TY - JOUR T1 - The Value of Sputum Polymerase Chain Reaction for Detection of Nontuberculous Mycobacteria in Cystic Fibrosis Patients withNegative Nontuberculous Mycobacteria Sputum Culture TT - JF - MJIRI JO - MJIRI VL - 36 IS - 1 UR - http://mjiri.iums.ac.ir/article-1-7205-en.html Y1 - 2022 SP - 61 EP - 64 KW - Cystic Fibrosis KW - Nontuberculous Mycobacteria KW - Polymerase Chain Reaction KW - Sputum N2 - Background: Cystic Fibrosis (CF) is a life-threatening autosomal recessive disease. The purpose of this study was to evaluate the value of Polymerase Chain Reaction (PCR) in CF patients with Nontuberculous Mycobacteria (NTM) negative sputum culture. Methods: This is a descriptive cross-sectional study. The population included all children with CF, aged between 5 - 18 years old, with an NTM negative sputum culture. The patient's sputum samples were sent for smear and culture of NTM, RFLP PCR, and PCR sequence. Results: In total, 57 CF patients with negative NTM sputum culture were enrolled. Nine patients (15.78%) had positive sputum PCR for NTM. Among these strains, Mycobacterium simiae was the most common one with 5 cases (8.77% of total positive cases). Conclusion: PCR can be used as an alternative diagnostic method for NTM in CF patients with negative NTM sputum culture, always under clinical suspicion of the disease. M3 10.47176/mjiri.36.7 ER -