsAg became negative in 5 patients (16.6%). 10 patients had positive HBeAg prior to therapy, which became negative in 4 of then1 (40%) by the end of the study. The current study confirms the result of other clinical trials and indicates that Heberon Alfa is a safe and effective drug for the treatment of chronic hepatitis B infected subjects with histologically documented chronic hepatitis. Interferon chronic hepatitis B Heberon 2002 11 01 139 134 http://mjiri.iums.ac.ir/article-1-1637-en.pdf
42-1630 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 THE EFFECT OF URINE AND BLOOD METABOLIC RISK FACTORS ON CALCIUM STONE FORMATION A BASIRI A TABIBI M PARVIN In spite of vast improvements in urinary stone treatment (ESWL, PNL, ureteroscopy, etc.), metabolic workup concerning the existence of stone forming risk factors are of great importance and can lead to control and even prevention of urinary stone formation in these patients. In this analytical case-control study performed on 266 persons [110 normal persons (56 males and 54 females), 76 patients with one episode of stone formation (40 males and 36 females), and 80 patients with recurrent stone formation (40 males and 40 females)] aged between 30 to 45 (with an average of 37.6) in Shahid Doctor Labbafinejad Medical Center from May to July 1999, serum parameters and 24-hour urine parameters have been investigated and compared among the three groups. Results of this study revealed considerable differences in urinary calcium levels of these three groups, with and without considering sex (p<0.05). Averages of 24-hour urinary calcium calculated for normal, one episode and recurrent stone formers in male groups were 159±43, 219± 71, and 283± 74 mg/24h respective1y, and for normal, one episode and recurrent stone formers in female groups were 124±37, 190±58, and 287:t152 mg/24h respectively. Although 24-hour urine citrate in females obviously showed higher values than males, there was no significant difference among the studied groups of the same sex. Levels of serum calcium, potassium and magnesium between groups of females and 24- hour urine magnesium and phosphate levels between groups of males had statistical differences also (p<0.05 for all of the cases mentioned above). According to the results obtained from this study, it was realized that in the studied society levels of 24-hour urinary calcium which are higher than 200 mg/ 24h (sensitivity 80%, specificity 94% and FPR 6.4%) and calcium creatinine ratios of 24-hour urine which are higher than 0.17 (sensitivity 7.5%, specificity 88.1 % and FPR 11.9%) can be regarded as hypercalciuria. However, the results of this study should be confirmed by more general and extended studies. Kidney stone Hypercalciuria Hypocitraturia Hypomagnesuria 2002 11 01 133 137 http://mjiri.iums.ac.ir/article-1-1630-en.pdf
42-742 2024-03-28 10.1002
Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 DOPPLER-DERIVED RIGHT VEN T RICU L AR MYOC ARD IAL PERFORMANCE IN DEX IN NEON ATES: N ORMAL VALUES E MALAKAN RAD malakan_rad @kaums.a c.ir N MOMTAZMANESH Momtazmanesh@kaums.ac.ir Doppler-derived myocardial performance index (MPI) , defined as the s um of isovolumetric contraction and relaxation durations divided by ejection time, is an easily measured and reproducible index that shows both systolic and diastolic myocardial function. The goal of this study was to define normal values of right ventricular MPI in neonates in the first 48 to 72 hours of life. Fifty-one quiet or sleeping healthy and term neonates underwent complete M-mode, two-dimensional color-Doppler echocardiographic examination and RV MPI was calculated in them. Statistical analysis was performed using SPSS software. A P value less than 0.05 was considered as significant. RV MPI was shown to be 0.23±0.14 in healthy neonates. There was no correlation between RV MPI and either age or weight of the newborn infants. While this value closely resembles the results of some researchers, it is lower than that previously reported by other investigators in healthy children. The lower value of RV MPI in neonates may be possibly related to the higher pulmonary arterial pressure or right ventricular mass in the first few days of life in neonates. Further study to evaluate the effect of pulmonary arterial pressure and right ventricular mass on RV MPI is recommended. RVMPI neonates normal values 2002 11 01 129 132 http://mjiri.iums.ac.ir/article-1-742-en.pdf
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Medical Journal of The Islamic Republic of Iran (MJIRI) Med J Islam Repub Iran 1016-1430 2251-6840 10.18869/mjiri 2002 16 3 A 20-YEAR SURVEY OF INFECTIOUS COMPLICATIONS IN 64 PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY A AGHAMOHAMMADI A FARHOUDI M MOEIN Z POURPAK N REZAEI rezaei_nima@yahoo.com K ABOLMAALI M MOVAHEDI M GHARAGOZLOU B MIRSAEIDGHAZI M MAHMOUDI Common variable immunodeficiency (CV ID) is a heterogeneous primary immunodeficiency disorder, characterized by hypogammaglobulinemia and increased susceptibility to recurrent bacterial infections. To determine the spectrum of infectious complications in patients with common variable immunodeficiency (CV ID), we reviewed the hospital records of 64 CVID patients, who were diagnosed in Children's Medical Center during a period of 20 years. Among our patients, there were 38 males and 26 females, with a median age of 12 years (2-42 years) at the time of study. The median age of diagnosis was 6.1 years, with an average diagnostic delay of 5.2 years in our patient's group. Almost all of our patients have suffered from acute and recurrent infections, particularly in the respiratory and gastrointestinal systems. The majority of patients (82.5%) had pneumonia prior to diagnosis. The other infectious complications seen in our patients were as follows: recurrent otitis media (54%), recurrent sinusitis (50.8%), diarrhea (65.1 %), and bacterial meningitis (11.1 %). Unusual or opportunistic infections were also seen in some of our patient population, including oral candidiasis in 10 patients, and Pneumocystis carinii pneumonia in 2 subjects. Based on this study, we suggest that hypogammaglobulinemia should be considered in any patient with a history of recurrent infections in different organs, and such patients should have a full assessment of the immune system. Common variable immunodeficiency (CYID) Infection Complication hypogammaglobulinemia. 2002 11 01 123 128 http://mjiri.iums.ac.ir/article-1-741-en.pdf